African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2198

Full Length Research Paper

Two new phenolic constituents from Clematis connata DC.

Lin Yuan1, Wenzhong Huang1, Yinhai Ma* and Zhizhi Du2
1Chemical Science and Technology Department, Kunming University, Kunming 650214, P. R. China. 2State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China.
Email: [email protected]

  •  Accepted: 20 March 2012
  •  Published: 15 April 2012

Abstract

Two new phenolic compounds, 3,4-dihydroxyphenethyl 5-hydroxy-4-oxopentanoate (1) and 3-(4-hydroxy-2,5-dimethoxyphenyl)-3-oxopropyl acetate (2), together with fifteen known compounds, 3 to 17, were isolated from the dried whole plants of Clematis connata DC. Their structures were elucidated by extensive spectral analysis, especially 2D NMR techniques. Compounds 1 to 5 were evaluated for their in vitro cytotoxicities against acute promyelocytic leukemia HL-60, hepatocellular carcinoma Hep-G2, oropharyngeal epidermoid carcinoma KB and breast cancer cells MDA-MB-231. Compounds 1 and 3 exhibited the most potential with IC50 values of 0.63 and 1.27 μM against MDA-MB-231 and HepG2, respectively. The antibacterial activities experiment of all the isolated compounds showed that only Compounds 3 to 5 exhibited weak activities against Staphylococcus aureus, methcillin-resistant S. aureus and b-lactamase positive S. aureus, and Compound 9 and 17 showed weak activities against S. aureus.

 

Key words: Clematis connata DC., phenolic constituents, cytotoxicities, antibacterial activities.