African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 18 April; 4(8)

April 2010

Optimizing the conditions of α- amylase by an Esturian strain of Aspergillus spp

  Water samples and sediments were collected from waterways in between two back water lakes namely Vambanad and Asthamudi, in Alukadavu, Kollam along the west coast of Kerala, India. 20 fungal species were isolated and identified by lacto phenol cotton blue staining method. From this, Aspergillus flavus was selected for starch hydrolysis Agar Medium for amylase activity under submerged fermentation...

Author(s):   Arun Sasi, Manthiri Kani, A. Panneerselvam, G. Jegadeesh, K. Muthu and M Ravi Kumar  

April 2010

Investigation of different infectious pathways used by Streptococcus suis type 2 and other bacteria in mice

  The purpose of this study was to investigate the infectious pathways used byStreptococcus suis type 2 and enterohemorrhagic Escherchia coli O26 (EHEC O26) in mice. Six-week mice were intraperitoneally injected with mitomycin (0.2 mg/mouse) to decrease the protective immunity. The mice were then challenged with S. suis type 2 and EHEC O26 strains through wounded skin, stomach lavaging...

Author(s): Xingjian Cao, Zhaoming cao, Guohua Tao, Wei Xie, Xiang Chen and Shuo Wang

April 2010

Fingerprinting of repetitive DNA sequences in the genus Anabaena using PCR- based techniques

  In this study, ten species of Anabaena were used to test the congruence between the traditional morphological classification system and the present molecular classification system. The electrophoretic patterns for 10 cyanobacterial strains belonging to the genera (Anabaena) were used for molecular analysis using the repeated PCR technique. Genetic diversity was assessed using the banding patterns of...

Author(s): Anbalagan Ezhilarasi and Narayanaswamy Anand

April 2010

Preliminary in-vitro antibacterial activities of ethanolic extracts of Ficus sycomorus Linn. and Ficus platyphylla Del. (Moraceae)

  Antibacterial therapeutic failure due to emergence of resistant bacterial strain is a world wide phenomenon. The search for effective antibacterial substances from sources such as plants has become a necessity to overcome emergent of bacterial resistant in clinical practice. The dried leaves and stem barks of Ficus sycomorusand Ficus platyphylla were collected in Samaru-Zaria, Nigeria in July...

Author(s): Adeshina Gbonjubola Olusesan, Okeke Claire-Lorentz Ebele, Osuagwu Nonyelum Onwuegbuchulam and Ehinmidu Joseph Olorunmolaa

April 2010

The effect of cultivation conditions on the mycelial growth of a dark-septate endophytic isolate

  The EF-37 isolate, one of DSE fungi, is beneficial to the growth and development of its host plant, Saussurea involucrata Kar. et Kir. The cultivation requirements including basic culture medium, temperature, light, pH, carbon source and nitrogen compounds were studied for their effects on mycelial growth of a dark-septate endophytic (DSE) fungus EF-37 by using one-factor-at-a-time method. Potato...

Author(s): Ya-li Lv, Li-hua Sun, Fu-sheng Zhang, Yue Zhao and Shun-xing Guo

April 2010

Antimicrobial activity of various extracts of Centaurea cankiriense A. Duran and H. Duman

  The antimicrobial activity was determined using the single disc diffusion method. The hexane, methanol and ethyl acetate extracts were assessed for antimicrobial activity against 13 bacteria and a yeast-like fungus, Candida albicans. While flower extracts of Centaurea cankiriense showed significant antibacterial activity against tested strains, the susceptibility of the test microorganisms was...

Author(s): Arzu Cansaran, Nazime Mercan DoÄŸan, Mehtap Öztekin and Gülümser Acar

April 2010

The validity of the diagnostic methods in predicting pulmonary tuberculosis

  In our study, we aimed to determine the validity of diagnostic methods for tuberculosis. Eighty-one people suspected to have tuberculosis were included in the study. The validity of the applied methods for the diagnosis of tuberculosis tuberculin skin test (TST), sputum smear, and used in diagnostic chest X-ray findings (CXR), clinical features and history were evaluated as culture was considered the...

Author(s): Sulhattin Arslan, Levent Özdemir, Yeltekin Demirel and Ibrahim Akkurt

April 2010

Uptake of textile dye Reactive Black-5 by Penicillium chrysogenum MT-6 isolated from cement-contaminated soil

  The present study focused on the decolorization of textile dye Reactive Black-5 by actively growing mycelium of fungus Penicillium chrysogenum MT-6, which was isolated from the cement-contaminated soil. Dye decolorization was probably associated with fungal growth and hyphal uptake mechanism (Biosorption/Bioadsorption). Dye uptake was strongly depended on mycelial...

