June 2013
Proteolytic inventory of Thermococcus kodakaraensis
Proteolysis is a very crucial process for development and survival of the cell. Genome sequence data can be employed to estimate proteolysis inventories of different organisms. In this review, we exploit genome sequence data of hyperthermophilic archaeon Thermococcus kodakaraensis to have an overview of the proteolysis in this microorganism. The overview is based on those peptidases that have been...
June 2013
Anti-aflatoxigenic effect of essential oils on Aspergillus Spp. isolated from pistachio in Saudi Arabia
Aspergillus spp. associated with pistachio from the regions of Riyadh, Dammam and Abha were isolated. The most frequently isolated Aspergillus spp. came from Dammam (56.1%), followed by Riyadh (47.6%), and the least frequently isolated species were from Abha (26.2%). Aspergillus flavus showed the highest prevalence in the investigated samples. Three different culture media were...
June 2013
A novel assay combined PCR with junction probe for rapid detection of anaerobes DNA
Herein, we developed a novel assay for detection of anaerobes by polymerase chain reaction (PCR) combined with JP (junction probe). The PCR products were processed by bead-based extraction to get single strand DNA which could be used in the junction probe reaction. The experiment results demonstrate that the new assay which we call PCR-JP could detect at least 10 copies of DNA with high sensitivity and specificity...
June 2013
Antifungal and antibacterial properties of surfactin isolated from Bacillus subtilis growing on molasses
The aim of the study was to evaluate antifungal and antibacterial properties of surfactin isolated from Bacillus subtilis growing on molasses. Molasses replaced the traditional microbiological media to culture the B. subtilis. 10 phytopathogens and 30 E. coli strains were used in the study. The results demonstrated the ability of surfactin produced byBacillus sp. growing on molasses to...
June 2013
Biocides susceptibility pattern and phenotypic detection of Efflux pump in Staphylococcus aureus isolates from two tertiary hospitals of Pakistan
This study was conducted on wound isolates, collected both from in-patients and out-patients of two tertiary hospitals of Peshawar, Pakistan. A total of 70 Staphylococcus aureus isolates, including 35 methicillin resistant Staphylococcus aureus (MRSA) and 35 methicillin sensitive Staphylococcus aureus (MSSA) were tested using different biocides and antibiotics. The biocides used in this study were Cetrimide,...
June 2013
Antimicrobial potential of various fractions of Thuja orientalis
The present study was conducted to evaluate the antimicrobial activity of various extracts (viz. methanol, acetone and ethyl acetate) of Thuja orientalis and its preliminary phytochemical screening. In vitro antimicrobial activity was tested by agar well diffusion assay against human pathogenic microbes which are Gram positive (viz. S. aureus andB. subtilis), Gram negative bacteria...
June 2013
The retrieval of antibiotic sensitivity by 2-phenoxyethanol adapted-multi drug resistant (MDR) clinical isolate of Pseudomonas aeruginosa
2-Phenoxyethanol (2-PE) is a biocide used as a preservative in pharmaceutical, cosmetic and perfumery formulations. It is known for its rapid bactericidal effect against a wide range of bacteria. In the present study, clinical samples of multi drug resistant (MDR)Pseudomonas aeruginosa and Escherichia coli were grown in an increasing sub-minimal inhibitory concentration of 2-PE to observe if adaptation...
June 2013
Surveillance and molecular analysis of pandemic A (H1N1) virus in Shandong province of China during the 2009 to 2010 season
The objective of the study was to analyze the epidemiologic, demographic and molecular characteris-tics of pandemic A (H1N1) viruses (A (H1N1) pdm 09) in Shandong Province between May, 2009 and March, 2010. Nasopharyngeal swabs from influenza-like-illness samples were collected and diagnosed by Real Time RT-PCR method. The positive samples were incubated and cultured in MDCK cells. HA and NA genes of isolated A (H1N1)...
June 2013
Bioactive potential of a new strain of Streptomyces sp. PP14 isolated from Canadian soil
A new actinomycete strain designated PP14 was isolated from a Canadian soil sample, by a dilution agar plating method using a chitin-vitamins B medium supplemented with different antibiotics as selective agents. This actinomycete produced antimicrobial substances and exhibited strong antifungal activity against mycotoxigenic fungi includingAspergillus carbonarius (M333) and Penicillium expansum, and also...
June 2013
A new semi-selective agar medium for recovery and enumeration of antagonistic yeast, Pichia guilliermondii strain Z1 from orange fruit surface
The aim of this work was to develop a semi-selective medium for recovery and enumeration of Pichia guilliermondii strain Z1, a reliable biocontrol agent against postharvest pathogens of citrus fruit, and to assess the population dynamic of this antagonistic yeast on orange fruit in relation to incubation temperature and time of incubation. PDA is the basal medium used in this study which allows the...
