African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 368

Full Length Research Paper

Determination of caffeine content of Nensebo coffee beans Southern Ethiopia, using ultra violet-visible (UV/V) is and high performance liquid chromatography (HPLC) methods in Ethiopia

Legesse Adane
  • Legesse Adane
  • Department of Chemistry, College of Natural and Computational Sciences, Hawaassa University, Ethiopia.
  • Google Scholar
Abera Tadesse
  • Abera Tadesse
  • Department of Chemistry, College of Natural and Computational Sciences, Hawaassa University, Ethiopia.
  • Google Scholar
Tegene Tesfaye
  • Tegene Tesfaye
  • Department of Chemistry, College of Natural and Computational Sciences, Hawaassa University, Ethiopia.
  • Google Scholar


  •  Received: 14 April 2021
  •  Accepted: 19 August 2022
  •  Published: 30 June 2023

References

Abdul M, Kazi F, Zainal A, Zakir H (2006). Determination and characterization of caffeine in tea, coffee, and soft drinks by solid

 

phase extraction and high performance liquid chromatography (SPE- HPLC). Malaysian Journal of Chemistry 8(1):045-051.

 

Abebe B, Kassahun T, Mesfin R, Araya A (2008). Measurement of caffeine in coffee beans with UV/vis spectrometer. Food Chemistry 108(!):310-315.
Crossref

 

Abrar S, Nugussie M (2013). Evaluation of coffee quality of Tigray Regional State: Prudence Journal of Agricultural Research 1(2):3-6.

 

Abu T, Teddy T (2014). Ethiopian coffee annual report. Global Agricultural Information Network Gain report (GAIN Report Number: ET 1402).

 

Adepoju AF, Adenuga OO, Mapayi EF (2017). Coffee; botany, distribution, diversity, chemical composition and its management. Journal of Agriculture and Veterinary Science 10(7):57-62.

 

Ahsan F, Bashir S (2019). Coffee Consumption: Health Perspectives and Drawbacks. Journal of Nutrition and Obesity 2(1):1-4.

 

Alemayehu AA (2014). Coffee production and marketing in Ethiopia. European Journal of Business and Management 6:109-121.

 

Ali MM, Eisa M, Taha MI, Ahmed BZ, Ahmed AE (2012). Determination of caffeine in some Sudanese beverages by High Performance Liquid Chromatography. Pakistan Journal of Nutrition 11(4):336-342,
Crossref

 

Andreia C, Bianca I (2014). Determination of caffeine content from different pharmaceutical and natural products. European Chemical Bulletin 5(4):138-141.

 

Antonella S, Bettina R (2019). Consumers' perceptions of coffee health benefits and motives for coffee consumption and purchasing. Nutrients 11(3):653.
Crossref

 

Bae SK (2013). Simultaneous quantification of caffeine and its three primary metabolites in rat plasma by liquid chromatography-tandem mass spectrometry. Food Chemistry 141(3):2735-2742.
Crossref

 

Belay A, Gholap AV (2009). Characterization and determination of chlorogenic acids (CGA) in coffee beans by UV-Vis Spectroscopy. African Journal of Pure and Applied Chemistry 3(11):234-240.

 

Belay A (2010). Measurement of integrated absorption cross-section, oscillator strength and number density of caffeine in coffee beans by integrated absorption coefficient technique. Food Chemistry 121(2):585-590.
Crossref

 

Belete T, Solomon L (2015). Determination of caffeine content and antioxidant activity of coffee. Journal of American Applied Chemistry 3(2):69-76.
Crossref

 

Castro J, Pregibon T, Chumanov K, Marcus RK (2010). Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). Talanta 82(5):1687-1695.
Crossref

 

Choi EJ, Bae SH, Park JB, Kwon MJ, Jang SM, Zheng YF, Lee YS, Lee S, Bae SK (2013). Simultaneous quantification of caffeine and its three primary metabolites in rat plasma by liquid chromatography-tandem mass spectrometry. Food chemistry 141(3):2735-2742
Crossref

 

Clarke RJ, Macarae R (1985). Coffee chemistry: Amsterdam: Elsevier Applied Sciences 1:124-125.

