African Journal of
Environmental Science and Technology

  • Abbreviation: Afr. J. Environ. Sci. Technol.
  • Language: English
  • ISSN: 1996-0786
  • DOI: 10.5897/AJEST
  • Start Year: 2007
  • Published Articles: 1126

Full Length Research Paper

Effect of essential oils extracted from Satureja calamintha, Mentha pulegium and Juniperus phoenicea on in vitro methanogenesis and fermentation traits of vetch-oat hay

Arhab Rabah1,2*, Khenaka Karima1, Leulmi Nassima1, Belaidi Hakim2, Harzallah Besma1 and Bousseboua Hacene1
1Laboratoire de Génie Microbiologique et Applications, Université Mentouri, BP 325 route de Aïn-El-Bey 25 017 Constantine. Algérie. 2Département des Sciences de la Nature et de la Vie, Faculté des Sciences Exactes et des Sciences de la Nature et de la Vie, Université Larbi Ben Mhidi, Oum El Bouaghi,  Algérie.
Email: [email protected]

  •  Accepted: 24 April 2013
  •  Published: 30 April 2013


The impact of inclusion of essential oils (EO) extracted from Juniperus phoenicea, Satureja calamintha and Mentha pulegium at three levels: 0, 1.66, 3.33 and 6.66 μl/ml of incubation medium, on methane production and rumen fermentation traits of vetch-oat hay was studied in in vitro gas production test, using 200 mg of substrate in a 60 ml graduated syringes. Gas production was recorded at 3, 6, 9, 24, 48, 72 and 96 h.  Methane was measured at 3, 24 and 96 h, while pH and ammonia N were analyzed just at 24 h of incubation. At 24 h of fermentation, all doses of EO reduced significantly total gas and methane production (P < 0.05). At the highest concentration and after 24 h, methanogenesis was decreased by 60.98, 57.70 and 64.64% for J. phoenicea, M. pulegium and S. calamintha, respectively. This effect was more pronounced at the end of fermentation (96 h) where reduction in methane production was superior to 72%. However, addition of EO increased pH and decreased significantly ammonia concentration, mainly, for high EO concentrations. The results indicate that these EO had a potential to reduce methanogenesis in the rumen, but further in vitro and in vivo trials are required to search optimum dose which reduce methane production without adversely changing dietary fermentation and rumen function.


Key words: Methane, ammonia, essential oils, Satureja calamintha (Calament), Mentha pulegium (fliou, Menthe pouliot), Juniperus phoenicea (Arar, Genévrier)in vitro gas production test