African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Table of Content: July 2019; 18(27)

July 2019

Molecular characterization of some bacteria isolated from munitions contaminated sites in Kachia Military Firing Range, Kaduna State, Nigeria

Environmental pollution is principally caused by human activities that usually result in the release of man–made pollutants such as biological, chemical and radioactive in such states as solid, liquid and gaseous substances into the biosphere via, land, water and air. Globally, the increasing rate of environmental contamination by ammunition/explosives resulting from their increasing deployments in the rising...

Author(s): A. I. Alhaji, B. C. Onusiriuka, D. B. Maikaje, J. A. Appah, P. A. Vantsawa, Y. Magaji, A. A. Haroun, E. E. Oaikhena and E. O. Oladapo

July 2019

Genetic diversity in some Ghanaian and Malian sorghum [Sorghum bicolor (L) Moench] accessions using SSR markers

The study was carried out to assess genetic diversity among forty-one sorghum accessions obtained from Savanna Agricultural Research Institute (SARI), Nyankpala, Northern Region of Ghana and the germplasm collection of Department of Molecular Biology and Biotechnology, University of Cape Coast. Genetic diversity and relationship among the forty-one accessions were evaluated using 22 microsatellite primers. The 22...

Author(s): Andrews Danquah, Isaac K. A. Galyuon, Emmanuel P. Otwe and Daniel K. A. Asante.

July 2019

Effective method to control Vibrio mimicus infection in channel catfish Ictalurus punctatus

Recently, a skin ulcerative disease caused by Vibrio mimicus has led to heavy economic losses in catfish, including yellow catfish, southern catfish, and Zhengchuan catfish in China. Currently, there was no effective method of controlling the outbreak of this disease. In this study, the bacterial isolates were obtained from dying channel catfish and identified as V. mimicus, which consist of formalin-inactivated V....

Author(s): Yan-Wei Li, Xiang Zhang , Yi-Jie Cai, Shu-Yin Chen, Hong-Yan Sun# and Xue-Ming Dan#

July 2019

Morphological and molecular screening of rice accessions for salt tolerance

The ever-increasing demand for rice raises the need to increase productivity by using salt tolerant varieties on saline soils. In this study, 12 rice accessions were screened for tolerance to salt at seedling stage using morphological and molecular methods. The study was carried out in a hydroponic system using Hoagland solution. Scoring was done using the modified standard evaluation score (SES) system after 14 days of...

Author(s): Uyoh E. A., Ntui V. O., Umego C., Ita E. E., and Opara C.  

July 2019

Enhancement of phytoremediation efficiency of Acacia mangium using earthworms in metal-contaminated soil in Bonoua, Ivory Coast

In this paper, the impact of Pontoscolex corethrurus on Acacia mangium growth and its phytoremediation efficiency in metal-contaminated soils were studied in a greenhouse pot culture under four treatments: non contaminated soil-non inoculated, control (C); non contaminated soil-inoculated (NcI); contaminated soil-non inoculated (CNi); contaminated soil-inoculated (CI). The results showed that A. mangium growth...

Author(s): BONGOUA-DEVISME Affi Jeanne, AKOTTO Odi Faustin, GUETY Thierry, KOUAKOU Sainte Adélaïde Ahya Edith, NDOYE Fatou and DIOUF Diégane  

July 2019

Review of microfluidics approaches to mimic the kidney

The use of microfluidics and nanotechnology in bioscience is gaining favor among the next generation of bioscience and clinical researchers, and this field has expanded to include organ-specific applications and modeling. While certain organ-specific microfluidic chips/applications, such as those in the heart and liver, are ubiquitous in the peer-reviewed literature, the kidneys require more focus and attention to drive...

Author(s): Zach Odeh, and Hongli Lin,

July 2019

Role of mucous cell and p53 protein in gastroprotective activity of methanolic extracts of Ageratum conyzoides Linn in rats

Ageratum conyzoides Linn is a medicinal plant used for diverse ethnomedicinal applications including anti-ulcer treatment. Usually, protection of gastric mucosa from injury or ulceration is dependent on the efficacy of intrinsic or induced protective factors against erosive effects of aggressive factors. In this study, our aim was to ascertain the gastroprotective activity of methanolic leaf extracts of A. conyzoides L....

Author(s): Omotoso D. R., Ajeigbe K. O., Owonikoko W. M., Okwuonu U. C., Akinola A. O., Daramola O. O., Adagboyin O. and Bienonwu E. O.

July 2019

Modified protocol for RNA isolation from different parts of field-grown jute plant suitable for NGS data generation and quantitative real-time RT-PCR

High quality RNA extraction from field-grown jute plants can be difficult due to the presence of complex polyphenolic, polysaccharide and waxes. But isolation of RNA in high quality is a basic need in plant genetics, genomics, transcriptomics, molecular biology and related physiological investigations. Here, cetyl trimethyl ammonium bromide (CTAB) based modified RNA isolation protocol suitable for isolating high quality...

Author(s): Rasel Ahmed, Md. Sabbir Hossain, Md. Samiul Haque, Md. Monjurul Alam, Md. and Shahidul Islam,

July 2019

DNA barcoding of Ghanaian fish species: Status and prospects

Ghana’s waters are biologically diverse with different fish species due to different ecological habitats and niches harboring several cryptic fish species. However, the fish production has reduced over the past decade; hence an urgent conservation and management strategies are required to save Ghanaian fishery such as accurate identification of species to formulate species-specific conservation and management...

Author(s): Gyamfua Afriyie, Shunkai Huang, Zhongdian Dong, Yusong Guo, Felix K. A. Kuebutornye, Christian Ayisi Larbi, Berchie Asiedu and Zhongduo Wang

July 2019

Genomic sequencing and recombinant expression of proinsulin isolated from cow and buffalo in Pakistan

This study was conducted to determine the heterologous expression of eukaryotic gene in Escherichia coli variable. The expression of Pakistani buffalo (Bubalus bubalis) and cow (Bos taurus) proinsulin genes in BL21 codon plus cells is 30% of total cellular protein. Total RNA was isolated from pancreatic tissues of local (Pakistani) breeds of buffalo (B. bubalis) and cow (B. taurus), converted to cDNA and cloned in a T/A...

Author(s): Farheen Aslam, Hooria Younas and Saima Iftikhar Bajwa  

July 2019

Optimization studies of chitin and chitosan production from Penaeus notialis shell waste

Optimization studies of extraction of chitin and chitosan from pink shrimp (Penaeus notialis) shell waste and of the degree of deacetylation (DDA) of extracted chitosan were investigated via the Box-Behnken design of experiments using response surface methodology. Robust quadratic models for predicting the extraction yields of chitin and chitosan and DDA of chitosan were obtained. These models were verified by...

Author(s): Amoo K. O., Olafadehan O. A. and Ajayi T. O.  

July 2019

Somatic embryogenesis and regeneration of Kenyan wheat (Triticum aestivum L.) genotypes from mature embryo explants

Wheat (Triticum aestivum L.) improvement via genetic transformation depends on an efficient regeneration system for recovery of transgenic events. This study reports a somatic embryogenesis-based regeneration system for two Kenyan wheat genotypes (Eagle 10 and Njoro bread wheat II) from mature embryos. The study investigated the efficiency of mercuric chloride, commercial bleach, and chlorine gas in surface sterilizing...

Author(s): Mark Ochieng Adero, Easter David Syombua, Lydia Kwamboka Asande, Nelson Onzere Amugune, Eliud Sagwa Mulanda and Godwin Macharia