African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Effect of oral ingestion of an Arctium lappa extract on the biodistribution of the radiopharmaceutical sodium pertechnetate in rats

Rosane de Figueiredo Neves1,2,3,4, Silvana Ramos Farias Moreno1,2,3*, Ana Lúcia Nascimento5, Jorge José de Carvalho5, Gláucio Diré Feliciano1,6, Sebastião David Santos-Filho1, Paulo Roberto do Couto Neves7, Raíssa de Figueiredo Neves8, Aldo da Cunha Medeiros2 and Mario Bernardo-Filho1,9
1Departamento de Biofísica e Biometria, Instituto de Biologia Roberto de Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 20551-130, Rio de Janeiro, RJ, Brasil. 2Programa de Pós-Graduação em Ciências Médicas, Universidade Federal Fluminense, Rua Marquês de Paraná, 303, 24030-210, Niterói, RJ, Brasil. 3Departamento de Patologia, Universidade Federal Fluminense, Rua Marquês de Paraná, 303, 24030-210, Niterói, RJ, Brasil. 4Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Avenida General. Gustavo Cordeiro de Farias, s/n, 59010180, Natal, RN, Brasil. 5Departamento de Histologia e Embriologia, Instituto de Biologia Roberto de Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, 20551-130, Rio de Janeiro, RJ, Brasil. 6Centro Universitário Estadual da Zona Oeste. Laboratório de Análises Químico-Biológicas, Colegiado de Ciências Biológicas e da Saúde, Campo Grande, Rio de Janeiro, RJ, Brasil. 7Secretaria Municipal de Saúde de Silva Jardim, Rua Borges Alfradique, 60, Silva Jardim, RJ, Brasil. 8Universidade Iguaçu (UNIG), Avenida Abílio Augusto Távora, 2134, 26275-580, Nova Iguaçu, RJ, Brasil. 9Instituto Nacional do Câncer, Coordenadoria de Pesquisa, Praça da Cruz Vermelha, 23, 20230-130, Rio de Janeiro, RJ, Brasil.
Email: [email protected]

  •  Accepted: 07 November 2013
  •  Published: 15 November 2013

Abstract

The aim of the present study was to assess the effect of the oral ingestion of an extract of the Arctium lappa (burdock) on the bio-distribution of the radio-pharmaceutical (radio-biocomplex) sodium pertechnetate (Na99mTcO4) in rats. Male Wistar rats (3 to 4 months of age, 329 ± 16 g) were treated with a burdock extract (1 ml, 20 mg/ml, n = 5) or 0.9% NaCl solution (control: n = 5) for 7 days. After this period of time, Na99mTcO4 (3.7 MBq, 0.3 ml) was injected through the ocular plexus. After 10 min, the rats were sacrificed, the organs isolated and counted in an automatic gamma counter. The percentage of radioactivity was calculated per gram of tissue (%ATI/g) or per whole organ (% ATI/organ). Alteration in Na99mTcO4 uptake was observed in liver from 1.72 ± 0.38 to 0.27 ± 0.07 (% ATI/organ, p < 0.05) and % ATI/g in lung (from 0.45 ± 0.40 to 1.02 ± 0.15 % ATI/g), in testis (from 0.12 ± 0.01 to 0.18 ± 0.02 % ATI/g), in tooth (from 0.24 ± 0.08 to 0.06 ± 0.13 % ATI/g), in tongue (from 0.38 ± 0.06 to 0.08 ± 0.16 % ATI/g) and in liver (from 1.07 ± 0.06 to 0.56 ± 0.15) after treatment with burdock. These findings could result from the interaction between components of the A. lappa extract and the radio-biocomplex which may influence the uptake of Na99mTcO4 in some organs of rats. Therefore, precautions are suggested in the interpretation of nuclear medicine results in patients using burdock.

Key words: Arctium lappa (Burdock), biodistribution, sodium pertechnetate, radiobiocomplex.

Abbreviation

AcOE, Ethyl acetate fraction; Na99mTcO4, sodium perthecnetate; MBq, mega becquerel; NaCl, sodium chloride; A. Lappa, Arctium lappa; Tc-99m, technetium-99m