Abstract

The current study is designed to developed the new polyherbal combinations of emerging medicinal plants Morus alba (Moraceae), Annona squamosa (Annonaceae), Nelumbo nucifera (Nelumbonoceae) and Psidium guajava (Myrtacea) having effective antidiabetic activity with traditionally used medicinal plants. The formulation were also subjected for the determination of quality control parameters like the heavy metal content, thin layered chromatography (TLC) and high performance thin layered chromatography (HPTLC) profile with an aim to establish their protocols. All 3 formulations, (quoted as FA, FB and FC), are prepared from 10 medicinal plants on the basis of reported mode of action of these medicinal plants used in diabetes. Two dose level 200 and 400 mg/kg of formulations were chosen to evaluate the oral glucose tolerance test (OGTT) in normal rats and antidiabetic activity in streptozotocin (STZ) (60 mg/kg) induced diabetic ‘albino wistar’ rats. Glibenclamide (5 mg/kg) was used as standard. Blood glucose level was measured at 0 h, after 3 h on 5, 10 and 15th day. All 3 combinations were compared on the basis of biochemical analysis (total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), urea, creatinine) of blood plasma. Blood glucose level was determined by glucometer (Escencia entrust, Bayer Health Care). Study showed that FB was found extremely significant in reducing the blood glucose level as compared to all others. FB (200 and 400 mg/kg) showed maximum reduction in average blood glucose level (BGL) to 61.2 and 64.02%, with maximum decrease in average blood glucose level to 67 and 61.4 mg/dl near to standard glibenclamide (66.2%). The results obtained herein indicate that all 3 formulations showed effective antidiabetic activity in order FB > FC > FA and FB was proved to be most beneficial combination.

Key words: Polyherbal formulation, antidiabetic therapeutics, medicinal plants, Annona squamosa, Morus alba, Nelumbo nucifera