September 2015

Investigation of specific microorganisms in the salt lakes of Southern Russia

Specific microorganisms in extreme reservoirs with total dissolved solids of 195.7 to 585.8 g/l in Southern Russia were studied. Halophilic community of phototrophic and heterotrophic microorganisms was found. In the laboratory, an experiment was carried out to study the degradation ability of microorganisms in relation to organic content of waste water fishing enterprises - brines and bitterns (salt solutions). After...

Author(s): Bataeva, Y. V., Dzerzhinskaya, I. S., Astafyeva, O. V., Satkalieva, M. С., Yakovleva L. V., Kondratenko E. I., Magzanova D. K. and Baimuhambetova A. S.

September 2015

Expression and characterization of recombinant cellulases from an isolated Klebsiella

Seven bacteria strains that have a comparable or higher cellulose degrading capability than Cellulomonas fimi were screened out and phylogenetically analyzed. Genes encoding 1,4-endoglucosidase (celJV) and glucoside hydrolase family 8 (celG) were cloned from Klebsiella sp. strain IV-3, and then inserted separately or simultaneously into pET-22b(+), pETDuet-1 (pDin) or pDout that combined pETDuet-1 with the pelB leader....

Author(s): Qian Li, Xiao Hong, Xiwen Chen and Defu Chen,

September 2015

Phytochemical screening and antibacterial properties of selected Nigerian long pepper (Capsicum frutescens) fruits

Five varieties of Nigerian long pepper (Capsicum frutescens) fruits (var. baccatum, chacoense, fingerh, maxima and minima) were studied for their antibacterial activities.  Methods described by Association of Official Analytical Chemists (AOAC) were employed to determine the phytochemicals in the pepper fruits. The Capsicum frutescens fruits showed antibacterial activity (10.33 - 32.66 mm zones of inhibition)...

Author(s): Bello, I., Boboye, B. E. and Akinyosoye, F. A.

September 2015

A novel method to optimize culture conditions for biomass and sporulation of the nematophagous fungus Metarhizium anisopliae SQZ-1-21 and the entomopathogenic fungus Metarhizium anisopliae RS-4-1

Metarhizium anisopliae is a fungus that offers great potential for the biocontrol of a wide range of host species. In this study, we developed a novel two-stage cultivation method to optimize nutrition and environmental conditions for M. anisopliae SQZ-1-21 and M. anisopliae RS-4-1. Both species were cultured on basal medium (19.00 g sucrose, 4.06 g soy peptone, 1.00 g K2HPO4, 0.50 g KCl, 0.50 g MgSO4, 0.01 g FeSO4 and...

Author(s): Li Gao