African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2281

Full Length Research Paper

Cytotoxic and renoprotective flavonoid glycosides from Horwoodia dicksoniae

Ghada Ahmed Fawzy1,2*, Areej Mohammed Al-Taweel1, Nayira Ahmed Abdel Baky3,4 and Mohamed Sobhy Marzouk5  
1Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 22452, Riyadh 11495, Kingdom of Saudi Arabia. 2Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo, Egypt. 3Department of Pharmacology, College of Pharmacy, King Saud University, P. O. Box 22452, Riyadh 11495, Kingdom of Saudi Arabia. 4Pharmacology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt. 5Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P. O. Box 22452, Riyadh 11495, Kingdom of Saudi Arabia.
Email: [email protected]

  •  Accepted: 29 March 2012
  •  Published: 22 April 2012

Abstract

Three flavonoid glycosides and one aglycone were isolated from the ethanol extract (EE) ofHorwoodia dicksoniae (Brassicaceae), for the first time, and their structures were established from negative ESI-MS, 1H-, 13C-NMR and DEPT as luteolin 7-O-β-D-glucopyranoside (1), luteolin 6-C-β-D-galactopyranoside (2), apigenin 6-C-β-D-galactopyranoside (3) and luteolin (4). The SRB cytotoxicity assay was used to investigate the antitumor activity of the EE, Compounds 1, 2 and 4. Compound 1 showed the highest cytotoxic activity against the three human cell lines, including HEP-G2, HCT-116 and MCF-7 (IC50 = 10.7, 9.3 and 9.9 µg/ml, respectively), compared with doxorubicin as reference drug. Compound 4 showed selective antitumor activity against the colon cell line (IC50 = 9.5 µg/ml). The present investigation also demonstrates the protective effect of Compounds 1, 2 and 4 with strong antioxidant potential, in glycerol-induced myoglobinuric acute renal failure in rats. Moreover, all tested compounds separately attenuated renal dysfunction, and restored the oxidant balance by decreasing renal MDA levels, increasing the activity of the depleted renal antioxidant enzymes, and the non enzymatic antioxidant GSH. They also, decreased the elevated serum inflammatory marker (TNF-α), and ameliorated apoptotic kidney damage by reduction in caspase-3 activity. Taken together, 1 was found to be the most biologically active compound.

 

Key words: Rhabdomyolysis, glycerol, acute renal failure, flavone glycosides, cytotoxicity.