African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 357

Full Length Research Paper

Development of a sensitive spectrophotometeric method for determination of copper

Homayon Ahmad Panahi1*, Meghdad Karimi1, Elham Moniri2 and Hamidreza Soudi1
1Department of Chemistry, Islamic Azad University, central Tehran campus, Iran. 2Department of Chemistry, Islamic Azad University, Varamin (Pishva) campus, Iran
Email: [email protected]

  •  Accepted: 17 October 2008
  •  Published: 31 October 2008


Simple and rapid spectrophotometeric procedure have been established for quantitation of Cu (II). This method is based on the reaction between  alizarin red S and boric acid in the solution of pH 8.5 to produce a red complex which place between organic and aqueous phases. In presence of Cu (II), the complex of alizarin red S and boric acid is dissociated and new complex between alizarin red S and metal ions formed which can be measured at the optimum wavelength. All variables were studied to optimize the reaction conditions. Regression analysis of beer’s low plot showed good correlation in concentration range 0.6 - 15 µg.mL-1. The molar absorptivity and detection limit are calculated. The interference effects of the foreign cation have been examined.


Key words: Extraction, copper, trace elements, water samples, Alizarin red S.