African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2281

Full Length Research Paper

Efficacy of different herbal preparations of Azadarachta indica Juss. on a 4-day schizontocidal test on Plasmodium Berghei

J. K. EBIGWAI1*, E. A. EDU1, M. E. ILLONDU2, A. NTA3, U. Udensi4 and E. E. OKU3
1Department of Botany, University of Calabar, Calabar, Nigeria. 2Department of Botany, Delta state University, Abraka, Delta State, Nigeria. 3Department of Zoology, University of Calabar, Calabar, Nigeria. 4Department of Genetics and Biotechnology, University of Calabar, Calabar, Nigeria.
Email: [email protected]

  •  Accepted: 02 December 2011
  •  Published: 22 April 2012

Abstract

A four-day 4 suppressive schizontocidal test for anti malarial activity against Swiss albino mice infected with chloroquine sensitive strain (NK-65) of Plasmodium berghei was used to monitorin vivo response to different modes of preparing herbal remedies using the stem and bark ofAzadarachta indica Juss. Four recognized modes of preparing anti malarial remedies from A. indica were tested against a positive control (chloroquine) and a negative control (phosphate buffer saline). On day zero (0), all the animals received 8 × 10million P. berghei infected red blood cells corresponding to 0.1 ml of the blood sample. The four herbal recipes (infusion, decoction, tincture and juice (macerate)) were prepared in a 1:1 ratio of stem bark and leaves. After 2 h of plasmodium challenge, the experimental animals received oral doses of 125, 250 and 50 mg/kg in triplicates. The recipes had intrinsic anti malarial activities that were dose dependent. The comparison analysis indicated that 250 and 500 mg/kg body weight of the tincture and decoction in addition to the 500 mg/kg body weight yielded 76.34, 85.08, 71.26, 82.43 and 78.94, respectively when compared with the chloroquine with 70.24% suppression. The result were significant (p < 0.001) at p < 0.05 when compared with a placebo and support the use of a dose not less than 125 mg/kg body weight of the tincture and decoction as effective.

 

Key words: Plasmodium berghei, schizontocidal, mode of preparations, chloroquine, phosphate buffer saline.