July 2013
Isolation and characterization of thermostable protease producing Bacillus pumilus from thermal spring in Jordan
A protease-producing thermophilic bacterium designated Bacillus pumilus JT3 was isolated and identified from a hot spring in Jordan by biochemical tests. The growth and enzyme production in shake flask culture using different operational conditions were studied and observed in a relatively wide temperature range from 25 to 80°C and pH from 6.5 to 13, with an optimal temperature of 60°C and...
July 2013
Some applications of α-amylase produced by Bacillus subtilis NCTC-10400 and Bacillus cereus ATCC 14579 under solid state fermentation
This paper describes the production of α-amylase by Bacillus subtilis NCTC-10400 andBacillus cereus ATCC 14579 using wheat bran as a substrate under Solid State Fermentation (SSF). Supplementation of carbon and nitrogen sources resulted in marginal increase in α-amylase production. Highest production was observed with fructose and ammonium chloride (180.8 U/g) for B....
July 2013
Dissection of antimycotic and antitumor effect of honey bee venom in- vitro and vivo
Candida albicans is generally one of the most commonly isolated fungal pathogen from human body. Bee venom (BV) is a natural substance, reported to have antimicrobial activity and to boost the immune system. This study aimed to evaluate the effect of bee venom as antimycotic agent and to asses its antidimorphic effect in comparison with some antimycotic drugs available in the market, and also,...
July 2013
A comparison of Neo-Sensitabsâ„¢ tablets and paper discs in disc diffusion antimicrobial susceptibility testing
A comparative evaluation of Neo-SensiitabsTM (Rosco, Taastrup, Denmark) and BBL paper discs (Becton Dickinson, Cockeysville, MD) according to the CLSI Performance Standards for Antimicrobial Susceptibility Testing was done. A total of 303 clinical isolates were included: 60 Enterobacteriaceae (including ESBLs isolates), 63 Pseudomonas aeruginosa, 63 Staphylococcus aureus,...
July 2013
Effect of the methanol extracts of Salvia libanotica, Rosmarinus officinalis, Capparis spinosa and Achillea fragrantissima against two strains of Staphylococcus aureus
Control of Staphylococcal infections is difficult, especially ecreasing the incidence of methicillin-resistant Staphylococcus aureus (MRSA) among hospitalized patients. Meanwhile, medicinal plants have many traditional claims including the treatment of ailments of infectious origin. Four Jordanian medicinal plants, Salvia libanotica, Rosmarinus officinalis, Capparis spinosa,...
July 2013
Effects of different carbon and nitrogen sources in Broth culture on the growth of Rhizobium leguminosarum bv. phaseoli and symbiotic effectiveness of haricot bean (Phaseoulus vulgaris L.) in Eastern Hararghe soils of Ethiopia
An experiment was conducted to test the growth efficiency of Rhizobium leguminosarum bv. phaseoli strain no. 46 on different nutrient media in laboratory and consecutively test the performance of the Phaseoli on nitrogen fixation and growth performance of the bean. Various treatment combinations were made for the Rhizobium growth study by replacing mannitol with sucrose, starch, and molasses as...
July 2013
Screening out of coliform bacteria from different location of Gomti river in Lucknow
The presence of feacal coliform indicates the presence of feacal material from warm-blooded animals such as Escherichia coli or Klebsiella pneumonae. Their presence can be taken as an indication of the potential danger of health risks those feacal possess. Members of two bacteria groups, coliforms and feacal streptococci are used as indicators of possible sewage contamination...
July 2013
Improvement in in vitro growth rates of Ganoderma species with industrial wood waste supplements
The bracket-like polypore fungi of the genus Ganoderma, as a pathogen of oil palm (Elaeis guineensis Jacq.), is a major concern primarily because it plays a significant role in the economy of many countries in South-East-Asia. Growth of several Ganoderma isolates was examined on various culture media to develop a medium for rapid growth. Growth analyses revealed that growth of the...
July 2013
A new subspecies of Lentinellus and its phylogenetic relationship based on ITS sequence
Lentinellus castoreus is a complex group. A new taxon under this species was found in northeast of China in this study. The new one was distinguished from the other sub-taxon by its morphological characteristics of denty tomentum, subdistant lamellae and inamyloid hyphae. The assignment of the new taxon was supported by phylogenetic analyses based on the internal transcribed spacer (ITS)...
July 2013
The physiological state of Lactoccocus lactis and Propionibacterium freudenreichii strains in co-cultures induced by low temperature and osmotic stress
The bacterial physiological state, type of interactions and changes in metabolism ofLactococcus lactis and Propionibacterium freudenreichii strains in co-culture were studied in skimmed milk in response to osmotic [3% (w/v) NaCl] and low temperature (10°C) stress during long-term incubation. Changes in the integrity of cell membrane were examined by LIVE/DEAD® BacLight™...
