African Journal of
Food Science

  • Abbreviation: Afr. J. Food Sci.
  • Language: English
  • ISSN: 1996-0794
  • DOI: 10.5897/AJFS
  • Start Year: 2007
  • Published Articles: 836

Full Length Research Paper

Improvement of the quality and shelf-life of minced beef mixed with soyprotein by Sage (Saliva officinalis)

Ali M. Ahmed1* and Takwa H. Ismail2
1Department of Food Hygiene and Control, Faculty of Vet. Med., Suez Canal University, Egypt. 2Department of Food Hygiene, Animal Health Research Institute, Ismailia Lab., Egypt.    
Email: [email protected]

  • Article Number - 1E96A7824479
  • Vol.4(6), pp. 330 - 334 , June 2010
  •  Accepted: 19 March 2010
  •  Published: 30 June 2010


The effect of various concentrations of sage (Saliva officinalis) on the microbial quality and the shelf-life of minced beef mixed with soybean protein was investigated. For this purpose, minced beef mixed with soybean protein samples were divided into four groups. First group was kept as a control group, others ones were treated with 0.1, 0.3 and 0.5% sage extract. All samples were stored at 4°C and microbiological evaluation was conducted at intervals of 0, 1, 3, 5 and 7 days post-storage for aerobic, anaerobic, enterobacteriaceae, yeast-mould, Bacillus cereus and Staphylococcus aureus counts. Minced beef pose a risk of food-borne infection or intoxication due to existence and growth of B. cereus and S. aureus. Sage extract concentration of 0.3 and 0.5%, significantly reduced aerobic, anaerobic, enterobacteriaceae, yeast-mould, B. cereus and S. aureus counts in the samples. Control and 0.1% sage groups did not comply with the Egyptian standards criteria for total aerobic counts when compared to other groups within the seven days of shelf-life. The results indicated that 0.3 and 0.5% sage extract improved the microbiological quality and prolonged the shelf-life of the minced beef to seven days of retail displayed at 4°C.


Key words: Minced beef, Sage, soybean, microbial quality and shelf-life.