Aim: The objective of this study was to investigate the staining ability of the extract of the stalk of Sorghum bicolor as a counterstain on Haematoxylin.
Methods: Tissue blocks of trachea, oesophagus and ileum were retrieved from the Autopsy Block
Archive of the Department of Pathology, University of Ilorin Teaching Hospital Ilorin. Eight (8) serial sections labeled A to H were made from each block and stained with Harris haematoxylin. Section A was counterstained with 1% ethanolic eosin, as control. Different preparations of extracts of the stalk of
Sorghum bicolor were used to counterstain sections of Groups B to H as follows: B; 5% aqueous extract with 0.5g Potash, C; 5% aqueous extract, D; 5% extract in 70% ethanol, E; 10% extract in 70% ethanol, F; 5% extract in absolute ethanol, G; 10% extract in absolute ethanol and H; 5% aqueous extract with 40μl of acetic acid.
Results: The stalk extracts of S. bicolor stained the cytoplasm in shades of pinkish brown
Conclusion: This study established the cytoplasmic counter-staining ability of stalk extracts of S. bicolor, and the 10% extract in absolute ethanol had the closest resemblance to the Group A, the control group. It is therefore suggested that 10% extract in absolute ethanol can be substituted for eosin due to its domestic availability, ease of preparation and use, and above all, its good cytoplasmic contrast with the nuclear stain.
Keywords: Sorghum bicolor, Counterstain, Extract, Eosin.
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