African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2157

Full Length Research Paper

Distinct radical scavenging and antiproliferative properties of Spondias mombin and antioxidant activity-guided isolation of quercetin-3-O-β-D-glucopyranoside and undec-1-ene

Afolabi C. Akinmoladun*
  • Afolabi C. Akinmoladun*
  • Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria.
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Mohammad Faheem Khan
  • Mohammad Faheem Khan
  • Department of Biochemistry, TheFederal University of Technology, Akure, Ibadan, Nigeria.
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Jayanta Sarkar
  • Jayanta Sarkar
  • CSIR-Division of Pharmacology, Central Drug Research Institute, Lucknow 226001, UP, India.
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Ebenezer O. Farombi
  • Ebenezer O. Farombi
  • CSIR-Medicinal and Process Chemistry Division, Central Drug Research Institute, Lucknow226001, UP, India.
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Rakesh Maurya
  • Rakesh Maurya
  • CSIR-Drug Target Discovery and Development Division, Central Drug Research Institute, Lucknow 226001, UP, India.
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  •  Received: 20 January 2015
  •  Accepted: 25 March 2015
  •  Published: 08 May 2015

Abstract

This study evaluated the antioxidative and antiproliferative activities of phytochemical components from the leaves of Spondias mombin. Leaves of S. mombin were macerated in 80% methanol for 72 h and the extract obtained was fractioned sequentially into water, n-butanol, ethyl acetate and dichloromethane. Ability of extract and fractions to scavenge 2,2–diphenyl-1-picryl hydrazyl, nitric oxide and superoxide radicals were evaluated. A colorimetric sulphorhodamine B (SRB) assay was used for measurement of cell proliferation in four cell lines: KB (oral cancer), C – 33A (cervical cancer), MCF – 7 (breast cancer) and A – 549 (lung cancer) with NIH3T3 (mouse fibroblast) as control cell line. The structures of isolated compounds were established by detailed spectroscopic studies. Ethyl acetate and n-butanol fractions showed highest radical scavenging activity while the dichloromethane fraction demonstrated the best anti-proliferative activity. Chromatographic separation of the combined ethyl acetate and n-butanol fractions yielded some compounds out of which quercetin-3-O-β-D-glucopyranoside and undec-1-ene were characterized. The present study demonstrates that the nature of solvent is a crucial variable modulating the availability and activity of phytochemical components and that S. mombin is a potential source of chemically diverse prophylactic and chemotherapeutic agents.

Key words: Antiproliferative activity,bioactive compounds,radical scavenging property,Spondias mombin