African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2281

Full Length Research Paper

An in vitro evaluation of the antileishmanial and cytotoxic activity of methyl gallate associated with conventional treatment for cutaneous leishmaniasis

Rebecca Sayuri Barbosa Hanada
  • Rebecca Sayuri Barbosa Hanada
  • University Center of the North, Manaus, AM, Brazil.
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Bruno Bezerra Jensen
  • Bruno Bezerra Jensen
  • Laboratory of Leishmaniasis and Chagas Disease, National Institute for Research in Amazonia, Manaus, AM, Brazil.
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Richaelly Pinheiro dos Santos
  • Richaelly Pinheiro dos Santos
  • University Center of the North, Manaus, AM, Brazil.
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Paula Figliuolo da Cruz Borges
  • Paula Figliuolo da Cruz Borges
  • Laboratory of Leishmaniasis and Chagas Disease, National Institute for Research in Amazonia, Manaus, AM, Brazil.
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Francimeire Gomes Pinheiro
  • Francimeire Gomes Pinheiro
  • Laboratory of Leishmaniasis and Chagas Disease, National Institute for Research in Amazonia, Manaus, AM, Brazil.
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Mayara Arguello da Silva
  • Mayara Arguello da Silva
  • Laboratory of Leishmaniasis and Chagas Disease, National Institute for Research in Amazonia, Manaus, AM, Brazil.
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Ana Caroline dos Santos Castro
  • Ana Caroline dos Santos Castro
  • University Center of the North, Manaus, AM, Brazil.
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Juliana Maria de Morais
  • Juliana Maria de Morais
  • Laboratory of Leishmaniasis and Chagas Disease, National Institute for Research in Amazonia, Manaus, AM, Brazil.
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Antonia Maria Ramos Franco
  • Antonia Maria Ramos Franco
  • Laboratory of Leishmaniasis and Chagas Disease, National Institute for Research in Amazonia, Manaus, AM, Brazil.
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  •  Received: 20 April 2024
  •  Accepted: 21 May 2024
  •  Published: 31 May 2024

Abstract

Cutaneous leishmaniasis (CL) is a non-contagious infectious-parasitic disease caused by protozoa of the genus Leishmania sp. It is considered a neglected tropical disease and involves public health issues because it affects socially vulnerable populations. The therapy recommended by the Brazilian Ministry of Health as the standard treatment for CL presents numerous challenges. Therefore, it is necessary to look for alternative treatments that are more effective and safer without any side effects on the patients. The phenolic compound methyl gallate (MTG) is an alternative candidate for study due to its antileishmanial biological activity. Methyl gallate has shown considerable biological activity against Leishmania amazonensis. Thus, investigating the potential of this compound in combination with Glucantime® and Pentacarinat® against Leishmania species and evaluating its cytotoxic profile is critical in the treatment and management of CL. The experimental design involved in vitro assays with promastigotes of L. amazonensis and L. guyanensis to evaluate the antileishmanial inhibitory activity of methyl gallate in monotherapy and in combination with conventional treatments, as well as to assess its cytotoxic profile using peritoneal macrophages extracted from BALB/c mice. As a result of the antileishmanial activity test, methyl gallate showed parasite inhibitory activity against L. amazonensis and L. guyanensis promastigotes, both in monotherapy and in combination with the conventional CL drugs. Methyl gallate or its combinations did not show any signs of cytotoxicity. These results provide a basis for future studies on this polyphenolic compound as a potential alternative drug for development and adoption in the therapeutic protocol for CL treatment.

Key words: Cutaneous leishmaniasis, neglected tropical diseases, methyl gallate, combination therapy, in vitro assays.