September 2011
Utilization of kitchen waste for the production of green thermoplastic polyhydroxybutyrate (PHB) by Cupriavidus necator CCGUG 52238
Polyhydroxybutyrate (PHB) was produced by Cupriavidus necator CCGUG 52238 using organic acids from fermented kitchen waste. HPLC and nuclear magnetic resonance (NMR) analyses revealed that the acid comprised mainly of lactic and acetic acids. In shake flask culture, the lactic acid concentration above 10 g/L inhibited both cell growth and polyhydroxybutyrate (PHB) production. The PHB production by the strain...
September 2011
Prevalence of extended spectrum beta lactamases (ESBL) in clinical isolates from a teaching hospital in Peshawar, Pakistan
Infections caused by ESBL producing members of the enterobacteriaceae have rapidly increased all over the world. ESBL increase the possibility of failure of empiric antimicrobial regimens. The aim of this study was to determine the prevalence of ESBL in bacterial isolates and to look into the options for treating infections caused by these organisms. A total of 4,150 isolates of enterobacteriaceae were studied. ESBL...
September 2011
Effect of irrigation water on the incidence of Salmonella spp. on lettuces produced by urban agriculture and sold on the markets in Dakar, Senegal
The aim of our survey was to assess the effect of irrigation water of the microbiological quality on the production chain of lettuce in the Dakar area. Microbiological analysis showed that 35% of irrigation water was contaminated by Salmonella spp. between the two water-types used for irrigation (groundwater and wastewater), no significant difference (p>0.05) in their degree of contamination was found. The...
September 2011
The relationship and diagnostic value of C-reactive protein (CRP) and C-reactive protein (hsCRP) for myocardial infarction
Atherosclerosis is the main cause of myocardial infarction (MI), inflammation is considered as a main cause of atherosclerosis. Inflammatory indicators such as C-reactive protein (CRP) are considered as a diagnostic marker for MI in recent years. We studied the relationship between seropositivity to CRP and high-sensitivity- reactive protein (hsCRP) with MI and compared their relationship and...
September 2011
Nano-modeling of insulin-like growth factor 1 (IGF-1) by computational methods
The empirical force fields have great difficulty in simulating folding of insulin-like growth factor 1 (IGF-1). In an effort to understand the conformational preferences that may be attributed to stereoelectronic effects, a number of computational studies are carried out. Monte Carlo, molecular dynamics and Langevin simulation methods by MM+, amber andoptimized potential for liquid simulations (OPLS) force fields...
September 2011
Helicobacter pylori and CagAantibodies in hyperemesis gravidarum (HG)
In order to determine whether infection with Helicobacter pylori and CagA strain is associated with hyperemesis gravidarum (HG), a study was conducted in Al- Zahra obstetrics and gynecology hospital in Tabriz, Iran between May 2007 and February 2008. Forty-four (44) pregnant women with the diagnosis of HG and forty-four (44) normal pregnant women of matched gestational age were included in this prospective...
September 2011
Antibacterial activity of plant extracts against oral and skin pathogens
The aim of this study is to examine the antibacterial activity of plant extracts. Both broth dilution and disc diffusion methods were used to assess the antibacterial activity of these extracts against skin and oral pathogens. Eryngium caucaseum Trautv, Eryngium bungeiBoiss and Adiantum capillus-veneris have shown antibacterial activity againstStreptococcus pyogenes, Streptococcus...
September 2011
Analysis of biochemical divergence for classification of six native silkworm based on total cholesterol, triglycerides, high density lipoproteins (HDL) cholesterol, low density lipoproteins (LDL) cholesterol, total lipase and lipids
This research studies a comparison between the levels and the abundance total cholesterol, triglycerides, HDL cholesterol (High Density Lipoproteins), LDL cholesterol (Low Density Lipoproteins), total lipase and lipids in six native groups of silkworm. These six biochemical parameters and enzymes play important roles in the silk production, digestion and other physiological activities. Other aim of this experiment was...