 

Clarke RJ (1980). In Proceedings of the 9th Coll. ASIC, pp. 467-472.

 

Deepak P, Biswasroy P, Kapil KJ, Pradhan R (2017). Qualitative and quantitative analysis of caffeine in some commercial brands of tea consumed in India. Journal of Ayurvedic and Herbal Medicine 3(4):200-204.
Crossref

 

Demissie EG, Woyessa GW, Abebe A (2016). UV-VIS spectrometer determination of caffeine in green coffee beans from Hararghe, Ethiopia, using Beer-Lambert's law and integrated absorption coefficient techniques. Scientific Study and Research: Chemistry and Chemical Engineering, Biotechnology, Food Industry 17(2):109-123.

 

Dobraca A, Jasmina D, Faruk C, Elma O, Aleksandra M, Miroslave S (2016). Significance of determining the caffeine content in energy drinks. Pharmacia 19:22-29.

 

El-Sayed S, Abdel-Hameed MS, Salih AB, Metwally M, Mohammed TA (2013). Determination of caffeine in Arabica coffee by HPLC and AAS for mineral elements. Life Science Journal 10:2847-2856.

 

Ephrem GD, Girma WW, Arayaselassie A (2016). Caffeine in green coffee beans from Hararghe, Ethiopia, using Beer-lambert's law and integrated absorption coefficient techniques. Scientific Study and Research: Chemistry and Chemical Engineering 17:109-123.

 

Ethiopia: Nation Earns Over U.S.$854 Million From Coffee Export.

View

 

Farah A (2012). Coffee constituents. In: Chu YF, editor. Coffee: emerging health effects and disease prevention. Iowa: Wiley-Blackwell/IFT Press, pp. 22-57.
Crossref

 

Farah A (2009). Coffee as a specialty and functional beverage. In: Functional and specialty beverage technology. Paquin P; Woodhead Publishing, CRC Press, England, pp. 370-390.
Crossref

 

Fisseha G, Gosaye G (2018). Chemistry of caffeine in coffee and its determination using UV/Vis spectrophotometer: A review article. Chemistry and Materials Research 10:23-27.

 

Fox GP, Wu A, Yiran L, Force L (2013). Variation in caffeine concentration in single coffee beans. Food Chemistry 61(45):10772-10778.
Crossref

 

Francis FJ, Roberts HR (1999). Willey Encyclopedia of Food Science and Technology, 2nd Edition.

 

Fung YS (1985). Caffeine analysis using different method Luk, S. F. Analyst 110:201-245. 
Crossref

 

Glade MJ (2010). Caffeine - Not just a stimulant. Nutrition 26(10):932-938.
Crossref

 

Gopinandhan NT, Mallikarjun B, Ashwini MS, Basavaraj K (2014). A comparative study on caffeine estimation in coffee samples by different methods. International Journal of Current Research in Chemistry and Pharmaceutical Sciences 1:04-08.

 

Gopinandhan TN, Ashwini MS (2014). A comparative study on caffeine estimation in coffee samples by HPLC and UV/VIS methods. International Journal of Current Research in Chemistry and Pharmaceutical Sciences 1:4-8.

 

Gray J (1998). Caffeine, coffee and health: Nutrition and Food. Sciences 6:314-319.
Crossref

 

Hassen BH (2015). Determinants of coffee export supply in Ethiopia: Error correction modelling approach. Journal of Economics and Sustainable Development 6(5):21-38.

 

Heckman MA, Weil J, De Mejia EG (2010). Caffeine (1, 3, 7-trimethylxanthine) in Foods: A comprehensive review on consumption, functionality, safety, and regulatory matters. Journal of Food Science 75(3):77-87.
Crossref

 

Higdon JV, Frei B (2006). Coffee and health: a review of recent human research. Critical Reviews in Food Science and Nutrition 46(2):101-123.
Crossref

 

Huck CW, Guggenbichler W, Bonn GK (2005). Analysis of caffeine, theobromine and theophylline in coffee by near infrared spectroscopy (NIRS) compared to HPLC coupled to mass spectrometry. Analytica Chemica Acta 538(1-2):195-203.
Crossref

 

Illy E (2002). The complexity of coffee. Scientific American 286(6):86-91. 
Crossref

 

Ivana H, Ana BC, Dunja H, Drazenka K (2011). Comparative study of polyphenols and caffeine in different coffee varieties affected by the degree of roasting. Food Chemistry 129(3):991-1000.
Crossref

 

Jima D (2020). Review on coffee production and marketing in Ethiopia. Journal of Marketing and Consumer Research 67:7-15.

