August 2012
Stereoselective nitrile hydratase
Nitrile hydratase, catalyzing the hydration reaction of nitrile to amides, is an important enzyme which has been used in the industrial production. Various studies on nitrile hydratase revealed its crystal structure and also proposed some hypothesis of its catalytic mechanism. Recent reports suggested the stereoselectivity of nitrile hydratase, which is regarded as a potential character to enrich its application....
August 2012
Biochemical screening of chocolate spot disease on faba bean caused by Botrytis fabae
In this work, three faba bean cultivars were studied. The cultivar Giza 429 was the most susceptible cultivar to chocolate spot disease, followed by Giza 40, which was moderately susceptible; cultivar Giza 461 was found to be resistant. Four fungal species associated with diseased leaves were isolated from naturally infected samples and identified asBotrytis fabae, Alternaria...
August 2012
Optimization of the fermentation medium for Paecilomyces tenuipes N45 using statistical approach
In the present study, a sequential statistical approach was applied to optimize the medium in submerged cultivation of Paecilomyces tenuipes N45. Desirability value was used as response to simultaneously enhance the yields of mycelium, adenosine, polysaccharide and cordyceps acid. Based on single-factor optimization strategy, the suitable carbon sources, nitrogen sources and inorganic salts were obtained....
August 2012
Optimal conditions for growth and magnetosome formation of Acidithiobacillus ferrooxidans
Magnetotactic bacteria (MTB), which contain nano-scale membrane bound crystals of magnetic iron minerals, are of great interest in microbiology, biomineralization, advanced magnetic materials and bio-geosciences. However, no application has been exploited on a commercial scale due to the fastidious lifestyle of most cultured MTB. Acidithiobacillus ferrooxidans is a facultatively aerobic bacterium which can...
August 2012
The community structure of microbial in arable soil under different long-term fertilization regimes in the Loess Plateau of China
To explore the long-term impact of different fertilization regimes on microbial communities, we targeted total bacteria and the genus Pseudomonas at a field site established in 1979, in the Loess Plateau of China. Soils were sampled thrice during the growing period of winter wheat in 2008, with treatments: N (inorganic N), NP (inorganic N and P), MNP (farmyard manure, inorganic N and P), M (farmyard manure),...
August 2012
Light intensity dependent expression of Lhca gene family encoding LHCI in Dunaliella salina
In order to compare the expression pattern and function of Lhca gene families, theDsLhca genes have been cloned, and two novel genes have been isolated by screening aDunaliella salina cDNA library with a DNA probe coding for the conserved first transmembrane helix of this protein. The number of exons in the different DsLhca genes was slightly conserved. The deduced pre-proteins of the two...
August 2012
Antimicrobial activities and chemical properties of Tamarindus indica L . leaves extract
Tamarinds indica L. of the family Fabaceae is known to be used in folk medicine. Among efforts to verify this scientifically, the antimicrobial activities of the leaves extract were investigated against Gram negative and positive bacteria. The results were supported by SM images. The phytochemical constituents of the dried powdered leaves were extracted using aqueous and organic solvents. The antimicrobial activity...
August 2012
Alteration of outer membrane proteins, secreted proteins and virulence gene expression of Salmonella enterica serovar Typhimurium in response to long-term starvation
The foodborne pathogen Salmonella enterica serovar Typhimurium was subjected to starvation in seawater microcosms for three years to study modifications in its outer membrane and extracellular protein profiles. After incubation, outer membrane proteins and extracellular proteins profiles of stressed bacteria were found to be altered when analyzed by sodium dodecyl sulfate-polyacrylamide gel...
August 2012
The influence of food preservation methods on Escherichia coli, Salmonella enteritidis and Pseudomonas aeruginosa lipopolysaccharide composition and liberation
Escherichia coli, Salmonella enteritidis and Pseudomonas aeruginosa were subjected to pasteurization, ultra high temperature (UHT) treatment and sodium benzoate preservation to determine the effect of these treatments on lipopolysaccharide (LPS) structure. S. enteritidis was the only bacterium that showed an overall decrease in LPS liberation after subjection to the heat...
August 2012
Genotyping of Mycobacterium tuberculosis complex based on restriction enzyme analysis of the hsp65 gene
Several genotypes of Mycobacterium with varied degree of virulence and drug resistance exist globally. Grouping of the genotypes into principal genetic groups is critical for tuberculosis (TB) control. This study determined the suitability of polymerase chain reaction- restriction enzyme analysis (PCR-REA) technique as an epidemiological typing tool and a technique for drug resistance surveillance of TB....
August 2012
Ectomycorrhizal community structure in chronosequences of Pinus densiflora in eastern China
Ectomycorrhizal (ECM) fungal communities of Pinus densiflora were studied along a chronosequence. Four age classes were sampled: 5, 30, 55 and 80 year-old. Morphotyping (anatomical and morphological identification) and molecular analysis (internal transcribed spacer region amplification and sequencing) were used to identify ECM root tips. A total of 43 ECM morphotypes were distinguished, 38 of which were...
August 2012
Iron overload in chronic Hepatitis C patients with Candida albicans infection
Patients with chronic hepatitis C (CHC) often have increased liver iron. The distribution of pathogenic microorganisms including Candida albicans in human body mainly depend up on the concentration of iron which changes during hepatitis C virus (HCV) infection. This study aimed to measure iron overload and its correlation to C. albicans infection in patients with CHC. According to the presence...
August 2012
Study on the identification and bio-corrosion behavior of one anaerobic thermophilic bacterium isolated from Dagang oil field
A slightly curved rod strictly anaerobic, thermophilic bacterium, was isolated from Dagang oil field, China. Its fundamental physiology and corrosion characteristic were analysed. The strain BF4 was Gram-positive. The growth occurred at 45-78°C (optimum was 60°C) and pH 4.5-9.5 (optimum was 7). Substrates could be used involving D-glucose, fructose, sucrose, raffinose, mannose, lactose, lactate, and cellobiose....
August 2012
Presence and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus in raw and pasteurized milk and ice cream in Tabriz by culture and PCR techniques
Reports of methicillin-resistant Staphylococcus aureus (MRSA) in food have become more frequent in recent years. The aim of this study was to estimate the presence of MRSA in raw milk, pasteurized milk and ice cream in Tabriz by culture and polymerase chain reaction (PCR) techniques, as well as the determination of their antimicrobial susceptibility. For this purpose, 300 samples of raw milk, pasteurized...
August 2012
Study the prevalence of TT virus infection in South Iranian volunteer blood donors
Exposure to blood transfusion was associated with increase in the risk of transfusion transmitted (TT) viral infection and this led to the hypothesis that TT virus may be related to undefined post-transfusion hepatitis. Therefore in this study the prevalence of TT virus DNA in volunteer blood donors in relation to Alanine transaminase (ALT) and aspartateaminotransferase (AST) levels were...
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