March 2013
Decolorization of methyl red by an isolated Pseudomonas putida strain MR1
The dye methyl red was completely decolorized at 24 h of incubation by Pseudomonas putida MR1 isolated from the dyestuff contaminated soil, collected from the textile industrial area of Sanganer, Jaipur, Rajasthan. Maximum decolorization was achieved when the isolate was incubated at 34°C and pH 7. The decolorization was confirmed by studying the spectral analysis of the dye. 16S rRNA partial gene...
March 2013
Food borne bacteria isolated from spices and fate of Cronobacter sakazakii ATCC 29544 in black pepper exposed to drying and various temperature conditions
Food-borne disease outbreaks caused by spices have been on the increase in the recent years, although they are rarely reported in the developing countries. The purpose of this work therefore, is to show that spices sold in selected retail outlets in Durban South Africa may contain unacceptable level of pathogenic microorganisms including Cronobacter sakazakii which may survive desiccation and high temperature....
March 2013
Interaction between soil textural components, flavonoids in the roots and mycorrhizal colonization in Juniperus procera in Saudi Arabia
The degree of infection of Juniperus procera roots by arbuscular mycorrhiza fungi (AMF) is highly indicative of the response of plants to environmental stress and is affected by the presence of flavonoids in the roots. However, relatively little is known about the relationship between soil textural components (sand, clay, silt content) and the percentage of AMF in the field soil. The aim of this work was to...
March 2013
Antibacterial activity of Lactic acid bacteria isolated from Tunisian camel milk
The present study describes the composition of raw camel milk from south Tunisia in comparison with bovine milk and investigate the antimicrobial activity of isolated Lactic acid bacteria (LAB). The physical analysis of milk showed that camel milk was more acidic and viscous than cow milk while chemical composition showed that bovine milkwas richer in fats (40 ± 3.4 g/l) and dry matter...
March 2013
Red sea bream iridovirus infection in marble goby (Bleeker, Oxyeleotris marmoratus) in Taiwan
An outbreak of infectious disease in 30,000 marble gobies (Bleeker, Oxyeleotris marmoratus) cultured at a density of 5,000 to 8,000 fish per pond at a fish farm in the central Taiwan was reported to the Nantou County Animal Disease Center and the Central Taiwan Aquatic Disease Center. The mortality rate was approximately 40% (12,000/30,000). In May 2010, clinical signs of sluggish behavior, atypical swimming...
March 2013
Diversity and antimicrobial activity of isolated endophytic bacteria from Deodeok (Codonopsis lanceolata) of different locations and ages
Deodeok (Codonopsis lanceolata) were collected from two mountainous areas (Jirisan and Yarongsan) in Korea. Endophytic bacteria were isolated from two, five, and seven-year-old deodeok (Codonopsis lanceolata) roots. The bacterial diversity of deodeok root samples was evaluated by 16S rDNA analysis. In the roots collected from Jirisan, we identified the presence of Bacillus...
March 2013
Aetiology, antimicrobial susceptibility and predictors of urinary tract infection among febrile under-fives at Muhimbili National Hospital, Dar es Salaam-Tanzania
Urinary tract infection (UTI) is a common cause of fever in children and contributes to morbidity and mortality. This study aimed at determining prevalence, aetiology and antimicrobial susceptibility pattern of the isolates at Muhimbili National Hospital (MNH), Dar es Salaam- Tanzania. Demographic data were collected using a pretested questionnaire. 382 febrile children below five years admitted in the general...
March 2013
Construction of a yeast two-hybrid cDNA library from second-generation merozoites of Eimeria tenella
To construct a yeast two-hybrid cDNA library from second-generation merozoites ofEimeria tenella (EtsMZ), total RNA of EtsMZ was extracted using Trizol reagent and mRNAs were isolated from total RNA. The first-strand cDNAs were synthesized by reverse transcription using MMLV. The dscDNAs acquired by Long-distance Polymerase Chain Reaction (LD-PCR) and purified by Chroma Spin TE-400 Column were...
March 2013
Sero and media culture prevalence of Salmonellosis in local and exotic chicken, Debre Zeit, Ethiopia
The main constraints to the development of indigenous chicken production in rural Ethiopia includes diseases, inadequate feed, predator, poor housing system and to a lesser extent limitation on financial and knowledge on management. In Ethiopia, the current rates of mortality due to diseases from day old to adult chicken are estimated to be 20-50%. Among the diseases: Fowl typhoid and pullorum disease are mentioned to...
March 2013
Differential interaction between isolates of Rhizoctonia solani AG-3 and potato cultivars
Twenty fungal isolates of Rhizoctonia solani AG-3 sclerotia were isolated from diseased potato tuber samples that were obtained from potato-producing areas in the Lower Egyptian governorates. The pathogenicity of the 20 isolates of R. solani AG-3 was tested on nine potato cultivars under greenhouse conditions. The black scurf disease incidence (BSDI) and stem canker index (SCI) were used as the...
March 2013
Physico-chemical aspects during the composting of municipal solid wastes and sewage sludge in a semi-industrial composting plant
This study investigated at first the main physico-chemical and biochemical parameters during the co-composting process of municipal solid waste and sewage sludge under Tunisian pedo-climatic conditions, and secondly to assess potential toxic elements (PTEs) content of the finished compost and to evaluate its biological stability and maturity. Results show that the temperature revealed three classical steps, the...
March 2013
In vitro antagonistic efficacy of plant extracts on the enzyme activity of Colletotrichum gloeosporioides
Anthracnose fungus, Colletotrichum gloeosporioides was isolated in culture from the diseased fruit samples of chilli (Capsicum annuum). The fungus was identified by morphological and molecular analysis. Plant root extracts of A. precatorius and R. tetraphylla and chemical fungicide, dithane-M45 were tested for their inhibitory effects on the cell wall degrading enzymes of C....
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