 

Karacan I, Thornby JI, Anch M, Booth GH, Williams RL, Salis PJ (1976). Dose-related sleep disturbances induced by coffee and caffeine. Clinical Pharmacology and Therapeutics 20(6):682-689.
Crossref

 

Kebena G, Tamene T (2017). Determination of caffeine in raw and roasted coffee beans of Ilu Abba Bora zone, South West Ethiopia. Indo American Journal of Pharmaceutical Research 7(9):463-469.

 

Klang M, Wang N, Meoni LA (2002). Coffee intake and risk of hypertension: The John Hopkins Precursors study. Archives of Internal Medicine 162(6):657-662.
Crossref

 

Liew SL, Nik I, Nik D, Osman H (2001). Determination of coffee content in coffee mixtures. Malaysian Journal of Analytical Sciences 7(2):327-332.

 

Maria B, Paulo M, Marine M (2000). Caffeine content determination of Ethiopian coffee Arabica beans. Genetics Molecular Biolology 23:213-215.
Crossref

 

McCusker RR, Fuehrlein B, Goldberger BA, Gold MS, Cone EJ (2006). Caffeine content of decaffeinated coffee. Journal of Analytical Toxicology 30(8):611-613.
Crossref

 

McCusker RR, Goldberger BA, Cone EJ (2003). Caffeine content of specialty coffees. Journal of Analytical Toxicology 27(7):520-522.
Crossref

 

Mekuria T, Neuhoff D, Kopke U (2004). The status of coffee production and the potential for organic conversion in Ethiopia. Conference on International Agricultural Research for Development, University of Bonn. Institute of Organic Agriculture, Katzenburgweg 3, D-53115, Bonn, Germany.

 

Melkamu A. (2015). Ethiopian Highlands: Home for Arabica Coffee (Coffea arabica L.). Tropical lakes in a changing environment: water, land, biology, climate and humans,(eds.) NYSSEN, J, 58-65.

View

 

Mesfine S, Legesse A, Israel A (2018). Determination of caffeine content of Bale coffee using HPLC analysis. Food Sciences Quality Management 23:29-30.

 

Mulu H, Mesfine RA, Bhagwan SC, Estifanos E, Ahmed MM, Hassen M (2018). Correlation between caffeine contents of green coffee beans and altitudes of the coffee plants grown in southwest Ethiopia. Bulletin of Chemical Society of Ethiopia 32:13-25.
Crossref

 

Naegele E (2003). Determination of caffeine in coffee. Food Testing and Agriculture 34:45-51.

 

Nehlig A (1999). Does caffeine lead to psychological dependance? Chemtech 29(7):30-35.

 

Nuhu AA (2014). Bioactive micronutrients in coffee: recent analytical approaches for characterization and quantification. ISRN Nutrition 2014:384230.
Crossref

 

Olbana I, Dunkana N, Chala B (2019). Electrochemical determination of caffeine in laagee coffee (Ethiopia) with electropolymerization of alizarin on glassy carbon electrode. International Journal of Scientific Engineering Research 10: 962 ISSN 2229-5518.

 

Pandurang NP (2012). Caffeine in various samples and their analysis with HPLC- A review. International Journal of Pharmaceutical Science Review and Research 16(2):76-83.
Crossref

 

Pay E (2009). The market for organic and fair trade coffee. Increasing incomes and food security of small farmers in West and Central Africa through exports of organic and fair-trade tropical products. Rome: Food and Agriculture Organization of the United Nations pp. 1-19.

 

Perrone D, Donangelo CM, Farah A (2008). Fast simultaneous analysis of caffeine, trigonelline, nicotinic acid and sucrose in coffee by Liquid Chromatography-Mass Spectrometry. Food Chemistry 110(4):1030-1035.
Crossref

 

Personal communication with Nensebo coffee and Tea Development and Marketing Authority, June 2018.

