January 2011
Pathogens change in spontaneous bacterial peritonitis patients with cirrhosis
The aim of this study was to determine the distribution of clinical microorganisms isolated from spontaneous bacterial peritonitis patients. Ascitic fluid was collected from these patients and cultured for pathogens. The patients were placed into three groups, Group A (01/1996 to 12/2000), B (01/2001 to 12/2005) and C (01/2006 to 06/2010) and the clinical data were compared among these groups. There was a...
January 2011
Immunomodulatory effects of novel bifidobacterium and lactobacillus strains on murine macrophage cells
The principle purpose of this study was to investigate the immunomodulatory ability of three microbial strains isolated from Bama County centenarians in activating macrophages, and to characterize the effective component of these strains. A murine macrophage cell line, RAW264.7, was cultured in presence of intact bacterial cells, bacterial cell wall (CW) or cell free extract (CFE). NO, IL-6, TNF-α...
January 2011
Effect of roasting, boiling and microwaving cooking method on sulfadiazine + trimethoprim residues in edible tissues of broiler by microbial inhibition method
The purpose of this study was to determine the effects of different cooking processes like boiling, roasting and microwaving on sulfadiazine + trimethoprim residues in edible tissues of broiler chickens. Each of chicks was fed by water and food with 0.05% of sulfadiazine + trimethoprim in their drinking water for consecutive 5 days. After the 5th day of the drug administration, they were killed and breast...
January 2011
Traditional Iranian dairy products: A source of potential probiotic lactobacilli
A varied climate in Iran makes the production of a wide range of dairy products possible. The aim of this study was to isolate and identify new potential probiotic lactobacilli from traditional Iran dairy products. The isolates were screened for their probiotic potential activities, including acid and bile resistance, antagonistic activity and cholesterol removal. Screening of acid and bile tolerant strains...
January 2011
A magnetic microparticle-based immunoassay for hepatitis C virus NS3 antigen
In order to detect Hepatitis C virus NS3 antigen (HCAg-NS3) in human serum, we developed a magnetic microparticle (MMP) based immunoassay by using biotin-streptavidin amplification, MMP in combination with immunoassay. Results showed that the HCV NS3 monoclonal antibody had a high immobility rate (87.1%) on the surface of MMPs; the assay procedure required smaller volumes of reagents (only 20 μl of immune...
January 2011
Identification of clusters in tissue samples in gene expression data with Principal Component Analysis based on relative variance matrix
Principal Component Analysis (PCA) has been in use as a preprocessing step to clustering for long. We have focused on the clustering of tissue samples in gene expression data. Different clustering techniques and algorithm are available in literature on gene expression data but with the existing ambiguity on the number of clusters, apart from relying on biologically known groups. A consensus is needed to reach...
January 2011
High prevalence of CTX-M-type beta-lactamase in Escherichia coli isolates producing extended-spectrum beta-lactamase (ESBL) and displaying antibiotic co-resistance
In the present study, we investigated the prevalence of CTX-M-type beta-lactamase in extended spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated in our hospital as well as their antibiotic resistance and co-resistance rates. Two hundred nineteen E. coli isolated from clinical specimens between 2006 and 2007 were included. Antibiotic susceptibility test was performed using...
January 2011
Protective effect of flavonoids from pericarpium citri reticulatae (chenpi) against oxidative stress induced by exhaustive exercise
The present study aims at exploring the effects of flavonoids from pericarpium citri reticulatae (FPCR) against oxidative stress induced by exhaustive exercise in rats. Male Sprague-Dawley rats were used in experimental research. Thirty-two rats were randomly divided into four groups that is control (C) group, low-dose FPCR treated (LFT) group, middle-dose FPCR treated (MFT) group and high-dose FPCR...
January 2011
Effect of prebiotic oligosaccharides on growth of Lactobacillus strains used as a probiotic for chickens
In the present study, the effect of 10 commercially available oligosaccharides on the growth of 11 Lactobacillus strains, which were isolated from the gastrointestinal tract of chickens and have been used as a multistrain probiotic for chickens, was evaluated in vitro. The utilization of oligosaccharides was highly variable among the 11 Lactobacillusstrains and considerable strains...
January 2011
Immunogenicity of different cellular fractions of Vibrio parahaemolyticus strains grown under sub-lethal heat and osmotic stress
Vibrio parahaemolyticus is an estuarine bacterium widely distributed in natural aquatic environments and also a well-known food-borne pathogen, and like other enteropathogenic bacteria, it survives both in its natural environment and in its host. The transition from one state to another requires a high capacity to adapt to different physico-chemical parameters, process which increases the synthesis of...
January 2011
β-lactam antibiotic resistance in Escherichia coli commensal faecal flora of healthy population in Taif, Saudi Arabia
One hundred and twenty faecal sample of commensal Escherichia coli strains were collected from different healthy persons and tested for their susceptibility to 12 β-lactam antibiotics by disc diffusion and minimum inhibitory concentration methods. Colonization with strains resistant to ampicillin (Amp), amoxicillin, carbenicillin and peperacillin was detected in 36.7% of the tested isolates....
January 2010
The use of extracts from four local Nigerian plants for the staining of selected bacteria and moulds
Dyestuffs from four Nigerian plants were extracted and their staining ability on some species of bacteria and moulds were determined. The dyestuffs were extracted using water and organic solvents. Acetic acid treatment of the extracts increased the ability of the extract to stain both bacteria and moulds. Treatment with ammonium hydroxide reduced the staining of the plant extracts except the zobo (Hibuscus...
January 2011
Isolation and identification of aerobic, septicemic bacteria from cattle in and around Sebeta town and antimicrobial susceptibility testing
Septicemia is one of the infectious diseases which have zoonotic and economic importance all over the world. Therefore, this study isolated and identified some of the aerobic, septicemic bacteria and then antimicrobial susceptibility test was done in the period November 2006 to May 2007 on 830 sick cattle of different age groups, which were brought to Sebeta veterinary clinic from Sebeta and its surrounding...
January 2011
Influence of five industrial pollutants on mycorrhizal colonization, nodulation and growth of Acacia nilotica and Leucaena leucocephala
An attempt was made to investigate the effect of different polluted soils on the mycorrhizal association, nodulation and subsequent growth of Acacia melanoxylon and Leucaena leucocephala tree species. Five industrial pollutant affected soils viz., Organic chemicals, pesticide, detergent, paper and neem seed oil cake were employed in this study and Palamuru University Campus soil used as a...
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