June 2013
Effect of different pH values on growth and sporulation of Estye vermicola
Esteya vermicola, an endoparasitic fungus of pinewood nematode, exhibits great potential as a biological agent against pinewood nematodes. In order to improve the growth rate, sporulation and proportion of lunate conidia, the optimization of pH value for E. vermicolawas tested. The diameter and dry mass of E. vermicola in PDA medium were increased greatly at pH 5 after 15 days, while the diameter and dry...
June 2013
Identification and detection of Salmonella strains isolated from chicken carcasses and environmental sources in Dourados, MS, Brazil
The application of methodologies that can rapidly and precisely identify Salmonella in both foods and water samples is of great interest to public health. Salmonella sp. was isolated from chicken carcasses and water samples from aquaculture tanks using both traditional culture methods and bacteriological analyses. DNAs from these isolates were extracted and Multiplex Polymerase Chain Reactions...
June 2013
Effect of different pH values on growth and sporulation of Estye vermicola
Esteya vermicola, an endoparasitic fungus of pinewood nematode, exhibits great potential as a biological agent against pinewood nematodes. In order to improve the growth rate, sporulation and proportion of lunate conidia, the optimization of pH value for E. vermicolawas tested. The diameter and dry mass of E. vermicola in PDA medium were increased greatly at pH 5 after 15 days, while the diameter and dry...
June 2013
Fourteen (14) cases of acute focal bacterial nephritis in children
To investigate clinical features, laboratory, and imaging characters in Chinese children, we conducted a retrospective study on 14 children with acute focal bacterial nephritis (AFBN) admitted in Beijing Children’s Hospital during January 2005 to December 2009. Six girls and eight boys with a mean age of 4.7 years (range: 0.3-11.4 years) were followed up on average 1.7 years (range: 0.5-3.5 years). Leukocyturia...
June 2013
Physico-chemical and biological factors in the distribution of cyanobacteria population in three different sampling sites of South India
The current work was involved in the distributional analysis of the cyanobacteria strains present in the predominant water outlets present in the city, for analysing the types of strains found during the different seasonal interims. The cyanobacteria distribution in water from two different stations of Southern India were analysed at four months interval during the period of July 2002 to June 2004. The...
June 2013
Immobilization of cellulase on modified mesoporous silica shows improved thermal stability and reusability
Cellulase was covalently immobilized using modified mesoporous silica as carrier, and the characters of immobilized cellulase were investigated including the optimum pH, the optimum temperature, thermal stability, reusability, storage stability and enzyme kinetics. As compared to the free enzyme, the optimum pH of the immobilized cellulase showed no changes, the optimum temperature showed a slight increase to 60°C....
June 2013
Identification of extended-spectrum-beta-lactamases(ESBLs), metallo-beta-lactamases (MBLs), Amp-C and KPC ß-lactamases among Klebsiella pneumoniae isolated from adults and pediatric patients in Iran
Klebsiella pneumoniae is one of the major bacteria that cause acute infections. β-lactamases are main defensive mechanisms in bacteria against drugs. The aim of this study was to determine antibiotic resistance patterns and detection of extended-spectrum-beta-lactamase (ESBL), MBL, Amp-C and KPC β-lactamases among K. pneumoniae strains isolated from adults and infants. This descriptive study was...
June 2013
In vitro inhibition of aminoglycosides-resistant Staphylococcus aureus by modifying enzyme inhibitory protein of Pseudomonas aeruginosa
Eighty clinical bacterial isolates were collected from Sayed Galal hospital in Cairo. Minimum inhibitory concentrations (MICs) were determined for five aminoglycoside antibiotics: netilmycin, sissomycin, spectinomycin, gentamicin and neomycin. The most potent gentamicin-resistant bacterial isolate (IS-20) was identified as Staphylococcus aureus. On the other hand, the same eighty clinical bacterial isolates were...
June 2013
Detection of Chlamydophila pneumoniae IgG in blood samples taken from coronary sinus during bypass surgery
Chronic infection may promote arterial inflammation, thereby contributing to the initiation or progression of atherosclerosis. Chlamydophila pneumoniae is an obligate intracellular human respiratory pathogen. It has been suggested that coronary atherosclerosis may be an autoimmune process triggered by an infectious agent, most likely C. pneumoniae. The purpose of the present study was to investigate the...
June 2013
Estimation of the Salmonella spp. prevalence in pig farms with dry and wet feeding
Human salmonellosis originating from pork is an important zoonotic disease, and the objective of this study was to determine whether the Salmonella shedding was lower for pigs herds provided wet-feed compared to those on traditional dry rations. Four wet-feeding farms and six dry-feeding farms were selected. Individual faecal and feed samples were collected (faces 30, feed 10 from pigs per farm), and analysed...
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