May 2011
Anti- tuberculosis activities of medicinal plants used in the treatment of tuberculosis in HIV patients in Nigeria
The risk of infection in burnt patients is high and at the present the antibiotic resistance of bacteria isolated from these patients has increased. Therefore, the aim of this study was detection of antibiotic resistance pattern of Escherichia coli, Staphylococcus aereus andPseudomonas aeruginosa isolated from burnt patients to some current antibiotics. The results showed that all of isolates...
May 2011
Production and characterization of cellulolytic enzymes by Pleurotus florida
Three Pleurotus spp., Pleurotus florida, Pleurotus ostreatus and Pleurotus sajar-caju, were screened for cellulolytic enzyme production under submerged fermentation conditions. Of these, P. florida, was studied for optimizing medium composition, incubation period, initial pH and incubation temperature to maximize cellulolytic enzyme production. Malt extract at...
May 2011
Surface display of multi-epitopes synthetic gene of Mycobacterium tuberculosis on Salmonella typhi Ty21a using a pgsA anchor protein
We have developed a recombinant Salmonella typhi Ty21a vaccine candidate (rSTvacIII) displaying a multi-epitopes mycobacterial antigen called the VacIII (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoS1, Hsp 16.3 and MTB 8.4 genes) on the surface of S. typhi Ty21a (Ty21a) using PgsA protein as a carrier. The expression of VacIII protein on the surface of...
May 2011
Optimization of bacteriocin production by Lactobacillus plantarum YJG, isolated from the mucosa of the gut of healthy chickens
The objective was to develop an optimal, albeit low-cost medium for bacteriocin production, thereby facilitating industrial production. Soybean meal and peptide, two low-cost nitrogen sources often applied in industrial fermentation, were used to replace their expensive counterparts in De Man Rogosa and Sharpe (MRS). Two factors were first chosen from the 11 considered in the Plankett-Burman (PB) design....
May 2011
Isolation and characterization of a cold-active amylase from marine Wangia sp. C52
Amylase activity was detected in the culture medium of marine Wangia sp. C52, which was isolated from the Southern Okinawa Trough deep-sea sediment. In the present study, a cold-active amylase was purified to homogeneity from the culture broth by ammonium sulfate precipitation and gel filtration chromatography. The molecular mass of the amyalse was 58 kDa, and its isoelectric...
May 2011
Comparison of different detoxification methods for corn cob hemicelluose hydrolysate to improve ethanol production by Candida shehatae ACCC 20335
Corn cob hydrolysis with 1% (v/v) of sulfuric acid yielded 36.49 total reducing sugars (g/l) along with various fermentation inhibitors such as fufural, phenolics compounds and acetic acid. The acid hydrolysate detoxified with overliming plus activated charcoal brought about a maximum decrease in fufural (100%), acetic acid (62.4%) and phenolics compounds (96.6%). Treatment of hydrolysate with overliming...
May 2011
Pathogenic and molecular characterization of Pythium species inducing root rot symptoms of common bean in Rwanda
A series of 231 samples of bean plants affected by bean root rot were collected from different areas of Rwanda in order to characterize the causal agents. The collected samples were used to isolate 96 typical Pythium colonies which were classified into 16Pythium species according to their respective molecular sequences of the ribosomal ITS fragments. Inoculation assays carried out on a set of...
May 2011
Phtyochemical screening and comparative study of antimicrobial activity of Aloe vera various extracts
The phytochemical screening and comparative study of antimicrobial activity of Aloe veraextracts was carried out. The phytochemical screening revealed the following metabolites: saponins, alkaloids, glycosides, tannins, protein, and flavonoids. While cardiac glyocosides and steroids were absent. The antimicrobial activities of the gel in 10% DMSO, methanol and aqueous extracts of green rind and leaf pulp...
May 2011
Comparison of cELISA and IFA tests in the serodiagnosis of anaplasmosis in cattle
This study was performed to compare indirect fluorescence antibody (IFA) test with competitive ELISA (cELISA) for detection of antibodies to Anaplasma marginale in cattle. In this study, a total of 484 cattle were examined on farms that had a positive history of anaplasmosis. Thin blood smears were prepared from each examined cattle and the cELISA and IFA tests were performed. Samples in which...
May 2011
A laboratory assessment of the potential of the entomopathogenic fungi Beauveria bassiana (Beauvarin®) to control Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) and Sitophilus granarius (L.) (Coleoptera: Curculionidae)
This research was carried out to evaluate the suppressive efficacy of entomopathogenic fungi Beauveria bassiana against adults of Callosobruchus maculatus (F.) and Sitophilusgranarius (L.) on stored grains in darkness (27±2°C and 65±5% RH). The insects were inoculated by following immersion in five aqueous suspensions with concentrations determined with...
