January 2010

Application of methylene blue dye reduction test (MBRT) to determine growth and death rates of microorganisms

  An efficacy of a compound for antimicrobial characteristics is typically evaluated through experiments to obtain the minimum inhibitory concentration (MIC). Similarly, a minimum activating concentration (MAC) can be obtained for a compound necessary for growth. We report a protocol for the determination of MIC and MAC for antibiotic and exhibition assays, respectively using methylene blue reduction...

Author(s): Subir Kumar Nandy and K. V. Venkatesh

January 2010

Lack of antimicrobial activities of Dicranopteris linearis extracts and fractions

  The aim of the present study is to determine the antimicrobial activity of the aqueous, chloroform and methanol extracts of the leaves of Dicranopteris linearis(Gleicheniaceae) using the micro-broth dilution method. The leaves of D. lineariswere soaked separately in distilled water (dH2O), chloroform and methanol in the ratio of 1:20 (w/v) for 72 h at room temperature, while some part of the...

Author(s):   Z. A. Zakaria, A. Mat Desa, K. Ramasamy, N. Ahmat, A. S. Mohamad, D. A. Israf and M. R. Sulaiman

January 2010

ISSR for comparison of cross-inoculation potential of Colletotrichum capsici causing chilli anthracnose

  Thirty-four isolates of Colletotrichum spp. including 2 species, Colletotrichumgloeosporioides and Colletotrichum capsici, from anthracnose on Bell pepper, Long cayenne pepper and Bird’s eye chilli were isolated and their pathogenicity was proven via fruit inoculation. Pathogenicity tests divided pathogenic potential into low, medium and high virulence groups. It is clearly...

Author(s): Kanchalika Ratanacherdchai, Hong-Kai Wang, Fu-Cheng Lin and Kasem Soytong

January 2010

Effect of Helicobacter pylori infection on the lipid, lipoproteins, apolipoprotein-A1, lipoprotein (a) and apolipoprotein-B in patients with gastritis

  There are evidences of relationship between chronic infections of Helicobacter pylori(H. pylori) and gastritis in some previous studies. The gastritis caused by H. pylorileads to a change in serum levels of lipids, lipoproteins, apolipoprotein-A1 (Apo-A1), apolipoprotein-B (Apo-B) and lipoprotein-a (Lp(a)). These changes will lead to thrombogenesis and other cardiovascular diseases. The aim of this...

Author(s): Mohammad Hassan Khadem Ansari, Mir-davood Omrani, Biet Sayyah and Sina Khadem Ansari

January 2010

Prevalence and diagnostic aspects of sputum smear positive tuberculosis cases at a tertiary care institution in Rwanda

  Approximately one third of the world’s population is infected with Mycobacterium tuberculosis and 9.27 million new cases of TB occurred in 2007. Developing countries disproportionately shoulder the global burden of disease with the highest estimated rates in the world, with an...

Author(s): Claude Mambo Muvunyi, Florence Masaisa, Claude Bayingana, Andre Musemakweri, Leon Mutesa and Teresa Carbonell Hernandez,

January 2010

Prevalence of antibodies to infectious bronchitis virus (IBV) in chickens in southwestern Nigeria

  A total of 672 sera from apparently healthy commercial and indigenous chickens of different ages were screened for antibodies to infectious bronchitis (IB) virus using the enzyme-linked immunosorbent assay (ELISA). Samples obtained from breeder, layer, grower and indigenous chicken flocks in Oyo, Ogun, Ondo and Lagos states of southwestern Nigeria were screened. The total seroprevalence was 82.7% with ELISA...

Author(s): B. O. Emikpe, O. G. Ohore, M. Olujonwo and S. O. Akpavie

January 2010

Microbiological quality of packaged drinking water brands marketed in Ibadan metropolis and Ile-Ife city in South Western Nigeria

  Continuous increase in the sale and indiscriminate consumption of packaged drinking waters in Nigeria is of public health significance. One hundred and eight samples comprising 16 bottled and 20 sachet water brands purchased randomly all over Ibadan and Ile-Ife cities in South Western Nigeria were analysed for presence of bacterial indicators of water quality. Total heterotrophic bacteria plate counts (HPC)...

Author(s): O. Oyedeji, P. O. Olutiola and M. A. Moninuola

January 2010

Patients with suspected visceral leishmaniasis in Istanbul

  We performed a four year study to investigate the Visceral Leishmaniasis (VL) cases in, Turkey. Fifty-nine patients with suspected VL from Istanbul were included in this work. Bone marrow and blood samples of these patients were tested for possible VL infection using several methods including serological tests, microscopy, PCR. Nineteen (32.2%) patients had positive results for VL after one or more of the...

Author(s): Huseyin Cakan, Suat Saribas, Vecdet Oz, Erdal Polat, Mustafa Aslan and Bekir Kocazeybek

January 2010

Association of IGF-1 gene polymorphism with milk production traits and paternal genetic trends in Iranian Holstein bulls

  The effects of Insulin-Like Growth Factor-1 polymorphism were examined on milk production traits and genetic trends in the Iranian Holstein bulls. A total of 282 bulls were genotyped for SnaBI restriction site in the 5' flanking region of IGF-1 by applying PCR-RFLP method. The allele frequencies for C and T alleles were 0.562 and 0.438, respectively. The results indicated that...

Author(s): Yousef Mehmannavaz, Cyrus Amirinia, Mortaza Bonyadi and Rasoul Vaez Torshizi

January 2010

Periodic changes in Prosopis cineraria associated AM population at different soil depth and its relationship with organic carbon and soil moisture

  Arbuscular mycorrhizal  fungi (AMF) spore population was studied at eight levels of soil depth under two different sites viz., Prosopis cineraria under agro forestry area (with moong bean) and P. cineraria as sole crop at four different quarters of the year (July, October, January, April). In agro forestry areas the spore population recorded higher as compared to P....

Author(s):   Neelam Verma, J. C. Tarafdar and K. K. Srivastava