January 2014
Microbiological evaluation of raw milk and coalho cheese commercialised in the semi-arid region of Pernambuco, Brazil
The aim of this study was to evaluate the microbiological quality of raw milk and coalho cheese commercialised in the Afrânio, Petrolina and Santa Maria da Boa Vista municipalities. The rural production of dairy products contributes significantly to the economy of these municipalities. We analysed 30 samples of raw milk and 30 samples of coalho cheeses. The microbiological quality was assessed by analysing total...
January 2014
Antimicrobial activity of methanolic extract and fractionated polysaccharide from Loligo duvauceli Orbingy 1848 and Doryteuthis sibogae Adam 1954 on human pathogenic microorganisms
In the present study, in vitro antimicrobial activity of crude methanolic extract of squid body tissue and fractionated gladius polysaccharide of two squid (Loligo duvauceli and Doryteuthis sibogae) from Cuddalore and Mudasalodai (Southeast Coast of India) landing centre was evaluated. The antimicrobial activity was screened against nine species of clinically isolated human pathogenic bacteria namely Bacillus...
January 2014
Spectrum of dermatophytoses in Jaipur, India
In order to determine the extent and causative agent of ringworm infections in Jaipur area, a study was done in outdoor patient Department of Skin, SMS Hospital, Jaipur. Out of 196 diagnosed cases, 148 were found to be positive by KOH examination while culture positive cases were 160. Trichophyton rubrum was the most common etiological type reported from 53 cases followed by Trichophyton mentagrophytes (23 cases)....
January 2014
Genetic characterization of H5N1 avian influenza viruses isolated from pet bird and chickens from live bird market in Bali and Bekasi (Indonesia), 2011
Indonesia has endemic status of H5N1 and the largest number of human cases of H5N1. Sources of human infections include mostly intensive contact with H5N1 virus or the environment contaminated by this virus. This study shows that feaces from pet bird victims and chickens were sold in live bird market (LBM) suspected to be the source of infection for human H5N1 in Bali and Bekasi in 2011. This study focused on the...
January 2014
Incidence rate of Staphylococcus aureus and Streptococcus agalactiae in subclinical mastitis at smallholder dairy cattle farms in Hawassa, Ethiopia
A longitudinal study was undertaken from December 2009 to February 2010 at Hawassa town, Ethiopia, in smallholder dairy farms to identify the role of Staphylococcus aureus and Streptococcus agalactiae in causation of subclinical mastitis and also to assess the role of selected risk factors in the transmission of these pathogens. A total of seven farms were selected. The farms were first screened for subclinical mastitis...
January 2014
Microbial dynamics as influenced by concentrate manure and inorganic fertilizer in alluvium soil of Varanasi, India
A field experiment was conducted during rabi season, 2009-2010 at Agricultural Research Farm, BHU, Varanasi, on alluvial soils to determine the effects of concentrate organic manure (wellgrow formulations) with levels of inorganic fertilizers. The results reveal that the higher soil microbial population was seen with the application of 100% NPK + 300 kg wellgrow soil ha-1. Soil enzymes varied with the production...
January 2014
Incidence of keratinophilic fungi in areas of Raipur City, Chhattisgarh region, India
In an endeavor to search for some wild isolates of keratinophilic fungi from the Raipur City of Chhattisgarh, 24 soil samples from different regions of the city were collected and screened using hair baiting technique. A total of six species which belong to two genera were isolated. They are Chrysosporium keratinophilum (27.78%), Chrysosporium pannicola (5.56%), Chrysosporium tropicum (13.89%), Chrysosporium sp. (close...
January 2014
Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)
Acinetobacter baumannii is an opportunistic pathogen responsible for nosocomial infections due to biofilm formation on the surface of implantable medical devices. Thirty (30) strains of A. baumannii were isolated from medical devices and tested for their ability to form a biofilm. The factors that may influence this process, such as the hydrophobicity of the bacterial wall, temperature, duration of implantation and the...
January 2014
Prevalence of antimicrobial resistant pathotypes of Escherichia coli in beef cattle and slaughterhouse premise
The study was undertaken to assess distribution of antimicrobial resistant and virulent genes in 72 Escherichia coli isolated from beef cattle and slaughter house. All the isolates were subjected for detection of virulent genes viz. stx1 and stx2 of shigatoxic E. coli (STEC), Lth of Enterotoxigenic E. coli (ETEC); antimicrobial resistant genes tetA, tetB, tetC for tetracycline and strA, strB for streptomycin by PCR...
January 2014
Antifungal agent production from a new marine Bacillus pumilusSMH101
Twenty two (22) marine bacterial strains were isolated and tested to inhibit some plant and human pathogenic fungi; Fusarium solani, Rhizoctonia solani, Aspergillus niger, Fusarium exosporium and Candida albicans. The most potent marine bacterium was identified as Bacillus pumilusSMH101 on using 16S rRNA. The peptone water medium (PW) showed maximum antifungal activity. The Placket-Burman experimental design was applied...
January 2014
Characterization of Lactobacillus strains isolated from Algerian children faeces for their probiotic properties
Lactic acid bacteria termed probiotics have preventive as well as curative effects on several types of diarrhoea of different aetiologies. The main objective of this study was to screen lactobacilli strains having probiotic traits, isolated from Algerian healthy children faeces on the purpose of using them further in children diarrheal illnesses. One hundred and twenty (120) lactic acid bacteria isolates were selected...
January 2014
Unique mutations existing in open reading frame 7 (ORF7) of non-porcine transmissible gastroenteritis virus (TGEV) strains isolated in China
Open reading frame (ORF7) of transmissible gastroenteritis virus (TGEV) plays important functions in regulating virus virulence. In this study, the ORF7s were cloned from non-porcine TGEV isolates (HYM-09, HYM-09-1 and HYM-09-2), respectively. Sequence analyses showed that there existed some new unique mutations in the three non-porcine TGEV ORF7s, and which had relative farther genetic relationship and evolution...
January 2014
Assessment of the microbiological quality of koozh, a fermented millet beverage
Koozh is a fermented beverage made with millet flour and rice, and consumed by ethnic communities in Tamil Nadu, India. Six street vended samples were assessed for the total bacterial count (TBC), lactic acid bacteria (LAB) count, yeast-mould count (YMC), coliforms at 35°C and pathogens. The koozh pH ranged from 4.3 to 4.9 with high titratable acidity. Although no Staphaylococcus sp. and Listeria sp. were found,...
January 2014
Detection of extended spectrum beta lactamases in typhoidal salmonellae by phenotypic methods
Extended spectrum beta lactamase (ESBL) producing members of the family Enterobacteriaceae are a major health problem in the hospitals and community. These enzymes confer bacterial resistance to penicillins, first, second and third generation cephalosporins and aztreonam. The prevalence of ESBLs varies from organism to organism and is increasing day by day. The study was designed to test typhoidal salmonellae for...
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