African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12412

Table of Content: May 2021; 20(5)

May 2021

Precise assessment of angular leaf spot severity using the Leaf Doctor app for common beans

Angular leaf spot (ALS) is one of the most severe diseases of common bean. However, there is as yet no standardization of the phenological stage, inoculum concentration, diagrammatic scale (DS), and day of assessment for screening ALS resistance in bean lines. The present study aimed to evaluate the potential for assessment of ALS severity in common beans using the Leaf Doctor app and inoculation at the V2 phenological...

Author(s): Caléo Panhoca de Almeida, Gabriel Francesco Janini Bonfante, Jean Fausto de Carvalho Paulino, Bianca Cristina de Deus, Flávia Rodrigues Alves Patrício, Sérgio Augusto Morais Carbonell, Alisson Fernando Chiorato and Luciana Lasry Benchimol-Reis

May 2021

Synthesis, physicochemical and antimicrobial properties of rhenium (I) tricarbonyl complexes of isatin Schiff bases

The Schiff base of isatin and sulphanilamide (L1; C14H11N3O3S) was prepared and reacted with Re(CO)5X (X = Cl and Br) in toluene to give [Re(C14H10N2O)(CO)3X] (X = Cl, 1 and X = Br, 2). The prepared Schiff base of isatin and 4-methoxyaniline (L2; C15H12N2O2) was reacted with Re(CO)5X in toluene to give [Re(C14H10N2O)(CO)3X], where X = Cl, 3 and X = Br, 4. L2 and Re(CO)5Br were refluxed in dry toluene under nitrogen and...

Author(s): Adebomi A. Ikotun, Abimbola A. Owoseni and Gabriel O. Egharevba  

May 2021

Production of truncated peptide (cellobiohydrolase Cel6A) by Trichoderma reesei expressed in Escherichia coli

Enzymatic cellulose hydrolysis is an important step for the production of second-generation biofuels. The filamentous fungus Trichoderma reesei is among the most important organisms for obtaining cellulolytic enzymes. The Cel6A (CBH II) cellulase from T. ressei plays an important role in cellulose hydrolysis and acts on the non-reducing end of cellulose, in contrast to Cel7B (CBH I), which acts on the reducing end of...

Author(s): Miriam Shirley Tellez Calzada, Juan Antonio Rojas Contreras, Jesus Bernardo Paez Lerma, Nicolas Oscar Soto Cruz and Javier López Miranda

May 2021

Quantification and characterization of faecal sludge produced in Kara

In Kara (Togo), as in most major sub-Saharan African cities, there is very little sewerage and autonomous systems are the most commonly used. These autonomous systems produce faecal sludge that must be drained and treated adequately in order to preserve the health of the population and protect the environment. Indeed, the risks are real, given the use of fresh faecal sludge in agriculture and the potential dangers of...

Author(s): Piyabalo KODOM, Edem Komi KOLEDZI, Nitale M’Balikine KROU, Kwamivi N. SEGBEAYA and Bouwèdèo TOI BISSANG

May 2021

A rapid detection method on-site for Zygosaccharomyces based on loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick (LFD)

The Zygosaccharomyces is notorious for its remarkable spoilage characteristics. In the present study, the visual and rapid identification of the genus Zygosaccharomyces was performed by a loop-mediated isothermal amplification (LAMP) assay using specific primers in Mini Dry Bath within 30 min at 65°C followed by a lateral flow dipstick (LFD) detection. The sensitivity evaluation revealed LAMP-LFD assay with...

Author(s): Yang Liu≠, Jun Wu≠, Li-Qing Chen, Ya Shi, Hong-Xia Zhao, Wei Chen, Hong Li and Lu-Ping He,

May 2021

Synthesis, characterization and antibacterial activity of AgNPS mediated by Ocimum sanctum

This research focused on isolation of Silver Nano Particles (AgNPs) using leaf filtrate of Ocimum sanctum and evaluation of their antibacterial action against Bacillus aureus, Staphylococcus aureus and Escherichia coli. It is known that plant extracts act as reducing agents, stabilizers and as capping agents. The O. sanctum plant was specifically selected due to its medicinal properties and abundant availability. The...

Author(s): A. V. Narasimha Swamy and R. Srinu Venkat Rao

May 2021

Optimization of growth media for maximal production of insecticidal vegetative protein (vip3A) from Bacillus thuringiensis and its activity against black cutworm (Agrotis ipsilon)

Bacillus thuringiensis is a Gram-positive bacterium naturally found in soil, water and grain dust. B. thuringiensis has been used for decades as biological control for pests of economical and medical importance. The aim of this study was to test different growth media for the optimal production of vegetative insecticidal protein (Vip3) by B. thuringiensis recovered from the local environment. The local B. thuringiensis...

Author(s): Fatimah Alshehrei, Doaa K. El-Ghareeb, Ashraf El Baz, Sameer Qari, Hanafy Hamza, Hussein H. Abulreesh, Hesham A. Malak, Rehab Elmougy and Gamal E. H. Osman,