April 2018

Evaluation of gene xpert assay and microscopic examination for detection of Mycobacterium tuberculosis in sputum at Port Harcourt, Nigeria

The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant strains is of public health concern. The conventional Acid Fast Bacilli detection tool is weak in the current disease trend especially in children, patients with human immunodeficiency virus (HIV) and low bacterial load. The aim of this research was to assess effectiveness of the Gene Xpert MTB/RIF assay and Microscopy in...

Author(s): Confidence Kinikanwo Wachukwu, Stella Paulinus Okwelle, Easter Godwin Nwokah and Ollor Amba Ollor  

April 2018

Characterization of p30 membrane protein gene of Mycoplasma agalactiae isolates by polymerase chain reaction and restriction endonuclease enzyme assay

Seven hundred and eight (708) samples collected from the goats of Gujarat were screened for Mycoplasma agalactiae by culture and PCR using 16S rRNA based genus specific and species-specific primers. Amplification of the p30 membrane protein gene was carried out using specific primers and the resultant amplicons were subjected to restriction enzyme analysis. The isolates yielded 715 bp and 360 bp products with...

Author(s): Pranay K., Roy A. and Aher T. K.