African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2173

Full Length Research Paper

Antiproliferative and cytotoxic activity of Hematite (α-Fe2O3) nanoparticles from Butea monosperma on MCF-7 Cells

B. Behera
  • B. Behera
  • Department of Pharmaceutical Sciences, Utkal University, Vanivihar, Bhubaneswar, Odisha, 751004 India.
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S. Pradhan
  • S. Pradhan
  • Department of Pharmaceutical Sciences, Utkal University, Vanivihar, Bhubaneswar, Odisha, 751004 India.
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A. Samantaray
  • A. Samantaray
  • Department of Pharmaceutical Sciences, Utkal University, Vanivihar, Bhubaneswar, Odisha, 751004 India.
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D. Pradhan
  • D. Pradhan
  • Department of Pharmacy Science, Creighton University, Medical Centre, Omaha, NE, USA.
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  •  Received: 16 December 2019
  •  Accepted: 15 January 2020
  •  Published: 29 February 2020

Abstract

Silver nanoparticles (Hematite (α-Fe2O3) have been used as an antimicrobial and as disinfectant. Nevertheless, there is limited data about its antitumor potential. This study focused on investigating the cytotoxic effects of Hematite (α-Fe2O3) from Butea monosperma flower extract on MCF-7 breast cancer cells and its mechanism of action.  Green method was created for the synthesis of Hematite (α-Fe2O3) using an aqueous extract of B. monosperma flower. Synthesis of hematite (α-Fe2O3) was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-[4, 5-dimethylthiazol-2-yl]-a 2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2',7'-dichlorofluorescein diacetate and intracellular calcium (Cai2+) was evaluated with probe flu3-AM. Cells were treated with different concentrations of hematite (α-Fe2O3) (1, 3, 6, 10, 15, 25, 50 and 100 μg/mL).  The results showed that hematite (α-Fe2O3) hindered cell growth in a dose-dependent manner. Hematite (α-Fe2O3) appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai2+ (IC50 52 ± 3.14).  In conclusion, the results of this preliminary study demonstrated that hematite (α-Fe2O3) from B. monosperma flower extract may be a potential therapeutic potential medicament for human breast cancer treatment.

Key words: Cytotoxicity, MCF-7 cell line, Butea monosperma, Hematite (α-Fe2O3), nanoparticles.