African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Characterization of lactic acid bacteria isolated from poultry farms in Senegal

Coulibaly Ibourahema1*, Robin Dubois Dauphin1, Destain Jacqueline1 and Philippe Thonart1, 2
  The group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known and these organisms have been characterized extensively by using different techniques. In this study, thirty lactic acid bacterial strains were isolated from soils chicken faeces and feathers. A total of nineteen isolates were obtained and by sequential screening for catalase activity and Gram-staining, eight were determined to be LAB out of which six were established to be homofermentative by the gel plug test. Five isolates were identified by use of the API 50CHL kit and four Lactobacilli strains and one Lactococci strain were selected to study their growth and lactic acid production profiles in a time course experiment. The Lactobacilli strains, both isolated from faeces, produced higher amounts of cells and lactic acid from soils as compared to the lactococci strain isolated from feathers. L (+)-lactic acid is the only optical isomer for use in pharmaceutical and food industries because is only adapted to assimilate this form. The optical isomers of lactic acid were examined by L (+) and D (-) lactate dehydrogenase kit. Lactobacilli strains produced combination of both optical isomers of lactic acid. Among them, Lactobacillus casei subsp. casei produced the low amount of D (-)-lactic (2%). The optimum rates of glucose for lactic acid production by Lactobacillus strains were 180 and 120 g/l for Lactobacillusplantarum and Lactobacillus paraplantarum, respectively.   Key words: Characterization, lactic acid bacteria, Lactobacillus, L (+)-lactic acid, D (-)-lactic acid, optical isomers.
Email: [email protected]

  •  Accepted: 16 May 2008
  •  Published: 17 June 2008

How to cite this article

APA /
Adebiyi, A. O., Oladipo, I. C., Ayandele, A. A., Adebiyi, A. P., & Adelowo, O. O. (2008). Characterization of lactic acid bacteria isolated from poultry farms in Senegal. African Journal of Biotechnology , 7(12), 2006-2012.
Chicago /
Coulibaly Ibourahema, Robin Dubois Dauphin, Destain Jacqueline and Philippe Thonart, . "Characterization of lactic acid bacteria isolated from poultry farms in Senegal." African Journal of Biotechnology 7, no. 12 (2008): 2006-2012.
MLA /
Coulibaly Ibourahema, et al. "Characterization of lactic acid bacteria isolated from poultry farms in Senegal." African Journal of Biotechnology 7.12 (2008): 2006-2012.