September 2011
Phenotypic characterization and enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) of Aeromonas spp. and Vibrio spp. strains isolated from Sparus aurata fish farm (Khenis, Tunisia)
This study characterises the Aeromonas and Vibrio strains isolated from seawater and sediments out of Sparus aurata marine farm. The bacterial strains were isolated on Thiosulfate-Citrate-Bile-Sucrose (TCBS) agar plates and described using different bacteriological tests and using standardised micro-methods “API 20 NE strips”. The exoenzymes production and antibiotics...
August 2011
Microbiological analysis and screening of lactic acid bacteria from Tunisian date palm sap
Date Palm sap is a fresh juice called “Legmi” widely produced and consumed in Southern Tunisia. The aim of this research was to study microbiological group’s distribution present in 10 palm sap samples, and to select Lactic acid bacteria (LAB) with suitable properties for use as starter cultures in sap lactic fermentation. Microbiological analysis showed high load in fresh palm sap. Mesophilic...
September 2011
Genotypic distribution of rotavirus strains causing severe gastroenteritis in children under 5 years old in Borazjan, Iran
Rotaviruses are recognized as the most common causes of severe gastroenteritis and death among children worldwide. The aim of this study was to evaluate the disease burden of rotavirus gastroenteritis and the prevalence of different G genotypes of rotaviruses circulating in children aged <5 years old who were hospitalized for acute gastroenteritis in Borazjan City, Iran. This cross sectional-descriptive study was...
September 2011
Assessing the potential of Tetradenia riparia in treatment of common skin conditions in rural communities of South Africa
Tetradenia riparia has been used by traditional healers for treatment against various ailments including wound healing and skin sores. The scientific validation of the use of T. riparia against selected strains of Gram positive and Gram negative bacteria as well as selected fungal strains has substantiated the use by traditional healers. The relatively high inhibitory concentration for both bacterial and...
September 2011
Antimicrobial susceptibility of Clostridium difficile isolated from different sources of Imam Reza Hospital, Tabriz
The aim of this study was to determine antibiotic susceptibility of Clostridium difficile isolated from different sources. Two hundred stool samples of staff, patients (at admission to the wards and the same patients after seven days of hospitalization), and 135 samples of hospital environment were collected. Three standard methods including direct plating onto cycloserine-cefoxitin fructose agar, alcohol...
September 2011
Cytotoxicity and antimicrobial activity of Teucrium scordium L. (Lamiaceae) extracts
Cytotoxicity and antimicrobial activity of cyclohexane, dichlormethane and methanol extracts of Teucrium scordium subspec. scordioides was studied. Cyclohexane and dichlormethane extracts of T. scordium possessed high citotoxicity against MDA-MB-361 cells (IC50=130.33±0.1 μg/ml and IC50=189.89±3.99 μg/ml, respectively). Dichlormethane extract was more effective against...
September 2011
Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from patients of lower respiratory tract infection
The aim of this study was to determine the antibiotic susceptibility pattern of Pseudomonas aeruginosa from sputum samples of lower respiratory tract infection (LRTI) patients admitted to the Department of Pediatrics and Pulmonary Medicine and also to update clinicians on various antimicrobial alternatives available in treatments. Sputum samples from 298 patients were cultured identified and antibiotic sensitivity was...
September 2011
Characterization and optimization of agarase from an estuarine Bacillus subtilis
A bacterium producing agarase was isolated from the vellar estuary and potential strain was identified as Bacillus subtilis. The ideal parameters was found to be of pH 8, temperature of 40°C and 3% of NaCl concentration for optimal growth and agarase activity. Agar as carbon source and yeast extract as nitrogen source was found to be suitable for optimum growth and agarase activity. The activity of enzyme...
September 2011
Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital
The aim of this study is to investigate the genetic backgrounds of methicillin susceptibleStaphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA) and heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) isolates isolated from clinical specimens of the patients with verified infections in a Chinese teaching hospital. Macro E test (MET) was used to detect...
