December 2014
Detection of differentially expressed growth dependent noncoding RNAs in Sulfolobus solfataricus
Noncoding RNA mediated gene regulation exists in all the three kingdoms of life including prokaryotes, eukaryotes and archaea. In prokaryotes, the ncRNAs typically bind to the 5’ end of the messenger RNAs to facilitate its activation or repression. The repression of gene expression in eukaryotes by ncRNAs is regulated by its binding to the 3’-untranslated region. A significant amount of information is...
December 2014
Management of sugarcane smut (Ustilago scitaminea) with fungicides and bio-agents
The efficacy of nine fungicides with different concentrations was tested against Ustilago scitaminea, using spore germination inhibition technique. Tilt and Emisan (50 µg/ml) are proved to be the most effective against the spore germination of U. scitaminea, and they completely inhibit it. These are followed Score with 100 µg/ml after 24 h of incubation. Vitavax, Dithane M-45 and Antracol (200 µg/ml)...
December 2014
Effect of some culture extracts of Aspergillus oryzae on dehulling properties of pigeon pea (Cajanus cajan L.)
Pre-dehulling treatments with some culture extracts of Aspergillus oryzae of various incubation periods played an important role in improving the dehulling properties of pigeon pea (Cajanus cajan L.). Yield percent of dehulled grains and dehulling efficiency increased concurrently with increase of incubation period of culture extracts. Maximum dehulled grains were achieved by 12-day old culture extract to the tune of...
December 2014
Disruption of negative regulators (SP_nsdA and SP_nsdB) in Streptomyces peucetius causes doxorubicin overproduction
Pathway-specific regulatory genes generally participate in the secondary metabolites-related biosynthesis process. The genes nsdA and nsdB were reported to have a negative effect on the production of actinorhodin, prodigiosin and calcium-dependent antibiotic from Streptomyces coelicolor. In this study, we searched for similar genes in the Streptomyces peucetius genome, the doxorubicin producer. Amino acid sequence...
December 2014
Analysis of arsenic species in realgar bioleaching solution by capillary zone electrophoresis
Realgar was bioleached by Acidithiobacillus ferrooxidans BY-3. A capillary zone electrophoresis (CZE) method was developed to analyze arsenic species in realgar bioleaching solution. This way can simultaneously separate arsenic compounds including arsenite (iAsIII), arsenate (iAsV), monomethylarsenic (MMAV) and dimethylarsenic acid (DMAV). Borate and cetyltrimethylammonium bromide (CTAB) were selected to compose a...
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