Abstract

Anticholinergics have effects on organs with hemorrhage-reperfusion injury. The present study investigated the benefit effects of penehyclidine hydrochloride (PHC) and anisodamine (ANI) on hemorrhage-reperfusion and lipopolysaccharide (LPS) (two hits) induced acute kidney injury (AKI). Administration of PHC and ANI do not only remarkedly reduced the plasma concentrations of tumor necrosis factor alpha (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6), but also reduced malondialdehyde (MDA) content, myeloperoxidase (MPO) activity, and enhanced superoxide dismutase (SOD) activity. Histopathological observation showed that PHC and ANI treatment markedly relieved renal histopathological damages, and inflammatory cells infiltration. Furthermore, immunohistochemistrical study presented that intercellular adhesion molecule-1 (ICAM-1) was increased after two hits management, which could be attenuated by PHC and ANI treatment. These data indicated that PHC and ANI had the benefit effects on AKI resulting from two-hits in rats. The effects of PHC treatment were better than ANI.

Key words: Anisodamine, penehyclidine hydrochloride, two hits, acute kidney injury.