August 2018

Low accuracy of the McFarland method for estimation of bacterial populations

The McFarland method is designed to estimate bacterial concentrations by means of a turbidity scale (absorbance) which consists of a series of tubes previously calibrated, and with an optical density produced by the precipitation of barium sulphate. This absorbance is compared to bacterial populations. The most used absorbance is the one corresponding to 0.5 on that scale, which assumes a population of 1.5×108...

Author(s): Lozano Guzmán Eduardo, Beatriz Santos Ramirez, Cervantes Flores Maribel, María Guadalupe Nieto Pescador and Francisco Javier Moreno Cruz  

August 2018

Efficacy of plasma micro broth dilution assay for antifungal susceptibility testing of Candida albicans

Fungal infection and antifungal drug resistance especially in the treatment of immuno suppressive syndromes, has created an increased demand for reliable and affordable methods of in vitro testing of antifungal agents that can assist in their diagnosis. This study aimed to investigate antifungal susceptibility testing method that is reliable, flexible, affordable, accurate, cheap and less time consuming in order to...

Author(s): Fon E. F., Oko A. O., Chia P. N. and Yongabi K. A.  

August 2018

Antimicrobial susceptibility pattern of Gram negative bacteria isolated from intensive care units in Al-Ahsa, Kingdom of Saudi Arabia

Antimicrobial resistance by bacteria isolates continues to receive attention globally. This investigation looks into the antibiotic susceptibility pattern of Gram negative bacteria isolated from intensive care unit patients in Al-Ahsa, KSA. Bacteria samples were classified based on the CDC criteria for the definition of ICU infections. Gram negative bacteria had been isolated on MacConkey agar using basic...

Author(s): Lorina Badger-Emeka, Abdulrahman Abdulhadi Al-Sultan, Abdullatif Sami Alrashed, Mohammed Sami Alhaddad and Afnan Khalifah Al-Barjas  

August 2018

Isolation and identification of Escherichia coli, Salmonella and Pasteurella from holding grounds of live-bird markets at Addis Ababa, Ethiopia

A cross-sectional study was conducted to isolate and identify of Escherchia coli, Pastuerella multocida, Salmonella gallinarum and Salmonella pullorum from the holding grounds of five purposively selected Addis Ababa live bird markets from November 2016 to May 2017 using bacterial culture, grams staining and biochemical testing. A total of 90 pooled fecal samples were collected as both deep (35) (bottom layer of the...

Author(s): Surra Gebeyehu, Dereje Tulu and Chaluma Negera