May 2014
Genetic diversity and phosphate solubilizing ability of Triticum aestivum rhizobacteria isolated from Meknes region, Morocco
The objective of this work was to isolate, screen and evaluate in vitro, the phosphate solubilization activity of rhizobacteria isolated from Triticum aestivum rhizosphere soil in the Meknes region of Morocco. Five best isolates from 240 ones were selected based on the solubilization of insoluble phosphates (Ca3 (PO4)2) in both agar plate and broth assays using National Botanical Research Institute's phosphate...
May 2014
Comparative evaluation of cellulase activity in Trichoderma harzianum and Trichoderma reesei
Cellulase activity of two promising species of Trichoderma harzianum and Trichoderma reesei were assessed for agro-industrially important cellulase (E.C.3.2.1.4) production. Both species were used for media optimization studies and effect of pH, temperatures and incubation periods on cellulase activity. The maximum cellulase activity was found to be 1.76 U/ml (EXG) in T. reesei at pH 4 in comparison with T. harzianum...
May 2014
Pectate lyase production at high and low pH by Colletotrichum gloeosporioides and Colletotrichum acutatum
Unripe avocado fruit in Mexico are affected by Colletotrichum spp. Although the literature indicates that unripe fruit are resistant to the fungus because of presence of compounds in the fruit, there is evidence on the intervention of pectate lyase (PL) as a virulent factor in Colletotrichum gloeosporioides. The objective of this work was to detect PL from the fungus in response to avocado cell wall under two pH...
May 2014
Development of a loop-mediated isothermal amplification assay for rapid detection of Streptococcus suis serotype 2
The development of a loop mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Streptococcus suis, an economically important swine pathogen is reported. Primers were designed targeting conserved region of the capsular polysaccharide (cps2J) gene of S. suis serotype 2 and 1/2. The LAMP assay produced reliable amplification in 60 min at isothermal conditions of 60°C. Genomic DNA...
May 2014
High viral load is necessary to detect human immunodeficiency virus by polymerase chain reaction in blood-soiled needles
The estimated risk of contamination through percutaneous injuries involving HIV-contaminated sharp objects is 0.32%. It is well known that a high viral load is one of the conditions that increase the risk of HIV-1 transmission in occupational accidents. In order to estimate the level of viral load that could be necessary for HIV-1 transmission to health care workers undergoing occupational accidents with contaminated...
May 2014
The identification of Bordetella bronchiseptica from canine isolates of sequencing 16S ribosomal DNA
The amplification and sequencing of 16S rDNA are useful tools to identify important bacteria that cause diseases in the respiratory tracks of a variety of domestic animals. Bordetella bronchiseptica, one of such bacterium, has demonstrated some changes in its metabolic core, generating a greater need for identification through amplification and sequencing of 16S rDNA. We applied this methodology to eleven nasal and...
May 2014
The immunomodulatory effect of Lactobacillus plantarum strains on mononuclear cells isolated from human peripheral blood
The immunomodulatory effect of nine strains of Lactobacillus plantarum by stimulation of human peripheral blood mononuclear cells (hPBMCs) from healthy donors was investigated. The production of pro-inflammatory, anti-inflammatory and regulatory cytokines by mononuclear cells following exposure of live bacterial cells was measured using multiplex analysis Luminex. The stimulation and production of cytokine by hPBMCs was...
May 2014
Phenotypic and genotypic identification of extended spectrum β-lactamases (ESBLs) among clinical isolates of Escherichia coli
Extended spectrum β-lactamases (ESBLs) are emerging, diverse group of plasmid - associated antibiotic resistant enzymes that are presenting a public health concern. ESBLs were detected in Escherichia coli by performing phenotypic tests on 18 out of 35 E. coli isolates recovered from urine samples of patients with urinary tract infections in three Eygpt hospitals and identified as positive ESBLs according to CLSI...
May 2014
Influence of methanol fruit and leaf extracts of Myristica fragrans (Myristicaceae) on the activity of some antibiotics
The emergence of bacterial resistance to antibiotics is a serious draw back in the management of infections. In this study, the antibacterial activity of the methanol fruit and leaf extracts of Myrisctica fragrans Houtt. (Myristicaceae) against typed strains of Staphylococcu saureus, Bacillus suibtilis, Enterococcus faecalis, Proteus vulgaris, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa were determined...
May 2014
Characterization of different isolates of Zucchini yellow mosaic virus from cucurbits in Saudi Arabia
During the 2012 growing season, thirty three samples were collected from squash, pumpkin and muskmelon plants showing virus-like symptoms in Riyadh and Al-Madena regions of Saudi Arabia. Eleven of these samples were found positive for Zucchini yellow mosaic virus (ZYMV) by double antibody sandwich ELISA (DAS-ELISA). In the host range study for the five selected ZYMV isolates, 11 out of the 22 mechanically inoculated...
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