May 2012
Investigation of succession of bacterial flora in the goose gastrointestinal tract using culture-based methods
The objectives of this study were to investigate the succession of eight bacterial species in the different parts of the goose gastrointestinal tract (GIT) with age using culture-based methods. At 0, 1/4, 1/2, 1, 3, 5, 7, 11, 18, 25, 32, 39, 46, 53 and 360 days of age, six geese were randomly selected and killed by cervical dislocation and glandular stomach, duodenum, jejunum, ileum and caecum samples were...
May 2012
Characterization of drug-resistance and molecular epidemiology of Enterobacter cloacae among patients with clinical infections in a teaching hospital, China
A total of 114 non-repetitive Enterobacter cloacae isolates were collected from patients with clinical infections and tested for susceptibility to 15 antimicrobials. Enterobacterrepetitive intergenic consensus (ERIC) analysis was used to determine the genetic relatedness between isolates with enzymes. Touchdown polymerase chain reaction (PCR) and single PCR were used to detecting the genes...
May 2012
Microflora of biofilm on Algerian dairy processing lines: An approach to improve microbial quality of pasteurized milk
Bacterial contamination of pasteurized milk may originate from different sources: raw milk, process equipment surfaces and packaging materials. It is hypothesized that post-pasteurization contamination along the milk processing lines is responsible of reducing shelf life of Algerian pasteurized milk. This assumption was investigated through assessment of both the microflora of biofilms in milk pipeline...
May 2012
Optimization of the alpha amylase production from Bacillus amyloliquefaciens IIB-14 via parameter significance analysis and response surface methodology
In this article, we report the optimization of bacterial extracellular alpha amylase production from Bacillus amyloliquefaciens IIB-14, a locally isolated bacterial culture, using response surface methodology (RSM). Different agricultural by-products were evaluated for enzyme activity under solid state fermentation (SSF). Of those, wheat bran (10 g) was found to be optimal when moistened with 0.02 M...
May 2012
Improved productivity of recombinant human-like collagen ii by supplying amino acids encoded by rare codons
Amino acids, the key growth factors, affect cell growth and metabolism directly or indirectly. In order to enhance the production of human-like collagen (HLC) II, amino acids were added into initial fermentation broth and response surface methodology (RSM) was used to screen the suitable varieties of amino acids and optimize their doses. Single-factor experiment showed that arginine, proline, threonine,...
May 2012
Bone marrow mesenchymal stem cell from chronic hepatitis B patients differentiation into hepatocyte-like cells
The aim of this study was to observe the biological characteristics and hepatic differentiation potential of bone marrow mesenchymal stem cells (BM-MSCs) from chronic hepatitis B patients in vitro. MSCs were isolated from bone marrow of 15 chronic hepatitis B patients and 11 normal donors by density gradient centrifugation. The adipogenic and osteogenic differentiation potential, immunophenotyping and...
May 2012
Carbapenem resistance in Turkey: Repeat report on OXA-48 in Klebsiella pneumoniae and first report on IMP-1 beta-lactamase in Escherichia coli
Meropenem resistant Klebsiella pneumoniae and Escherichia coli isolates were analyzed. A total of 18 non-duplicate meropenem resistant K. pneumoniae and one E. coli isolates from hospitalized patients were analyzed by polymerase chain reaction (PCR) and DNA sequencing to detect the OXA-48, IMP, VIM, and SPM-1 genes. Susceptibilities to antimicrobials were determined...
May 2012
Studies of actinomycetes for biological control of Colletotrichum musae pathogen during post harvest anthracnose of banana
For the studies and preliminary screening, eighty actinomycetes isolates were selected as antagonists against Colletotrichum musae, causal agents of anthracnose of banana. Actinomycetes isolates were cultured with C. musae (dual culture) to determine their in vitro antagonistic ability. Seventeen strains that strongly inhibited hyphal growth of the pathogen were selected as potent...
May 2012
Vertical shifts in the microbial community structure of organic-rich Namibian shelf sediments
This study investigates the diversity and abundance of bacteria in organic-rich Namibian shelf sediments from two sampling stations, using the 16S rRNA library approach and Catalyzed Reporter Deposition Fluorescent in situ Hybridization (CARD-FISH). Six clone libraries were constructed. Clone libraries were dominated by Delta-proteobacteria (up to 48%) and Gamma-proteobacteria (up to 98%)....
