African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Table of Content: October 2003; 2(10)

October 2003

Genetic transformation of forest trees

  In this review, the recent progress on genetic transformation of forest trees were discussed. Its described also, different applications of genetic engineering for improving forest trees or understanding the mechanisms governing genes expression in woody plants.   Key words: Genetic transformation, transgenic forest trees, gene expression.  

Author(s): Diaga Diouf

October 2003

Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs

  Two DNA-based fingerprinting techniques, simple sequence repeats (SSR) and random amplified polymorphic DNA (RAPD) analyses, were applied in sorghum germplasm analysis to compare suitability for quantifying genetic diversity. Twenty-two sorghum genotypes, representing an array of germplasm sources with important agronomic traits, were assayed for polymorphism using 32 RAPD primers and 28 sets of sorghum SSR...

Author(s): Agrama H. A., and Tuinstra M. R.

October 2003

Sequence heterogeneity of the envelope-like domain in the Egyptian cotton Gossypium barbadense

  The current study aimed to investigate the evolution of env-like sequences in the Egyptian cotton Gossypium barbadense. DNA sequence determination and analysis ofenv-like sequences revealed that these sequences are heterogeneous in G. barbadense. The observed sequence diversity, however, seems to preserve the coding information. Phylogenetic analysis demonstrated that plant env-like...

Author(s): Abdel Ghany A. Abdel Ghany and Essam A. Zaki

October 2003

Influence of nitrogen, phosphorus and potassium application on the yield of maize in the savanna zone of Nigeria

  Field trials testing the effects of five rates each of N, P and K application on three hybrid and two open-pollinated maize varieties were conducted in three separate experiments on an Arenic haplustalf (USDA) at Ilora in the derived savanna and Typic paleustalf (USDA) at Mokwa in the southern guinea savanna of Nigeria. The hybrid maize varieties planted were 8516-12, 8321-18 and 8329-15 and were compared...

Author(s): Kogbe J. O. S. and Adediran J. A.

October 2003

The Contribution of food plants to the growth, development and fecundity of Zonocerus variegatus (L)

  The performance of the variegated grasshopper, Zonocerus variegatus (L) fed on different food plants namely cassava (Manihot esculenta), pawpaw (Carica papaya)and acalypha (Acalypha wilkesiana) and a mixture of these plants (mixed diets) from 5th nymphal instar to adult was investigated. Survivor and longevity of nymphs and as well as the reproductive performance of...

Author(s): Idowu A.B. and Sonde O.A.

October 2003

Citric acid production from whey with sugars and additives by Aspergillus niger

  Citric acid (CA) production by Aspergillus niger ATCC9642 from whey with different concentrations of sucrose, glucose, fructose, galactose  riboflavin, tricalcium phosphate and methanol in surface culture process was studied. It was found that whey with 15% (w/v) sucrose with or without 1% methanol was the most favourable medium producing the highest amount (106.5 g/l) of citric acid....

Author(s): Murad A. El-Holi and Khalaf S. Al-Delaimy

October 2003

Removal of Cu (II) and Zn (II) ions from wastewater by cassava (Manihot esculenta Cranz) waste biomass

  The ability of cassava waste biomass (untreated and acid treated) to remove heavy metals (Cu(II) and Zn(II) ) from single-ion solution and wastewater was investigated. All experiments were conducted using 10mM solutions of mixed metal ions of CuSO4.5H2O and ZnCl2. The uptake capacities of the two metal ions tested on the untreated and acid treated cassava waste biomass were 71.3 and 85.2 mg/g for Cu(II), and...

Author(s): HORSFALL, M. Jnr, ABIA, A. A. and SPIFF, A.I.

October 2003

Mycoflora and mycotoxins in kolanuts during storage

  The mycoflora, levels of afllatoxins and the presence of ochratoxin A and zearalenone in nuts of Cola acuminata and C. nitida were determined immediately after curing and after 3, 6 and 9 months of storage in leaf-lined baskets. Five field fungi and 11 storage fungi were isolated. Aspergillus, Penicillium and Fusarium were the predominant genera. None of the target...

Author(s): L. O. ADEBAJO and O. J. POPOOLA

October 2003

Mycology and spoilage of retail cashew nuts

Thirty-two samples of retail cashew nuts from Lagos, Nigeria were examined on two media. The pH values (5.1-6.3) of all the samples were conducive for fungal growth and mycotoxin production. Moisture content levels ranged between 4.1 and 6.8%. Fifteen samples had moisture contents up to or above 5.8%, the highest level estimated to be ‘safe’ for the storage of the nuts. Fourteen fungal species, mostly...

Author(s): L. O. Adebajo and S. A. Diyaolu

October 2003

Antibiotic resistant Staphylococcus aureus in Abia State of Nigeria

  A total of 70 ear and nasal swab samples collected from 35 persons, 16-hospital population and 19 non-hospital population was examined for presence ofStaphylococcus aureus. Eighty percent of the population studied were found to be carriers of S. aureus. Of the 28 positive cases, 35.7% were carriers of S. aureus in both the ear and nostrils, while 14.3% and 50.0% had it only in their ear and...

Author(s): Chigbu Chinwe .O. and Ezeronye O.U.

October 2003

Antimicrobial, heavy metal resistance and plasmid profile of coliforms isolated from nosocomial infections in a hospital in Isfahan, Iran

  The antimicrobial, heavy metal resistance patterns and plasmid profiles of Coliforms (Enterobacteriacea) isolated from nosocomial infections and healthy human faeces were compared. Fifteen of the 25 isolates from nosocomial infections were identified asEscherichia coli, and remaining as Kelebsiella pneumoniae. Seventy two percent of the strains isolated from nosocomial infections possess multiple...

Author(s): Vajiheh Karbasizaed, Naser Badami, Giti Emtiazi

October 2003

Plasmodium falciparum malaria resistance to chloroquine in five communities in Southern Nigeria

  Chloroquine is still a first-line antimalarial drug in uncomplicated falciparum malaria. Increasing resistance to chloroquine has been reported in many parts of Nigeria. Clinical and parasitological responses and classes of resistance to chloroquine in falciparum malaria in five communities in Delta region, southern Nigeria were assessed. Chloroquine was administered to 218 patients with uncomplicated P....

Author(s): Patrick O Erah, Gertrude Arienmughare and Augustine O Okhamafe

October 2003

Prevalence of Bartonella infection among patients with fever

  Bartonella henselae has been associated with an increasing spectrum of clinical syndromes including cat scratch disease. The prevalence of Bartonella infection among patients with unexplained fever in San Francisco was much greater than has previously been documented. However, out of 29 Japanese children with fever of unknown origin, only one had serum IgG antibody to B....

Author(s): Kei Numazaki

October 2003

Glycyrrhizin therapy for viral infections

  Glycyrrhizin (GL) was reported as the most active in inhibiting replication of the severe acute respiratory syndrome (SARS)-associated coronavirus. Therapeutic effect of GL for liver dysfunction associated with cytomegalovirus (CMV) infection in immunocompetent individuals was evaluated. Liver dysfunction in 4 cases improved and CMV disappeared from urinary samples after administration of GL intravenously by...

Author(s): Kei Numazaki