African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2296

Full Length Research Paper

EC50 of adrenaline-atenolol: Functional agonist assay using Langendorff isolated rabbit heart tethered to powerLab data acquisition system

Abdullah S. Shatoor1*, Fahaid AL-Hashem2, Abbas Elkarib2, Hussein Sakr2 and Mahmoud Alkhateeb2
1Department of Internal Medicine, Cardiology Section, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia. 2Department of Physiology, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia.
Email: [email protected]

  •  Accepted: 30 March 2012
  •  Published: 22 April 2012

Abstract

The purpose of this study was to determine the mean EC50 of adrenaline on heart rate and force of contraction of isolated New Zealand white rabbit hearts and to determine the concentration of atenolol that completely blocks the effect of adrenaline using one of modern physiological acquisition system, PowerLab, AD instrumnets. Twelve Isolated hearts from New Zealand white rabbits were perfused through aorta in a Langendorff mode. Heart rate and contractility were determined for 5 minutes after bolus injection of 5 different concentrations of adrenaline (1.0, 2.5, 5.0 and 10 µg/mL). The changes in heart rate and contractility after each treatment were compared with their baseline values. These data were used to calculate the mean EC50 of adrenaline on heart rate and force of contraction. This EC 50 was then used after perfusion of different concentrations of atenolol (1.0, 2.5, 5.0 and 10µg/mL). Data were collected with the help of PowerLab data acquisition and analyzed by Labchart pro7 software. Adrenaline resulted in a stimulatory effect on the heart rate and the amplitude of the heart contraction. The maximum increases in both heart rate and force of contraction were seen at adrenaline dose of 7.5 µg/mL and the plateau phase was achieved at a dose of 10 µg/mL.  The average EC50 of adrenaline was 3.5 µg/mL. The positive inotropic effect of adrenaline was antagonized only at atenolol concentrations of 5.0 and 10 µg/mL and complete inhibition of adrenaline effect on heart rate was achieved at atenolol concentrations of 10 µg/mL. These data showed that atenolol must be used at a concentration no less than 7.5 µg/mL to demonstrate if β adrenergic receptors are involved in the mechanism of action of any newly tested positive inotropic or choronotroic drug.

 

Key words: Adrenaline, atenolol, isolated hearts, rabbits.