May 2011
Production of recombinant cellulase enzyme from Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)
Successful utilization of Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)mushroom as a type of edible locally isolated mushroom in Egypt at the Mushroom Research Center (Mubarak City for Scientific Research and Technology Applications), to produce extensive hydrolyzing cellulase complex enzymes. This hydrolysis was approached in submerged culture supplemented with avicel PH101 as a...
May 2011
Bacteriophage specific to nisin producing-Lactococcus lactis subsp. lactis TFF221, a starter culture inThai fermented food
A lytic bacteriophage fTFF221, specific for nisin-producing Lactococcus lactis subsp.lactis TFF221, was first isolated from kung-jom, a Thai traditional fermented shrimp paste. The plaques were clear and round-shaped on the lawn of bacteria, indicating its lytic activity. Restriction analysis indicated that genome of phage fTFF221 was double-stranded DNA. Transmission electron...
May 2011
Alteration of intercellular adhesion molecule-1 (ICAM-1) expression in the liver and serum level of HBV DNA in chronic hepatitis B patients after interferon treatment
To investigate the intercellular adhesion molecule-1 (ICAM-1) expression in the liver and serum hepatitis B virus (HBV) DNA level in chronic hepatitis B (CHB) patients before and after interferon (IFN) treatment, a total of 51 CHB patients were recruited and treated with IFN-α 1b for 6 months. The expressions of ICAM-1 and serum HBV DNA were determined at baseline and after 6-month treatment...
May 2011
Value of direct antigen detection methods in diagnosing women with suspected genital Chlamydia trachomatis infection
Establishing a method that would allow the quick and cost- effective diagnosis ofChlamydia trachomatis (C. trachomatis) is of highest interest. We aimed to evaluate the diagnostic efficacy of direct antigen detection methods [direct fluorescent antigen detection (DFA), Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR)] in comparison to culture method, to...
May 2011
Serological survey of brucellosis in livestock animals in Sarab City (East Azarbayjan province), Iran
Brucellosis is an infectious and zoonotic disease of worldwide distribution. Despite its control program, the disease is endemic in Iran and remains one of the most important public health problems. The aim of this survey was to determine the seroprovalence of brucellosis in livestock animals in Sarab City, Iran. A total of 1500 animals (600 cattle, 740 sheep and 160 goats) were...
May 2011
Vaginal lactobacillus distributions and functions in pregnant women
Lactobacillus plays an important role in keeping the vaginal micro-ecological balance and inhibiting the growth and reproduction of pathogens. However, the knowledge ofLactobacillus distributions and functions in pregnant women remains unclear. The study was undertaken the prevalence of Lactobacillus and the specific strains of Lactobacilluspresent in vaginal discharge from women at...
May 2011
Antifungal metabolites from Phomopsis sp. By254, an endophytic fungus in Gossypium hirsutum
Chemical and bioassay-guided fractionation of the chloroform-methanol (1:1) extract ofPhomopsis sp. By254, an endophytic fungus residing in the root of Gossypium hirsutum, yielded three compounds. The structures of these compounds were elucidated by a combination of spectroscopic analysis (MS, 1D- and 2D-NMR) to be epoxycytochalasin H (1), cytochalasin N (2), and cytochalasin H (3). All the...
May 2011
Molecular and seroepidemiological study of Leishmania infantum infection among humans, dogs and wild canines from Azarshahr (new endemic focus), Iran
An epidemiological survey of visceral leishmaniosis among domestic and wild canines as well people was carried out in the new endemic focus of Azarshahr (northwest of Iran) to assess the distribution of the disease and the possible association between infection in dogs and people during 2008-2009. At first using direct agglutination test (DAT), an examination of visceral leishmaniosis was carried out among...
May 2011
Serological survey of HDV-Ab in HBsAg positive blood donors in Shahrekord, Iran
Hepatitis delta virus (HDV) is a defective ssRNA virus requiring the provision of hepatitis B virus (HBV) for packaging of new HDV virions. Since the epidemiological features of HDV in this part of Iran seem to be unknown, the aim of this research was to determine the seroprevalence of HDV in HBsAg positive blood donors, in Shahrekord, Iran. In this research, we announce the detection of HDV-Ab from...
May 2011
Isolation and characterization of microsatellite loci from the Chinese white wax scale Ericerus pela Chavannes (Homopetera: Coccidae)
Eight microsatellite loci were isolated using an enrichment protocol from Ericerus pelaChavannes, representing the first microsatellite loci available for the white wax scale. All loci were polymorphic within E. pela. Allele numbers range from six to eleven with the observed heterozygosity (HO) ranging from 0.0000 to 1.0000. These primers screened in this study provide a foundation for population...
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