November 2014

Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Candida parapsilosis

The Candida parapsilosis family has emerged as a major opportunistic and nosocomial pathogen. It causes multifaceted pathology in immuno-compromised and normal hosts, notably low birth weight neonates. In the present study, a novel method, known as loop-mediated isothermal amplification (LAMP), was described for the rapid and specific detection of the species, using primer sets derived from the 5.8 S ribosomal RNA gene...

Author(s): Yili Chen and Hongxu Xu

November 2014

The prevalence of Brucella abortus DNA in seropositive bovine sera in Bangladesh

Prevalence of brucellosis has been widely investigated on the basis of serological test in livestock but the information on the prevalence of Brucella species is scarce in Bangladesh. The objective of this work was to determine the prevalence of Brucella species in cattle and buffaloes in Bangladesh. For these purpose, a total of 799 serum samples of cattle and buffaloes were collected from different districts of...

Author(s): Md. Siddiqur Rahman, Md Abu Sayed Sarker, A. K. M. Anisur Rahman, Roma Rani Sarker, Falk Melzer, Lisa D. Sprague and Heinrich Neubauer,

November 2014

Verification of molecular characterization of coagulase positive Staphylococcus from bovine mastitis with matrix-assisted laser desorption ionization, time-offlight mass spectrometry (MALDI-TOF MS) mass spectrometry

Besides Staphylococcus aureus, other coagulase-positive Staphylococcus (CPS) species such as Staphylococcus hyicus and Staphylococcus intermedius are implicated in bovine mastitis etiology. These species are often misdiagnosed as S. aureus. Also, some atypical S. aureus isolates can test negative for coagulase production and consequently be misdiagnosed as coagulase-negative Staphylococcus (CNS). Several currently...

Author(s): Cássia Couto da Motta, Anna Carolina Coelho Marín Rojas, Felipe Carlos Dubenczuk, Larissa Alvarenga Batista Botelho, Beatriz Meurer Moreira, Shana Mattos de Oliveira Coelho, Irene da Silva Coelho and Miliane Moreira Soares de Souza

November 2014

Microbiological quality and safety of street vended raw meat in Jijiga town of Somali Regional State, southeast Ethiopia

A cross sectional study was conducted to determine the microbial quality and safety of street vended raw meats in Jijiga town, Ethiopia. Questionnaire was used to assess the profile of 33 street vendors. A total of 60 meat samples (30 beef and 30 goats) were collected. The pH and holding temperature were measured. Six microbial groups were counted using standard methods. The aerobic mesophilic flora was characterized....

Author(s): Firew Tafesse, Gulelat Desse, Ketema Bacha and Haile Alemayehu