February 2013
Construction the recombinant BCG targeting delivering IprI into macrophages: A new strategy of vaccine against tuberculosis
This study was aimed at constructing a recombinant Bacillus Calmette-Guérin (BCG) thatcan target the delivery of the intracellular pathogen resistance = 1 \* ROMAN I (Ipr1) gene into macrophages. To achieve this, a eukaryotic plasmid pBGOI was constructed, which co-expressed Ipr1 and green fluorescent protein (GFP). Then, pBGOI was transfected into murine macrophage cell line...
February 2013
Evaluation of medium components using Plackett-Burman design for phenol degradation by marine degrading Fusarium oxysporum
A new phenol-degrading marine fungal strain Fusarium oxysporum with high biodegradation activity and high tolerance of phenol was isolated from marine effluent of El-Max bay Alexandria, Egypt. Morphological, biochemical and 18S rRNA sequencing analysis identified the strain as F. oxysporum RA. This strain was capable of removing 12 and 8 mM of phenol and p-cresol in liquid minimal medium by...
February 2013
Characterization of CTX-M type extended spectrum β-lactamases of Escherichia coli isolated from urinary tract infections in Southern Turkey: The first report of CTX-M-14 producing Escherichia coli from Turkey
The aim of this study was to investigate the frequency of CTX-M type extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains which have become emerging etiologic agents of urinary tract infections (UTIs). A total of 310 E. coli isolates recovered from UTIs were screened for ESBL production by VITEK2 system and the double-disk synergy tests. In addition, ESBL positive isolates were...
February 2013
Changes in salicylic acid in grapevine treated with chitosan and BTH against Sphaceloma ampelinum, the causal agent of grapevine anthracnose
Chitosan and BTH selected as inducers of systemic resistance against Sphaceloma ampelinum, the causal agent of grapevine anthracnose, are presented for biological control of multiple pathogens causing either foliar or root diseases of various economic crops. In the present study, chitosan and BTH were investigated for their ability to induce some defense related chemicals that protect grapevine...
February 2013
Alteration of gut microflora through citric acid treated drinking water in preslaughter male broilers
This experiment was conducted to evaluate feed withdrawal and addition of citric acid in drinking water on the pH and microflora of gizzard, ceca and feces in preslaughter broiler chickens. A total of 24 male Ross 308 broiler chickens at the age of 42 days with almost equal weight were randomly divided into six treatments with four replicates each. Control group had free access to feed and water, but without...
February 2013
Epidemiology of methicillin resistant Staphylococcus aureus (MRSA) isolates from Pakistan
In order to have an understanding of the distribution of MRSA clones in Pakistan, where unregulated antibiotic use is widespread and the distribution of MRSA is thought to be high, an epidemiological study was designed. In this study, epidemiological relationships between 123 methicillin resistant Staphylococcus aureus strains, isolated between 2006 and 2008 from three tertiary care hospitals of Rawalpindi and...
February 2013
Acaricidal activity of Hypocrella raciborskii Zimm. (Hypocreales: Clavicipitaceae) crude extract and some pure compounds on Tetranychus urticae Koch (Acari: Tetranychidae)
The acaricidal activity of secondary metabolites from an entomopathogenic fungus,Hypocrella raciborskii Zimm. in controlling two-spotted spider mite, Tetranychus urticaeKoch (Acari: Tetranychidae) is explored in this study as a new agent for pest control. The crude extract was prepared in ethyl acetate. Secondary metabolites were elucidated using Nuclear Magnetic Resonance and High-Resolution Mass Spectrometry...
February 2013
Diversity of endophytic bacteria in the fruits of Coffea canephora
Endophytic bacteria colonize the internal tissues of plants without causing infection or negative effects on their hosts. This study investigates the occurrence and diversity of culturable endophytic bacteria in the fruits of Coffea canephora at three developmental stages. Isolation and quantification were performed in R2A culture medium, and the diversity was established using molecular methods and analysis...
February 2013
Characterization of microbial community in the selected Polish mineral soils after long term storage
The effect of 19-years storage period at air-dried condition (4°C) and impact of soils rewetting on microbial presence were studied. The topsoil (0 to 20 cm) of Mollic Gleysol,Eutric Cambisol, Rendzina Leptosol, Orthic Podzol and Eutric Fluvisol were used in the experiment. It was found that 10-days of soil incubation at full water capacity conditions and room temperature is enough for...
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