November 2008
Identification and initial characterization of a copper resistant South African mine isolate
South African mine isolates were screened for resistance to copper, and an isolate showing the highest resistance was identified as Proteus mirabilis, a Gram-negative bacterium, by 16S rDNA gene analysis and Biolog test. A higher minimal inhibitory copper concentration at 150 mg/ml was obtained using Vätäänen-Nine-Salts-Solution as a less complex medium. Studies pertaining to the copper...
November 2008
Incidence of aerobic bacteria and Candida albicans in post-operative wound infections
The aim of the study was to isolate aerobic bacteria and Candida albicans from post-operative wounds, establish the antimicrobial susceptibility pattern of isolated bacterial agents and proffer ways and means for the prevention of post-operative wound infections. A total of 350 swab specimens from post-operative wounds of consenting patients in Central Hospital, Benin City, were screened for the presence...
November 2008
Quantitative effect of ‘abafe’ (Piliostigma thionnigii) and ‘agehu’ (Khaya ivorensis) leaves on the microbial load of dry-yam ‘gbodo’
The quantitative effect of ‘abafe’ (Piliostigma thionnigii) and ‘agehu’ (Khaya ivorensis) leaves (fresh or dried; singly or combined) on the microbial load of dry-yam ‘gbodo’ was studied. The treated samples had lower microbial loads (>10 to 104 cfu/g) (total plate count, fungal count and staphylococcal count) compared to that of an untreated sample...
November 2008
Evaluation of Beauveria bassiana isolates for virulence against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) and their characterization by RAPD-PCR
Beauveria bassiana is a known natural enemy of a number of insect pests of crop plants. Molecular markers provide a means for constructing the molecular phylogeny, diversity and link to virulent phenotypes in order to screen different isolates of any given entomopathogens. Twenty-seven isolates of B. bassianaisolated from different insect hosts and from different geographical origins were characterized...
November 2008
Microbial solubilization of Ogun rock phosphate in the laboratory and in soil
Biological solubilization of rock phosphate is more environmentally friendly than acidulation. There is a need therefore to develop a microbial process that will make phosphorus available for plant use with minimum pollution to the environment. Solubilization of phosphorus (P) from Ogun rock phosphate (ORP) in a defined growth medium and in soil was carried out to evaluate the potential of a mixed culture of soybean and...
November 2008
Antimicrobial susceptibility and genetic relatedness of Salmonella enterica subsp. enterica serovar Mbandaka strains, isolated from a swine finishing farm in Greece
The current study investigated the antimicrobial susceptibility of Salmonella enterica subsp. enterica serovar Mbandaka (Salmonella Mbandaka) isolated from finishing swines in Greece. Pulsed-field gel electrophoresis (PFGE) was used to examine the genetic relatedness of the isolates. The study was carried out for 1 year as part of a project focusing on antimicrobial resistance of salmonellae...
November 2008
Genotyping of bovine viral diarrhea virus using multiplex PCR, with and without RNA extraction
Bovine viral diarrhea virus (BVDV) is a very important viral disease, which constitutes a major problem worldwide due to the carrier state and due to its misdiagnosis with other viruses. The goal of the current study was to detect and serotype BVDV through multiplex PCR with and without RNA extraction, due to the fact that extraction of RNA may be laborious. Blood samples were taken from 100 randomly-selected animals,...
November 2008
In vitro solubilization of insoluble fluorides by selected fungi
The ability of three species of Penicillium, isolated from soil, and Fusarium solanito solubilize insoluble fluorides was studied. All three Penicillium species and F.solani were capable of solubilizing calcium fluoride and fluorite. A single species ofPenicillium and F. solani also solubilized a range of insoluble fluoride compounds. The ability...
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