September 2010
Production, purification and characterisation of extracellular L-asparaginase from a soil isolate of Bacillus sp.
L-asparaginase is an anti-neoplastic agent used in lymphoblastic leukaemia chemotherapy. Soil microbial isolates were screened for potential producers of L-asparaginase using a phenol red indicator growth medium and the microbe producing the largest hydrolysis zone was selected. The isolate was characterised by biochemical tests and was found to belong to Bacillus sp. The enzyme production...
September 2010
Diagnosis of infectious Hematopoietic Necrosis in Rainbow Trout Hatcheries, Iran
Distribution of infectious hematopoietic necrosis (IHN) was studied in rainbow trout fish farms in Chaharmahal and Bakhtyari Province which is a major trout culture region in Iran. Overall 150 suspected rainbow trout fry with less than 3 g body weight from 30 fish farms in Chaharmahal and Bakhtyari Province, Iran, were studied and fishes of 10 hatcheries (33.3% of all studied farms) found to be infected with...
September 2010
Antibacterial activity of propolis and honey against Staphylococcus aureus and Escherichia coli
The disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and gradient-plate techniques were implemented to evaluate the antibacterial activity of honey and propolis against Staphylococcus aureusand Escherichia coli. The growth of S. aureus was inhibited by application of propolis and honey at concentrations of 2.74 to 5.48 mg...
September 2010
Prevalence and antimicrobial resistance patterns of methicillin-resistant staphylococci (MRS) isolated in a Veterinary Teaching Hospital in Brazil
We investigated the prevalence of methicillin-resistant staphylococci (MRS) in humans and dogs and evaluated the antimicrobial resistance patterns of these bacteria at a Veterinary Teaching Hospital. Specimens from 50 human subjects and 50 dogs were studied. Isolates were identified by Gram-staining, biochemical reactivity and resistance to antimicrobials. While no isolates of methicillin-resistantStaphylococcus...
September 2010
Identification of the CagM's location in cytomembrane of Helicobacter pylori
Helicobacter pylori infect nearly half of the world’s population and are associated with a spectrum of gastric maladies. The cytotoxin-associated gene pathogenicity island (cag PAI) encoding a type IV secretion system (T4SS) has been implicated in a series of host responses during infection. The cag PAI contains about 28 - 30 open-reading frames, for most of which the exact function...
September 2010
Isolation and serotyping of Legionella pneumophila from Goreangab Dam and hostel shower heads in Namibia
This is the first report of Legionella pneumophila serotypes in Namibia. Using modified buffered charcoal extract agar selective medium, L. pneumophila was isolated from Goreangab dam water and University of Namibia (UNAM) student hostel shower heads. Latex agglutination tests showed that L. pneumophila serotypes 1 - 15 were present in Goreangab dam water and serotypes 2 - 15...
September 2010
Survival analysis to determine the length of latent period of Mycosphaerella pinodes on peas (Pisum sativum L.)
Blight caused by Mycosphaerella pinodes is one of the most devastating diseases of pea that causes yield losses of over 50% in some years and may cause total failure to the crop under epidemic conditions. During this study, a sensitive disease assessment and statistical tool was developed for Mycosphaerella blight on peas, the latent period was used to discriminate between different...
September 2010
Prevalence of tuberculosis and human immunodeficiency virus (TB/HIV) co-infections amongst patients with bronchopulmonary disorders in Lagos
Tuberculosis (TB) still remains a global public health problem of enormous dimension. This study was carried out between March 2007 and June 2008 to determine the prevalence of TB and TB/HIV co-infections amongst patients with bronchopulmonary disorders in Lagos State, Nigeria. 1280 patients were screened for acid-fast bacilli (AFB) by microscopy and culture at the TB reference laboratory of the Nigerian...
September 2010
The effect of fructo-oligosaccharides on blood RBC count and digestive enzyme activities of oxyeleotris lineolatus
This study was designed to evaluate the effect of fructo-oligosaccharides (FOS) on blood RBC (red blood cell) count and digestive enzyme activities of oxyeleotris lineolatus. 60 oxyeleotris lineolatus were randomly divided into three groups with 20 fish in each group: control, low and high dose of FOS-treated groups. Animals in the control group consumed basic feed, animals in FOS-treated groups consumed the...
September 2010
Arbuscular mycorrhizas and dark septate endophytes colonization status in medicinal plant Lycium barbarum L. in arid Northwestern China
Lycium barbarum L. is an oriental medicinal plant. The arbuscular mycorrhizal (AM) and dark septate endophytic (DSE) fungal associations of this plant species are completely unknown. In this study, the AM and DSE fungal colonization status in three L. barbarumcultivars in arid Northwestern China were investigated. The results showed that the three cultivars were simultaneously colonized...
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