One hundred and twelve (112) endophytic fungi was isolated from leaf fragments of Annona crassiflora. Extracts were prepared by fermentation in Czapek broth. The extract of endophytic fungus Rhizoctonia sp. was selected as the most promising from an antimicrobial screening and its ability to produce metabolites with antimicrobial and antiproliferative activity was evaluated. Antimicrobial activity was evaluated through agar diffusion and broth microdilution. In the screening, the extract was able to inhibit Staphylococcus aureus, Escherichia coli and Candida albicans with inhibition zone diameters ranging from 27 to 40, but its antimicrobial activity was most prominent against S. aureus, with a minimum inhibitory concentration (MIC) of 500 to 1000 μg/ml. Regarding anti-proliferative activity, the results were promising. The extract displayed potent anti-proliferative activity against all tumor cell lines tested, suggesting the potential capacity to diminish the growth of human tumor cells, while also demonstrating selectivity by displaying reduced activity against non-tumor cell lines.
Key words: Endophytic fungi, Annona crassiflora, fermentation process, bioactive compounds, biological assays.
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