African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2282

Full Length Research Paper

Effects of Angelica polysaccharide on erythrocyte immunity and marrow hematopoiesis in chickens

Haifeng Hou1,2, Qian Li3, Wanyu Shi1 and Yongzhan Bao1*
1College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, P. R. China. 2Baoding Vocational and Technical College, Baoding 071051, P. R. China. 3Hebei Institute of Animal Science and Veterinary Medicine, Baoding 071000, P. R. China
Email: [email protected]

  •  Accepted: 14 March 2012
  •  Published: 15 April 2012


To investigate the effects of Angelica polysaccharide on erythrocyte immunity and marrow hematopoiesis in chickens, Angelica polysaccharide was used for the study of immunity and hematinic mechanism in order to provide basis for clinical reference. In this experiment, three gradient dosages (50, 100, 150 mg/kg body weight) of Angelica polysaccharide were drenched to the control group and the anemia groups, respectively. The anemia chickling model was made by abdominal injection of cyclophosphamide (CY) for 6 days (80 mg/kg·day).Red blood cell-C3b receptor (RBC-CR1) and red blood cell-immune complex (RBC-IC) rosette rates were measured and analyzed. Ectogenetic semi-solid culture medium of bone marrow hemopoietic progenitor cells was used to observe Angelica polysaccharide and separated serum fromAngelica polysaccharide treatment on the proliferation of colony-forming unit-erythrocyte (CFU-E), burst-forming unit-erythrocyte (BFU-E) and colony-forming unit-granulocyte macrophage (CFU-GM). The results showed that Angelica polysaccharide can significantly increase RBC-CR1 rosette rate in the healthy chicken groups (p<0.01), but had no more effect on RBC-IC rosette rate (p>0.05). At the same time, Angelica polysaccharide can restore the RBC- CR1 rosette rate and the RBC-IC rosette rate caused by cyclophosphamide to the normal level. Serum containing Angelica polysaccharide can significantly facilitate the proliferation on CFU-E (p<0.01), BFU-E (p<0.01) and CFU-GM (p<0.01), but Angelica polysaccharide had no more direct proliferation on CFU-E, BFU-E and CFU-GM. This indicated that Angelica polysaccharide had the proliferation on hemopoietic progenitor cells of marrow by the change of hemopoietic factor.


Key words: Polysaccharide, chicken, erythrocyte, immunity, hematopoiesis.