May 2012
Effect of land use system on Arbuscular Mycorrhiza fungi in Maasai Mara ecosystem, Kenya
Arbuscular mycorrhiza fungi (AMF) diversity and inoculums potential were assessed in different land use systems (protected and unprotected grassland and woodland, intensified monocropping systems and subsistence farming systems) in dry and wet region of Maasai Mara ecosystems (MME) during dry and wet season (November, 2009 and April, 2010). AMF spore were assessed in field and trap cultures (sorghum)...
May 2012
Tetracycline resistance genes in Pasteurella multocida isolates from bovine, ovine, caprine and swine pneumonic lungs originated from different Greek prefectures
The aim of the present work was to analyze the antibiotic resistance of Pasteurella multocida isolates from sheep, goats, cattle and pigs originated from eight different Greek prefectures, to determine the class(es) of the tet genes, to investigate any possible association with plasmids or/and chromosomes and to study the geographical distribution of the resistant isolates....
May 2012
Potential microbial candidate strains for management of nutrient requirements of crops
Zinc (Zn) and phosphorus (Pi) are essential for optimum plant growth. Upon application, they get fixed in the soil matrix. Application of more than one microbe for each nutrient is often difficult. Azotobacter (31), Azospirillum (38), Bacillus (19) and Pseudomonas (82) strains were isolated from diverse crop production systems and were evaluated for solubilization of...
May 2012
Seroprevalence of Toxoplasma gondii infection in dairy cattle, in Jinzhou, Northeastern China
The seroprevalence of Toxoplasma gondii infection in dairy cattle was surveyed in Jinzhou City, Northwestern China, between September and October 2011. In total, 350 serum samples were collected from 5 Holstein dairy cattle farms located in Jinzhou. Antibodies to T. gondii were found in 24 (6.86%) of 350 dairy cattle using indirect hemagglutination (IHA) test, with titers of 1:64 in 22,...
May 2012
Isolation and identification of biodegradation ability of alkane-degrading bacteria: Molecular detection and analysis of alkane hydroxylase genes
This study was conducted to characterize and to detect the alkane degradation capacity of the bacterial strain (strain Y9) isolated from the sea mud of the crude oil‑polluted Dinghai area in China. A Gram-negative, strictly aerobic, oxidase negative, and catalase positive bacterium, strain Y9 was isolated and identified as Acinetobacter sp. based on its physiological characteristics and its 16S...
May 2012
Prevalence of Entamoeba histolytica in Iran during 1988 to 2009: Systematic review and meta-analyses
Intestinal parasitic infections are very important for health system in many countries. No published meta-analyses have been assessed in Iran about determination of prevalence of Entamoeba histolytica. Using the main keywords of amebiasis including E. histolytica, prevalence, Iran and intestinal parasites, we searched databases such as Iranmedex, Magiran, Irandoc, Pubmed and Google scholar, and...
May 2012
Trimetazidine treatment influence on pressure overload induced ventricular remodeling and heart failure in rats
In this study, we characterized the therapeutical effects of trimetazidine on subjection to coronary artery ligation, and applied proteomic approach to investigate its potential mechanism of action. Measurement for serum levels of blood pressure, organs index, left ventricular (LV) wall thickness, P53 mRNA and Caspase-3 mRNA expression, heart function and histological study for infarct area of heart were...
May 2012
Compatibility of Metarhizium anisopliae with different insecticides and fungicides
A study was designed to assess the toxicity of insecticides and fungicides on mycelial growth and spore production of Metarhizium anisopliae. All insecticides significantly inhibited mycelial growth and spore production of the fungus. Chlorpyrifos, match, profenofos and metalaxyl+mancozeb were the most toxic chemicals to mycelial growth and conidial germination followed by emamectin, cypermethrin,...
May 2012
Salmonella enterica serovar Typhi plasmid pRST98 enhances caspase-3 mediated macrophage apoptosis by suppressing nitric oxide production
Plasmid pRST98 is a hybrid antibiotic resistance-virulence plasmid isolated fromSalmonella enterica serovar Typhi (S. Typhi). Previously, we transferred pRST98 into attenuated Salmonella enterica serovar Typhimurium (S. typhimurium) strain RIA to create a transconjugant pRST98/RIA and indicated that pRST98 could enhance apoptosis of infected murine macrophages J774A.1....
May 2012
Indigenous wild medicinal plants used by local people of Dudial area, District Mirpur, Azad Jammu and Kashmir, Pakistan
An ethnobotanical study of common medicinal plants of Dudial area, district Mirpur AJK was conducted to investigate the traditional medicinal knowledge of plants. A total of 35 plant species belonging to 30 families were identified and recorded with the help of semi-structured and close ended questionnaires. During this study, 11 Hakims and 68 local informers were interviewed and 72 different sites were visited....
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