June 2014
Urinogenital trichomoniasis in women in relation to candidiasis and gonorrhoea in University of Port-Harcourt Teaching Hospital
This study was conducted to identify and estimate the proportion of patients who had trichomoniasis in relation to gonorrhea and candidiasis in two hundred and twenty (220) women aged between 18 and 55 years from the month of February to June 2005. High vaginal swab (HVS) samples from symptomatic and asymptomatic women in University of Port-Harcourt Teaching Hospital were examined in antenatal, gynecology, family...
June 2014
Influence of acetosyringone concentration on induction of carrot hairy root by Agrobacterium rhizogenes
Carrot hairy root induction by two different strains of Agrobacterium rhizogenes- MTCC- 532 and MTCC- 2364 grown in yeast extract mannitol agar medium (YEMA) was studied in carrot (Daucus carota). The maximum hairy root induction was observed when carrot was simmered with 48 h old culture of A. rhizogenes MTCC-532. With different concentrations of acetosyringone (AS) tested, addition of 150 µM acetosyringone was...
June 2014
Production, purification and characterization of polysaccharide lytic enzymes of a marine isolate, Bacillus cereus NRC-20 and their application in biofilm removal
In screening for marine bacterial polysaccharide lytic enzymes, the most potent isolate was identified as Bacillus cereus NRC-20. This strain showed high alginase, dextrinase, pectinase and carboxymethyle-cellulase productivity when production medium was adjusted at pH 6.0 and contains (g/l): 3.0 sodium alginate, 3.0 malt extract, 30.0 jatropha cake and inoculated with 8% (v/v) of a 24 h old cell suspension and...
June 2014
Diversity and association of filamentous fungi in coffee beans under organic and conventional cultivation
Brazil is a country with great biodiversity; however, knowledge of this microbiological wealth is currently insufficient for its utilization in a sustainable manner. Agricultural expansion represents one of the largest current dangers to biodiversity and threatens to cause the extinction of a variety of species. This study therefore aimed to isolate and identify the species of fungi present in coffee beans cultivated in...
June 2014
Bioaccumulation of Fe3+ by bacteria isolated from soil and fermented foods for use in bioremediation processes
It is known that in areas where mining activity exist, a great ecological imbalance with accumulation of iron particles in the soil can occur, which in high concentrations damages cellular structures of plants and microorganisms. Bacteria resistant to high concentrations of iron with the ability to reduce Fe3+ can make it bioavailable as an electron acceptor. The aim of this work was to select bacteria resistant to Fe3+...
June 2014
Bioremediation of Al-Sayyadin Lagoon polluted water using wild and mutant strains of microalgae
In this study, an attempt was made to reduce the pollutants of Al-Sayyadin Lagoon water, which is still the main site of open fishing in Ismailia, Egypt, using the wild and mutant strains of two green microalgae called Chlamydomonas reinhardtii CC1021 and Parachlorella kessleri PC. Four mutant strains were obtained from UV mutagenesis of the two wild types. One mutant strain was from C. reinhardtii CC1021 (CC1021Mut1)...
June 2014
Antimicrobial assay of methanolic crude of Lonicera lanceolata
The objective of this study was to collect information about medicinal plant Lonicera lanceolata. The antimicrobial assay of four fractions (n-hexane, chloroform, ethyl acetate and ethanol) obtained from Lonicera lanceolata were screened against two human Gram-positive (Staphylococcus aureus, Micrococcus luteus) and four Gram-negative pathogen (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella...
June 2014
Evaluation of Bacillus amyloliquefaciens as manure additive for control of odorous gas emissions from pig slurry
Two in-vitro experiments were conducted to evaluate the efficacy of Bacillus amyloliquefaciens culture broth (BA) on reduction of odorous gas emission from pig slurry. In experiment 1, the treatments included control (no spray), water spray, and spray with 1% (BA1), 5% (BA2), 10% (BA3) and 100% (BA4) BA. Each treatment was replicated three times. The only significant difference in NH3 emission was observed at 48 h, when...
June 2014
Optimization of solid-state fermentation conditions for the production of cellulase and its hydrolytic potentials by Trichoderma virride Sn-9106
Trichoderma viride Sn-9106 with high cellulase activity was used to produce enzyme on residues of Chinese herbs as substrate in solid state fermentation. Residues of Chinese herbs and peptone were found to be the best combination of carbon and nitrogen source for the production of cellulase. The nutrient composition of medium was optimized using response surface methodology. A fractional factorial design (33) was...
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