September 2011
Anti-cyclic citrullinated peptide antibodies in rheumatoid arthritis, brucellar arthritis and Behcet’s disease
In Behcet’s disease (BD) and Brucellosis, joint involvement is very common and it may share similarities with rheumatoid arthritis (RA). Anti-cyclic citrullinated peptides (CCP) are evaluated as more specific autoantibodies than rheumatoid factor (RF) in RA. The aim of this study is to determine whether anti-CCP antibodies are useful in the differential diagnosis of RA from other arthritis or not. 153 serum...
September 2011
Survey beta lactamase production and resistance pattern into beta lactame antibiotics in Bacillus cereus strain isolated from staff hands and hospital environment in Iran
Hospital infection is one of the important agent morbidity and mortality in patient. Bacillus cereus is one of the bacteria that cause nosocomial infection. Hospital surfaces and staff hands have important to creation nosocomial infections. Hospital surfaces have potentional capacity to carry bacteria and staff hands are the most reason for the transmission of bacteria to hospital. The present study was...
September 2011
Enzymatic characterization of Malassezia pachydermatis isolates from dogs
The yeast Malassezia pachydermatis is part of the normal cutaneous microflora of most homoeothermic vertebrates. However, under certain conditions as high humidity, seborrheic skin, corticotherapy and immunodeficiency, it can become pathogenic and cause dermatopathies. The pathogenic role of the genus Malassezia seems to be related to physical, chemical and immunological disturbances and to the...
September 2011
Ethnobotanical survey of medicinal plants used in treating viral infections among Yoruba tribe of South Western Nigeria
Ethnobotanical survey of plants used to treat some common viral diseases, such aschicken pox, poliomyelitis, influenza, hepatitis, measles and jaundice was carried out in Ekiti, Ondo, Osun and Oyo States in South Western Nigeria. Questionnaires were distributed among the participants and oral discussions were employed. Two hundred and eight (208) data were collected and tabulated. The Yoruba...
September 2011
Epidemiology of Pseudomonas aeruginosa in Intensive Care Unit and Otolaryngology Department of a Tunisian hospital
Ninety four clinical isolates of Pseudomonas aeruginosa cultured from patients admitted to the Intensive Care Unit (ICU) (N= 37) and Otolaryngology (ORL) (N= 57) during one year (2001-2002) at the Rabta hospital (Tunisia) have been investigated by using serotyping, pyocintyping, drug susceptibility, M13-PCR and PFGE typing. Result shown that most of the isolates at the ICU belonged to serotype O:12 (11/37)...
September 2011
Research on effect of Penicillium sp. in Luzhou-flavor liquor
Penicillium sp. isolated from Daqu was as starting strain. In order to investigate the role ofPenicillium sp. in Luzhou-flavor liquor, Penicillium sp. was operated to Koji seeds and then simulated solid-state fermentation for liquor testing under different conditions, which included adding different amount of Koji seeds and Daqu. The results showed that, when 20%...
September 2011
Cloning and expression of Corynebacterium diphtheriae toxin gene
Diphtheria is an acute bacterial disease caused by oxygenic strains of Corynebacteria. Fatality rate of this disease, between 5 and 10%. In children under 5 years and adults, the fatality rate may be as much as 20%. Outbreaks, although very rare, still occur worldwide, even in developed nations.Diphtheria toxin (DT) is the major virulence factor for these organisms. The aim of this study was cloning and...
September 2010
Theoretical studies on nanostructure of 2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde
The PCM model has been used to optimize 2,66-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde (safranal) in aqueous phase, methanol and carbon tetra chloride at B3LYP/6-31G** level of theory and the solvent effect has been studied. The principle of maximum hardness has been tested by calculating chemical hardness and chemical potential at B3LYP/6-31G** level of theory to predict the order of stability of the structure in...
