African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2208

Full Length Research Paper

Preclinical evaluation of repeated dose toxicity and anxiolytic activity of monoterpene R - (+) – limonene via inhalation

Mateus Feitosa Alves
  • Mateus Feitosa Alves
  • Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa – PB, Postal Box 5009, Brazil.
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Vanessa Feitosa Alves
  • Vanessa Feitosa Alves
  • Department of Clinical and Social Dentistry, Federal University of Paraíba, João Pessoa – PB, Brazil.
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Josué do Amaral Ramalho
  • Josué do Amaral Ramalho
  • Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa – PB, Brazil.
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Guilherme Eduardo Nunes Dias
  • Guilherme Eduardo Nunes Dias
  • Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa – PB, Brazil.
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Kardilândia Mendes de Oliveira
  • Kardilândia Mendes de Oliveira
  • Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa – PB, Brazil.
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Andressa Brito Lira
  • Andressa Brito Lira
  • Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa – PB, Postal Box 5009, Brazil.
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Sócrates Golzio dos Santos
  • Sócrates Golzio dos Santos
  • Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa – PB, Brazil.
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Reinaldo Nóbrega de Almeida
  • Reinaldo Nóbrega de Almeida
  • Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa – PB, Postal Box 5009, Brazil.
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Margareth de Fátima Formiga Melo Diniz
  • Margareth de Fátima Formiga Melo Diniz
  • Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa – PB, Postal Box 5009, Brazil.
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  •  Received: 18 June 2016
  •  Accepted: 21 July 2016
  •  Published: 08 August 2016

Abstract

R - (+) - limonene is a monoterpene found in several plant species. Studies have shown that plants that have this compound has anxiolytic activity. This study aimed to investigate the anxiolytic and toxicological activity in rats after inhalation of R - (+) - limonene for 30 min. For the anxiety tests using Elevated Plus Maze (EPM), concentrations of 1.0, 2.5 and 5.0% were determined. From the results, it was observed that the last concentration had achieved anxiolytic effect. Based on these results, it was found by biochemical and hematological tests, toxicity of 5% concentration in the period of 30 days with minor significant changes. In the hematological parameters, MCV, MCH and MCHC values were significant when compared to the control group, but were within the normal range when compared to values found ​​in literature. In the biochemical parameters, changes occurred in AST and LDH levels. Elevated LDH levels can cause destruction of erythrocyte precursor cells in the bone marrow. In turn, AST may be associated to small changes in the liver. Therefore, the results demonstrated an anxiolytic activity with low toxicity at dose of 5% R - (+) - limonene via inhalation for 30 min.

Key words: R-(+)-limonene, inhalation, biochemical, hematological, anxiolytic, toxicity.