 

Phan TT, Vlastimil K, Stanislav K (2012). Determination of caffeine contents of coffee brands in the vietnamese market. Journal of Microbiology, Biotechnology and Food Science 1:995-1002.

 

Rofti J (1971). In Proceedings of the 5th Coll. ASIC, pp. 179-200.

 

Sereshti H, Samadi S (2014). A rapid and simple determination of caffeine in teas, coffees and eight beverages. Food Chemistry 158:8-13.
Crossref

 

Showkat AB, Sim SF, Mohamad NM (2015). Spectrophotometric analysis of Caffeine. International Journal of Analytical Chemistry 2015 Article ID 170239 
Crossref

 

Silvarolla B, Mazzafera P, Fazuoli LC (2004): A natural decaffeinated Arabic coffee. Nature 429: 826.
Crossref

 

Singh BR, Wichter MA, Yhal H (1998). Determination of caffeine using Fourier Transform Infra Red. Biochemical Education 26:243-247.
Crossref

 

Smith AP (2005). Caffeine at work. Human Psypharmacology 20:441-500
Crossref

 

Suraj S, Som K, Prashanta P, Krishna P (2016). A Simple HPLC method for determination of caffeine content in tea and coffee. Journal of Food Sciences and Technology of Nepal 9:74-78.
Crossref

 

Svilaas A, Sakhi AK, Andersen LF, Svilaas T, Strom EC, Jacobs DR, Ose L (2004). Intake of antioxidants in coffee; wine and vegetable are correlated with plasma carotenoids in human. Journal of American Nutrition Science 134(3): 562-567.
Crossref

 

Tamiru M, Legesse A, Kibret F (2018). Comparison of caffeine contents of Yirgacheffe and Harar coffee beans using HPLC analysis. Paripex Indian Journal of Research 7:391-395.

 

Tawfik MS, El Bader NA (2005). Chemical characterization of Harar and Berry coffee beans with special reference to roasting effect. Journal of Food Technology 3(4):601-604.

 

Taye K, Ashenafi A, Alemseged Y, Teshome K, Wondiyfraw T (2011). The contribution of coffee research for coffee seed development in Ethiopia. Journal of Agriculture Research and Department 1(1): 9-16.

 

Tsegaye G (2009). Determination of caffeine in coffee using UV/VIS spectrophotometry. A graduate project submitted to the School of graduate studies of Addis Ababa University in partial fulfilment of the

 

requirements for the Degree of Master of Science In Chemistry, Addis Ababa University, Addis Ababa, Ethiopia.

 

Weinberg BA (2001). The World of caffeine. The Scientist and culture of the World's most popular drug, Routledge. New York.

 

Weldegebreal B, Redi-Abshiro M, Chandravanshi BS (2017). Development of new analytical methods for the determination of caffeine content in aqueous solution of green coffee beans. Chemistry Century Journal 11(1):1-9.
Crossref

 

Yigzaw D, Labuschagne MT, Osthoff G, Herselman J (2007). Variation for green bean caffeine, chlorogenic acids, sucrose and trigonelline contents among Ethiopian Arabica coffee accessions. Sinet: Ethiopian Journal of Science 30(1):77-82.
Crossref

 

Zerihun A, Aman D (2019). Quantification of caffeine content in coffee bean, pulp and leaves from Wollega zones of Ethiopia by High Performance Liquid Chromatography. Trends in Phytochemical Research 3:261-274.

 

Zewdu T, Abdisa G, Liboro H (2016). Determination of caffeine level of Wolaita zone by Uv-visible spectrophotometer. Journal of American Applied Chemistry 4(2):59-63.
Crossref

 

Ziyad J, Zerihun G. Girma M (2020). Bird diversity in Nensebo moist Afromontane forest fragment, South Eastern Ethiopia. The Open Ornithology Journal 13(1):1-9.
Crossref

 

Zhang X, Li W, Yin B, Chen W, Kelly DP, Wang X, Zheng K, Du Y (2013). Improvement of near infrared spectroscopic (NIRS) analysis of caffeine in roasted Arabica coffee by variable selection method of stability competitive adaptive reweighted sampling (SCARS). Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 114:350-356.
Crossref