African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Healing effect of the microemulsion enriched with hydroalcoholic extract of Abarema cochliacarpa (Gomes) Barneby & J. W. Grimes (Fabaceae)

Antonio Santos Dias
  • Antonio Santos Dias
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Clívia Rolemberg Andrade
  • Clívia Rolemberg Andrade
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Andre Luiz Lima Meneses Santos
  • Andre Luiz Lima Meneses Santos
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Sabrina Zelice de Moraes
  • Sabrina Zelice de Moraes
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Jymmys Lopes dos Santos
  • Jymmys Lopes dos Santos
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Silvan Silva de Araujo
  • Silvan Silva de Araujo
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Andrea Yu Kwan Villar Shan
  • Andrea Yu Kwan Villar Shan
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Jamylle Nunes de Souza Ferro
  • Jamylle Nunes de Souza Ferro
  • Institute of Biological and Health Sciences, Federal University of Alagoas (UFAL), Maceió, AL, 57072-900, Brazil.
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Marcelo Cavalcante Duarte
  • Marcelo Cavalcante Duarte
  • Department of Pharmacy, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Emiliano de Oliveira Barreto
  • Emiliano de Oliveira Barreto
  • Institute of Biological and Health Sciences, Federal University of Alagoas (UFAL), Maceió, AL, 57072-900, Brazil.
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Anderson Carlos Marcal
  • Anderson Carlos Marcal
  • Department of Morphology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil
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Ricardo Luiz Cavalcante Albuquerque Junior
  • Ricardo Luiz Cavalcante Albuquerque Junior
  • Institute of Chemistry and Biotechnology, Federal University of Alagoas (UFAL), Maceió, AL, 57072-900, Brazil.
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Antonio Euzebio Goulart Santana
  • Antonio Euzebio Goulart Santana
  • Institute of Biological and Health Sciences, Federal University of Alagoas (UFAL), Maceió, AL, 57072-900, Brazil.
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Brancilene Santos de Araujo
  • Brancilene Santos de Araujo
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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Charles dos Santos Estevam
  • Charles dos Santos Estevam
  • Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, 49000-100, Brazil.
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  •  Received: 06 July 2018
  •  Accepted: 23 August 2018
  •  Published: 22 September 2018

Abstract

Abarema cochliocarpa is an endemic plant of Brazil and has been traditionally used as a popular medicine to treat various diseases and mostly as a healing agent. The aim of this study was to evaluate the healing effect of the microemulsion containing hydrometanic fraction of the inner bark (HMF) of the plant, as well as its antioxidant, antitumor and cytotoxic potential. The cytotoxicity of HMF was assessed by the viability of J774 macrophages cells and the antiproliferative activity in lines of human tumor cells. The antioxidant capacity of the formulation was evaluated in vitro by the ability to scavenge the DPPH and the healing effect was evaluated in Rattus novergicus in 7, 14, 21 days. The HMF significantly favored the viability of J774 macrophages in all tested concentrations and it was dose-dependent. The antiproliferative potential of FHM against human tumor cells was not satisfactory. This formulation presented CE50 of 24.87 ± 0.62 µg.mL-1, and healing effect of 55.18 and 100% of wound retraction up to the 7th and 21st days of treatment, respectively. The bioactive compound of HMF isolated by HPLC was identified by 1H and 13C NMR and by literature data as (+)-catechin. The formulation has curative effect. It can be concluded that the phenolic compounds act in the reduction of oxidative stress and contribute to faster healing.

Key words: Antioxidant, phenolic compound, formulation, healing.

 

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