African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 353

Full Length Research Paper

omputational approach of protein analysis using affinity chromatography: application to proteomics

Allam Appa Rao
International Center for Bioinformatics, Department of Computer Science and Systems Engineering, Andhra University, Visakhapatnam-530003, India
Email: [email protected]

  •  Accepted: 07 September 2007
  •  Published: 30 September 2007


Computerization is necessary to build the database of chromatography coupled mass spectrometric analytical data. On the basis of this proteomic data we can identify the proteins as biomarkers which are expressing dissimilarity between healthy and disease condition. Affinity chromatography is one of the fastest liquid chromato-graphic methods for the separation and purification of biomolecules due to its high molecular specificity. Proteomics and the tools used to identify and understand the biochemistry of proteins and pathways are in a new stage of development and evaluation. This article briefly reviews the computation of chromatography coupled mass spectrometric data and highlighting the role of affinity chromatography.


Key words: Affinity chromatography, proteomics, computational aspects.