The chromatographic investigation of the chloroform soluble fraction of methanolic root extract of Ochna schweinfurthiana using a combination of silica and sephadex LH-20 led to the isolation of tri-methoxy derivative of lophirone A. The structure of this new compound was determined using both 1 and 2D nuclear magnetic resonance (NMR). The antimicrobial activity of the isolated compound was also investigated using agar diffusion and broth dilution techniques. Clinical isolates obtained from the Department of Medical Microbiology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria were used for the studies. The compound was found to show activity against Staphylococcus aureus, Streptococcus pyrogenes, Pseudomonas aeruginosa, Klebsiella pneumonia and Samonella typhi, but was not active at the tested dose on methicillin resistant S. aureus, Bacillus subtilis, Corynobacterium ulcerans, Escherichia coli and the only fungi tested Candida albicans. The low concentration for minimum inhibitory concentration (MIC, 5 μg/ml) and minimum bactericidal concentration (MBC, 20 μg/ml) suggests the compound has a good antimicrobial activity against the susceptible organisms and validates the ethno medicinal use of the plant in the treatment of various bacterial infections, including infected wounds and typhoid fever.
Key words: Ochna schweinfurthiana extract, antimicrobial, tri-methoxy, lophirone A, nuclear magnetic resonance (NMR).
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