African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2286

Full Length Research Paper

In vitro inhibition of cyclooxygenases, anti-denaturation and antioxidant activities of Malian medicinal plants

Mahamadou BALLO
  • Mahamadou BALLO
  • Laboratoire du Développement du Médicament, Université Joseph Ki-Zerbo, Burkina Faso.
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Karim TRAORE
  • Karim TRAORE
  • Laboratoire d’Immunogénétique du Centre de Recherche et de Formation sur le Paludisme, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Abdoulaye D.A. GUINDO
  • Abdoulaye D.A. GUINDO
  • Faculté de Pharmacie, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Seidina A.S DIAKITE
  • Seidina A.S DIAKITE
  • Faculté de Pharmacie, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Blaise DACKOUO
  • Blaise DACKOUO
  • Laboratoire d’Immunogénétique du Centre de Recherche et de Formation sur le Paludisme, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Raogo OUEDRAOGO
  • Raogo OUEDRAOGO
  • Laboratoire du Développement du Médicament, Université Joseph Ki-Zerbo, Burkina Faso.
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Sékou BAH
  • Sékou BAH
  • Faculté de Pharmacie, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Mahamadou DIAKITE
  • Mahamadou DIAKITE
  • Laboratoire d’Immunogénétique du Centre de Recherche et de Formation sur le Paludisme, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Rokia SANOGO
  • Rokia SANOGO
  • Faculté de Pharmacie, Université des Sciences, des Techniques et des Technologies de Bamako, Mali.
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Estelle N. H. YOUL
  • Estelle N. H. YOUL
  • Laboratoire du Développement du Médicament, Université Joseph Ki-Zerbo, Burkina Faso.
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  •  Received: 15 January 2023
  •  Accepted: 30 January 2023
  •  Published: 28 February 2023

Abstract

Cyclooxygenase 2 (COX-2) is involved in the production of prostaglandins that sustain the inflammatory process. Inflammatory cells release a number of reactive species. Furthermore, reactive oxygen species can initiate intracellular signaling cascades and enhance the expression of pro-inflammatory genes. The objective was to study the cyclooxygenase and protein denaturation inhibition and antioxidant activity of ten extracts from five plants. Cyclooxygenases inhibitory activity of the extracts was measured using the Cayman Kit method and the protein denaturation inhibitory activity using the bovine serum albumin. Ferric reducing antioxidant power and hydrogen peroxide scavenging activity were used to evaluate the antioxidant capacity of aqueous and hydroethanolic extracts. COX-2 inhibition was more important with the hydroethanolic extract of X. americana (IC50 = 11.13 ± 1.24 µg/ml) and T. macroptera (IC50 = 12.79 ± 0.56 µg/ml). COX-1 was strongly inhibited with the hydroethanolic extract of S. senegalensis. The hydroethanolic extracts of C. tinctorium and X. americana showed the strongest inhibitory activities of protein denaturation with 86.61 ± 1.22% and 84.5 ± 0.56% respectively. The greatest effects on iron reduction were observed with the hydroethanolic extracts of X. americana (R2 = 0.996; IC50 = 29 µg/ml) and T. macropteria (R2 = 0.990; IC50 = 35.46 µg/ml). The hydrogen peroxide scavenging activity of the extracts varied from 39.31 ± 3.6 to 77.37 ± 2.16 µg/ml. The present study concluded to an important antioxidant activity, protein denaturation and COX-2 inhibitory activities of the hydroethanolic extracts of X. americana, T. macroptera and C. tinctorium. Further studies are needed to confirm the anti-inflammatory effect of these extracts in vivo.

Key words: X. Americana, T. macroptera, cyclooxygenases, antioxidant, protein denaturation.