African Journal of
Environmental Science and Technology

  • Abbreviation: Afr. J. Environ. Sci. Technol.
  • Language: English
  • ISSN: 1996-0786
  • DOI: 10.5897/AJEST
  • Start Year: 2007
  • Published Articles: 1083

Full Length Research Paper

Effect of gas recirculation intensity and various temperatures on hydrogenotrophic methanogens activity in chemostat fermentation using H2/CO2 as substrate

Olga Yolande Ako1* and Yutaka Kitamura
1UFR des Sciences et Gestion de l'Environment, University of Nangui Abrogoua, Abidjan, Côte d’Ivoire. 2Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan.  
Email: [email protected]

  •  Received: 02 October 2014
  •  Accepted: 24 February 2015
  •  Published: 20 May 2015


The influence of mixing H2/CO2 gas recirculation on the performance of hydrogenotrophic methanogens activity in continuous culture was studied at 37 and 20°C. Chemostat fermentation was used at laboratory scale to determine the bioconversion rate of H2/CO2 mixture gas to methane under different mixing rates. On comparison with continuous mixing, intermittent mixing at 45 min/h provided a better methane production, 1.94 ± 0.06 versus 1.83 ± 0.05 L for continuous mixing. When the temperature was progressively decreased to 20°C, the same configuration was observed. The methane production was lowered from 76.2, 80.8, 67.5, 61.2 to 27.9, 35.8, 29.6 and 26.7% for 60, 45, 30 and 15 min/h, respectively. The mixing at 45 min/h showed a stable methane production as compared to all proposed mixing duration especially at psychrophilic temperature. The results would facilitate an empiric model that could help to establish more economical biogas reactor model. 
Key words: Hydrogenotrophic methanogens, mixing, H2/CO2 gas, bioconversion, temperature fluctuation.