R - (+) - limonene is a monoterpene found in several plant species. Studies have shown that plants that have this compound has anxiolytic activity. This study aimed to investigate the anxiolytic and toxicological activity in rats after inhalation of R - (+) - limonene for 30 min. For the anxiety tests using Elevated Plus Maze (EPM), concentrations of 1.0, 2.5 and 5.0% were determined. From the results, it was observed that the last concentration had achieved anxiolytic effect. Based on these results, it was found by biochemical and hematological tests, toxicity of 5% concentration in the period of 30 days with minor significant changes. In the hematological parameters, MCV, MCH and MCHC values were significant when compared to the control group, but were within the normal range when compared to values found in literature. In the biochemical parameters, changes occurred in AST and LDH levels. Elevated LDH levels can cause destruction of erythrocyte precursor cells in the bone marrow. In turn, AST may be associated to small changes in the liver. Therefore, the results demonstrated an anxiolytic activity with low toxicity at dose of 5% R - (+) - limonene via inhalation for 30 min.
Key words: R-(+)-limonene, inhalation, biochemical, hematological, anxiolytic, toxicity.
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