Abstract

 

Primers SC1 and SC2 primarily developed to be specific to Scutellospora castanea were used to unambiguously discriminate this species among endogenous arbuscular mycorrhizal fungi collected in Côte d’Ivoire. The cloning of genomic DNA from this fungus allowed the isolation of repeated DNA sequences. Sixteen inserts were randomly selected from the genomic bank and hybridized with total DNA from the spores of the identified fungus as probe. Repeated DNA sequences were obtained in the form of intense signals. Five fragments gave intense signals of hybridization showing that they are repeated elements in the genome. One of these sequences ScaCI2 having 1000 pb was characterized. It is dispersed in the genome of this AM fungus and accounts for 0.03% of this one.

 

Key words: Arbuscular mycorrhizal fungi, Scutellospora castanea, Côte d’Ivoire, detection.