September 2011
The domestic wastewater (DWW) influence on secondary metabolite in thyme (Thymus vulgaris L.) under field conditions
In order to study the domestic wastewater influence on secondary metabolite in thyme (Thymus vulgaris L.) under field condition, this experiment was carried out in Iran. In the field, 15 lysimeters having thyme (T. vulgaris L.) were used, where 1 to 5 lysimeters wereirrigated by domestic wastewater and primary drainage water was accumulated. 6 to 9 lysimeters were irrigated by primary...
September 2011
Biodegradation of Panicum repens residues by Pleurotus ostreatus for its use as a non conventional feedstuff in diets of Oreochromis niloticus
Biological degradation of torpedo grass, Panicum repens L. residues by Pleurotus ostreatus and suitability of using the highly digestible, protein-enriched, as a non conventional feedstuff in diets of Nile tilapia, Oreochromis niloticus, fingerlings were investigated. The optimal pH and temperature for growth Pleurotus ostreatus and its cellulase production were 6 and 25°C,...
September 2011
Comparison of in vitro degradation of elephant grass and sugarcane by exogenous fibrolytic enzymes
September 2011
Influence of microbial inoculants on soil response to properties with and without straw under different temperature regimes
September 2011
ISO 15189 accreditation experience at the Microbiology Laboratory of a Turkish University Hospital
We aimed to share the experience of the ISO/IEC 15189:2007 accreditation process in a University Hospital’s Medical Microbiology Laboratory before and after the assessment, and tried to seek answers to some questions in applying the standard. Our ISO 15189 accreditation process began in 2008 after having acquired JCI accreditation in 2007. The aim was to be the first accreditated university hospital laboratory in...
September 2011
Biodegradation of free-gossypol by a new fungus isolated from cotton planted soil
A new fungal strain isolated from a soil microcosm was investigated due to the finding that it could biodegrade free-gossypol on agar plates. Benzyl chloride was used for extracting fungal genomic DNA which was isolated from mycelium grown in liquid culture, then 18S rDNA was specifically polymerase chain reaction (PCR) amplified using a pair of primers EF4/EF3 (1.4 kb). The strain was further identified as a member of...
September 2011
Antibiotic resistance pattern of Klebsiella pneumoniae and Enterobacter sakazakii isolates from powdered infant formula
Morbidity and mortality of infant infections caused by contaminated powdered infant formula (PIF) have been reported worldwide, and pathogens like Enterobacter sakazakii andKlebsiella pneumoniae are important causative agents. To evaluate the prevalence of antibiotic resistance in E. sakazakii and K. pneumoniae that caused PIF contamination in Chinese market, all the isolates from...
September 2011
Quinolone resistance in Escherichia coli and Salmonella spp. isolates from diseased chickens during 1993-2008 in China
A total of 363 Escherichia coli and 224 Salmonella spp. were isolated from diseased chickens during 1993-2008 in China. The susceptibility to eight quinolones and prevalence of plasmid-mediated quinolone resistance (PMQR) determinants was investigated in these isolates. Among the E. coli isolates obtained during 1993-1999, 65.2% were resistant to nalidixic acid, while more than 50% of...
September 2011
Isolation and identification of hydrocarbons degrading bacteria in soil around Shiraz Refinery
Petroleum hydrocarbons are species of dangerous contaminants in nature. Scientists are looking for many years to find solution for removing contaminants from the soil and water environments. Today, the use of microorganism, for removing crude oil pollution from contaminated sites (bioremediation) is considered by scientists. The purpose of this research is to isolate and identify hydrocarbons degrading bacteria from...
September 2011
Outer membrane vesicle of Neisseria meningitidis serogroup B as an adjuvant in immunization of rabbit against Neisseria meningitidis serogroup A
Neisseria meningitidis serogroup B outer membrane vesicle (OMV) was revealed to be an efficient carrier for polysaccharide antigens such as capsular antigens. Also, OMV of N. meningitidis was shown to be able to induce high levels of antibodies when applied as an adjuvant for T-independent antigens. In this study OMV was used as an adjuvant with group A meningococcal capsular polysaccharide (GAMP) and...
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