September 2012
Evaluation of the anti-bacterial and anti-tumour activity of two Chroman -4- one derivatives
In this study, the antibacterial activities of two synthetic chalcones were investigated against Ecsherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeroginosa, Klebsiella pneumonia and Micrococus luteus by agar dilution method. The potato disc method was used to investigate the antitumor activity of the compounds. This assay is accepted as a primary general screening...
September 2012
Cloning, characterization and expression analysis of HSP70 gene from sweet potato
The 70-kDa heat shock protein is a key factor for stress resistance and cellular network of plant. It plays an important role in protein assembly, folding, localization, degradation, tumor immunity etc. In this study, the complete HSP70-coding sequences were cloned from sweet potato by RT-PCR. They contain an open reading frame (ORF) of 1959 bp coding for a peptide of 652 amino acids with a molecular mass of 71.39...
September 2012
Cloning and preliminary identification of SptR, a LuxR-like regulator from Serratia plymuthica
N-acylhomoserine lactone (AHL)-mediated Quorum sensing (QS) allows bacterial populations to communicate via the exchange of diffusible signals to coordinate gene expression and control adaptive behaviors in response to cell density in Gram-negative bacteria. Two luxIR-like QS systems splIR and spsIR have been characterized in an endophytic strain G3 of Serratia plymuthica....
September 2012
Identifying yeasts belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media
The purpose of this work was to compare and optimise different selective and differential media to aid in isolating spoilage yeasts belonging to the Brettanomyces/Dekkera genera. Growth media containing selective and differential factors were employed. These were inoculated with strains of yeast representing Spanish oenological microbiota. Lastly, some of these isolation media were successfully applied in 24 types of...
September 2012
Antioxidant activity of lactic acid bacteria (LAB) fermented skim milk as determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferrous chelating activity (FCA)
Bioactive peptides can be generated from milk protein by fermentation with lactic acid bacteria. In this study, lactic acid bacteria (LAB) were isolated from different food samples. Isolates that showed clear zone on modified de Man, Rogosa and Sharpe (MRS) - CaCO3 agar, catalase negative and Gram positive were considered as LAB and used for this study. Seven isolates that showed proteolytic activity on skim milk...
September 2012
Anti-bacterial and anti-fungal investigation of Astragalus atropilosulus subsp. abyssinicus
Antimicrobial properties of Astragalus atropilosulus subsp. abyssinicus leaf extract were carried out against some pathogenic bacteria and fungi by detecting the zone of inhibition using agar well diffusion method. Fresh A. atropilosulus subsp. abyssinicus leaf extracts were prepared using various solvents (hot and cold water, acetone, ethanol, methanol, 1/1 ethanol/methanol, 1/1...
September 2012
Investigation on the effects of different levels of Citrus sinensis peel extract on gastrointestinal microbial population in commercial broilers
The experiment was conducted to evaluate the effects of different levels of Citrus sinensispeel extract (CSPE) on gastrointestinal microbial population of broilers. Four hundred Ross 308 one-day broilers in a completely randomized design with five treatments (fourreplicates per treatment and each...
September 2012
Identification of pyruvyl groups in a novel microbial polysaccharide
A novel microbial exopolysaccharide (EPS) was isolated from Enterobacter cloacaeZ0206. The pyruvyl groups of the polysaccharide were identified by ultraviolet (UV) spectra, nuclear magnetic resonance (1H NMR, 13C NMR) spectra, quadruple time-of-flight electrospray ionization mass spectrometry (Q-TOF-ESI-MS) and reversed phase-high performance liquid chromatography (RP-HPLC). To measure the pyruvyl groups, a...
September 2012
Differential cultivation and enumeration of Lactobacillus plantarum (PTCC 1058) in the presence of yogurt starter bacteria
This study was aimed at assessing the different selective media and incubation conditions for achieving an appropriate method in the isolation and enumeration of Lactobacillusplantarum PTCC 1058, Lactobacillus delbrueckii ssp. bulgaricus and Streptococcusthermophilus in probiotic yogurt. The yogurt samples containing 1% of L. plantarum PTCC 1058 were prepared...
September 2012
Diversity and phylogenetic analyses of nicotine-degrading bacteria isolated from tobacco plantation soils
Eighteen nicotine-degrading bacteria, which could utilize nicotine as the sole carbon and nitrogen sources, were isolated from tobacco plantation soils in Mile, Yunnan Province, China. These bacteria were identified as Arthrobacter sp. (six strains), Ochrobactrum sp. (five strains), Sphingobacterium sp. (three strains), Sinorhizobium sp. (one strain),Alcaligenes sp. (one...
September 2012
Effects of asphalt on the enzymatic activity and bacterial community in soil
Asphalt is an important material widely used in the coating of surfaces such as roads, roofs, linings of water basins and pipes, but it can be potentially hazardous to human and environment because certain substances exist in asphalt, including polycyclic aromatic hydrocarbons. However, it is unknown about the effect of asphalt on soil. The aim of this study is to examine the effects of asphalt on the physiochemical...
September 2012
Pathogenicity of some isolates of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorokin on 2nd and 4th larval instars of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), under laboratory conditions
The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is one of the major economically pests of potato throughout the world. In this study, the pathogenicity of six isolates of Beauveria bassiana (Bals.) Vuill. (four indigenous and two non indigenous isolates) and two indigenous isolates of Metarhizium anisopliae (Metsch.) were investigated. Five different concentrations from...
September 2012
Isolation and identification of endophytic bacteria of bananas (Musa spp.) in Kenya and their potential as biofertilizers for sustainable banana production
This study was conducted with the aim of isolating and identifying endophytic bacteria associated with bananas in Kenya and assessing their functional potentiality as biological fertilizers. Banana material was collected from two different banana cultivars in five different geographical regions and bacteria were isolated using five different isolation media. Whole-cell matrix-assisted laser desorption/ionization time of...
September 2012
A simple and rapid method for analysis of the immunological effects of beta-glycans in serum and intra-haemocytic components of mangrove crab (Scylla serrata)
A simple and rapid method for analysis of the immunological effects of beta-glycans in mangrove crab (Scylla serrata) was established. Haemocytes were isolated from haemolymph and stained with 20 mg/mL 3, 3-dihexyloxacarbocyanine (DiOC6) a fluorescent and lipophilic dye dissolved in Hanks’ balance salt solution at 4°C for 10 min. After staining, flow cytometry was used to detect the changes in percentages of...
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