African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2198

Full Length Research Paper

Potential antimicrobial and antiproliferative activity of the crude extract of the endophytic fungus Rhizoctonia sp. from Annona crassiflora

Andrea Natan de Mendonca*
  • Andrea Natan de Mendonca*
  • Faculty of Pharmaceutical Sciences, Federal University of Alfenas (UNIFAL-MG), Alfenas, Minas Gerais, Brazil.
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Barbara Helena Muniz Padro e Feliphe
  • Barbara Helena Muniz Padro e Feliphe
  • Faculty of Pharmaceutical Sciences, Federal University of Alfenas (UNIFAL-MG), Alfenas, Minas Gerais, Brazil.
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Raquel Maria Lima Lemes
  • Raquel Maria Lima Lemes
  • Faculty of Pharmaceutical Sciences, Federal University of Alfenas (UNIFAL-MG), Alfenas, Minas Gerais, Brazil.
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Ana Lucia Tasca Gois Ruiz
  • Ana Lucia Tasca Gois Ruiz
  • CPQBA, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.
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Joao Ernesto de Carvalho
  • Joao Ernesto de Carvalho
  • CPQBA, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.
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Masaharu Ikegaki
  • Masaharu Ikegaki
  • Faculty of Pharmaceutical Sciences, Federal University of Alfenas (UNIFAL-MG), Alfenas, Minas Gerais, Brazil.
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  •  Received: 12 December 2014
  •  Accepted: 02 March 2015
  •  Published: 15 March 2015

Abstract

One hundred and twelve (112) endophytic fungi was isolated from leaf fragments of Annona crassiflora. Extracts were prepared by fermentation in Czapek broth. The extract of endophytic fungus Rhizoctonia sp. was selected as the most promising from an antimicrobial screening and its ability to produce metabolites with antimicrobial and antiproliferative activity was evaluated. Antimicrobial activity was evaluated through agar diffusion and broth microdilution. In the screening, the extract was able to inhibit Staphylococcus aureus, Escherichia coli and Candida albicans with inhibition zone diameters ranging from 27 to 40, but its antimicrobial activity was most prominent against S. aureus, with a minimum inhibitory concentration (MIC) of 500 to 1000 μg/ml. Regarding anti-proliferative activity, the results were promising. The extract displayed potent anti-proliferative activity against all tumor cell lines tested, suggesting the potential capacity to diminish the growth of human tumor cells, while also demonstrating selectivity by displaying reduced activity against non-tumor cell lines.

Key words: Endophytic fungi, Annona crassiflora, fermentation process, bioactive compounds, biological assays.