July 2013
In vitro antibacterial and antifungal effect of some medicinal plants
The aim of this study was to evaluate the antimicrobial activity of five species of plants known as medicinal plants and used commonly in folkloric medicine. They are: Nigella sativa, Foeniculum vulgare, Black piper, Loranthus capitellatus, Cassia alata and Drynariaqurshiforia. In this study, we investigated the antibacterial and antifungal...
July 2013
Antimicrobial activity of indigenous wildly growing plants: Potential source of green antibiotics
Antibiotics are an important class of pharmacological agents used for treating infections, a major cause of human morbidity and mortality. Although antibiotics were first isolated from fungi and bacteria but over the years more and more synthetic antibiotics are flowing in market. During the last two decades, the development of drug resistance as well as the appearance of undesirable side effects of...
July 2013
Evaluation of in vitro antagonism and protection against enteropathogenic experimental challenge of different strains of Bifidobacterium
Gastrointestinal microflora highly impacts their host mainly by performing a great variety of metabolic activities, protecting the host from pathogenic colonization. Mother's milk is a prebiotic factor which stimulates bifidobacteria growth in vivo. All strains of bifidobacteria were isolated on MRS medium (in addition to 0.05% cystéine HCL and 2 mg/l of nalidixic...
July 2013
Effects of a functionalized ionic liquid on the growth and antioxidant enzymes of Synechococcus sp. PCC7942
Cyanobacteria are prokaryotic algae found in oceans and freshwaters worldwide. These organisms are important primary producers in aquatic ecosystems because they can provide essential food for grazers and herbivores. In this study, the effects of a functionalized ionic liquid [1-(2’-hydroxylethyl)-3-methylimidazolium chloride (HOEtMIMCl)] on growth and antioxidant enzymes of a freshwater...
July 2013
Antifungal effects of essential oils of three medicinal plants on post-harvest rot of Valencia oranges at normal and storage temperatures
Post-harvest rots are the main limiting factors in storing horticultural products. Currently, control of postharvest citrus diseases relies mainly on synthetic fungicides. Biologicalcontrol using naturally occurring substances has been recently explored for managing postharvest decay of citrus fruit. Essential oils of three medicinal plants (Ocimum basilicum, Mentha...
July 2013
Antimicrobial activity of three wines against Campylobacter jejuni and the effect of low temperature on their survival ability
Forty-three (43) Campylobacter jejuni isolated from sheep feces and carcass swab samples by conventional culture method were 100% confirmed by polymerase chain reaction (PCR) test. The nucleotide sequences of 362 bp showed 100% homology to the completed genome of C. jejuni subsp. M1 and 81116. Antimicrobial property of different types of wine against C. jejuni was investigated....
July 2013
Identification and genotyping fingerprinting of 2,4-DAPG producing Pseudomonas spp.
PCR based DNA finger printings were performed by ISSR primers, gene specific primers (Plt) and primers derived from 16S ribosomal RNA. Fifteen primers pairs were used to detect the polymorphism between 45 isolates of Pseudomonas spp. Results revealed that clear polymorphism and similarity coefficient ranged from 0.00 to 0.44 with ISSR primers, 0.01 to 0.83 with gene specific primers and 0.22 to 1.00...
July 2013
Dietzia species as a cause of mastitis: Isolation and identification of five cases from dairy cattle
Many infective agents are implicated as causal agents of mastitis. Miscellaneous causes of mastitis in bovine due to actinomycetes such as nocardiae have been described but neither Rhodococcus nor Dietzia species have been reported. The aim of this study was to report the isolation and characterization of rhodococcus-like actinomycetes as causal agents of mastitis in cattle....
July 2013
Enhanced soybean biomass by co-inoculation of Bradyrhizobium japonicum and plant growth promoting rhizobacteria and its effects on microbial community structures
Co-inoculation of rhizobia with plant growth promoting rhizobacteria (PGPR) plays an important role in both promotion of nodulation and plant growth of leguminous plants. In this study, rhizobacteria were screened for co-inoculation with Bradyrhizobium japonicumon soybean for their capacity to promote the nodulation under aseptic condition. The obtained rhizobacteria were further screened in soybean-nodulating...
July 2013
Identification of three related genera, Salmonella, Citrobacter and Proteus using API 20E, 16S-23S rDNA intergenic transcribed spacer fingerprinting and 16S rDNA sequencing
A total of 176 presumptive Salmonella strains isolated from different sources (food, environment, veterinary, and human) were analyzed by API 20E, serotyping, ITSF/ITSR-PCR and MINf/MINr-PCR. On the other hand, the 16S-23S rRNA intergenic transcribed spacer region was used to aid in their identification. Test results were confirmed by 16S rRNA sequencing. Of 176 suspected S....
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