Author(s): Serkan Erdal and Mesut Taskin

April 2010

Antibacterial activity and mechanism of recombinant human α- defensin 5 against clinical antibiotic-resistant strains

  The increasing clinical bacterial strains resistant to conventional antibiotics have being a great challenge to the public’s health. As a novel kind of antimicrobial agent, defensins are undoubtedly worthy of exploitation for the treatment of antibiotic-resistant bacteria. To evaluate the antibacterial potency of recombinant mature human α-defensin 5 (rmHD5) against clinical pathogenic strains, we...

Author(s): A. P. Wang, Y. P. Su, S. Wang, M. Q. Shen, F. Chen, M. Chen, X. Z. Ran, T. M. Chen and J. P. Wang

April 2010

Effects of Enterobiasis on primary school children

  The aim of the present study was to investigate the possible adverse effects ofEnterobiasis vermicularis infection on primary school children in Sivas, Turkey, to know whether it affects their school success, mean weight and height or not. Seven primary schools in the central region of Sivas were included in the present study. A total number of 3659 questionnaires were completed by class...

Author(s): Ali Çeliksöz, Mehmet Aciöz, Serpil DeÄŸerli, A. Yasemin Öztop and Ahmet Alim

April 2010

Antibiotic resistance microbes in tropical mangrove sediments in east coast peninsular, Malaysia

  The study has been conducted at Tanjung Lumpur, mangrove swamp on January 2009 to isolate and identify the bacterial community in mangrove soil and their resistance against antibiotics. Identified bacteria were Aeromonas hydrophilagroup 1 and 2, Escherichia coli 1, Chryseomonas luteola, Chromobacterium violaceum, Pseudomonas aeruginosa, Serratia rubudaea, Klebsiella...

Author(s):   K. C. A. Jalal, Nur Fatin U.T,  Mardiana M.A, Akbar John B, Kamaruzzaman Y. B, Shahbudin S, and Muhammad Nor Omar   

April 2010

Diagnostic criteria in Crimean Congo haemorrhagic fever disease and cost analysis

  In recent years, Crimean Congo haemorrhagic fever disease (CCHF) has created a serious health problem in our country. The disease takes place among haemorrhagic viral diseases. This study was made with CCHF diagnosed patients accepted in emergency services to determine the diagnostic criteria and cost effectivness for CCHF. CCHF patients who were accepted in our emergency service between 2004 and 2008 were...

Author(s): Sevki Hakan Eren, Ä°lhan Korkmaz, Fatma Mutlu Kukul Güven, Can Aktas and Didem Ay

April 2010

Phenotypic and molecular characterization of SHV, TEM, CTX-M and extended-spectrum β-lactamase produced by Escherichia coli, Acinobacter baumannii and Klebsiella isolates in a Turkish hospital

  A total of 94 clinical isolates were collected from Gazi University Hospital, Turkey.Presence of ESBL positivity was detected using the double disk synergy test (DDST). ESBL isolates were further typed for the blaTEM, blaSHV, blaCTX-M andblaOXA using designed primers. ESBLs were found in 65 (69.14%) isolates using DDST. Plasmid DNAs of potentially ESBL positive strains...

Author(s): Elif Burcu Bali, Leyla Açık and Nedim Sultan

April 2010

Oral colonization and boric acid susceptibility of yeast in boron mineral workers

  In this study, we aimed to investigate the effects of boron on in vivo oral yeast colonization in study groups which are exposed to boron dust in different sections of the boron mine. The study was carried out in the boron mining areas of two districts (Eskisehir and Balikesir) of Turkey. We included 184 people working in open quarry and stone milling unit, 144 people working in the factory and 150...

Author(s):   Zafer Cetinkaya, Semsettin Karaca, Mustafa Kulac, Ihsan Hakki Ciftci, Gulsah Asık, Osman Cenet and Nuri Kiraz  

April 2010

Isolation and characterization of plum-seeds degrading aerobic bacteria from plum-grove soil

  The aim of this study was to isolate plum-seeds degrading bacterium from plum-grove soil. Some bacteria utilizing plum-seeds were isolated from the plum-grove soil samples by biodegradation assay. Among them, five bacteria had the activity of cellulase and xylanase. Four of the strains grew in the medium containing xylan instead of glucose. Partial sequencing of 16S rRNA and genome indicated that all the...

Author(s): Haruka Kurishita, Hitomi Yoshida, Namika Yamaji, Naoko Okumura, Asuka Kirimura, Satoru Matsuda and Hiroshi Ueno